Recombinant Human TGF-beta 1 Monomer Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P01137
蛋白标签: N-His
生物活性: Immobilized Recombinant Human TGF-beta 1 Monomer protein (rp176238) at 1.0 μg/mL can bind Recombinant TGF beta 1 Antibody (Ab130853) with the EC50 of 5 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp176238-10μg	10μg	现货
rp176238-50μg	50μg	现货
rp176238-100μg	100μg	现货
rp176238-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: TGFB1

Accession#:P01137 (10) Gene ID:7040 (10) TGF-beta 1 (3) TGFB1 (12)

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基本描述

产品名称	Recombinant Human TGF-beta 1 Monomer Protein
别名	重组人TGF-beta 1蛋白 \| 重组人转化生长因子β1蛋白 \| 重组人类 TGF-β1 单体蛋白
英文别名	DPD1 \| CEDLAP \| latency-associated peptide \| TGF beta \| TGF beta1 \| TGFB \| TGFB1 \| TGF-beta 1 protein \| TGFbeta 1 \| TGF-beta 1 \| TGFbeta \| TGF-beta-1 \| transforming growth factor beta-1 \| transforming growth factor, beta 1
规格或纯度	ActiBioPure™, 生物活性, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	TGF beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF beta superfamily that share a characteristic cystine knot structure. TGF beta 1, 2 and 3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial mesenchymal transition. Each TGF beta isoform has some non redundant functions; for TGF beta 1, mice with targeted deletion show defects in hematopoiesis and endothelial differentiation, and die of overwhelming inflammation. Human TGF beta 1 cDNA encodes a 390 amino acid (aa) precursor that contains a 29 aa signal peptide and a 361 aa proprotein. A furin-like convertase processes the proprotein to generate an N terminal 249 aa latency associated peptide (LAP) and a C terminal 112 aa mature TGF beta 1. Disulfide-linked homodimers of LAP and TGF beta 1 remain non covalently associated after secretion, forming the small latent TGF beta 1 complex. Covalent linkage of LAP to one of three latent TGF beta binding proteins (LTBPs) creates a large latent complex that may interact with the extracellular matrix. TGF beta is activated from latency by pathways that include actions of the protease plasmin, matrix metalloproteases, thrombospondin 1 and a subset of integrins. Mature human TGF beta 1 shares 100% aa identity with pig, dog and cow TGF beta 1, and 99% aa identity with mouse, rat and horse TGF beta 1. It demonstrates cross species activity. TGF beta 1 signaling begins with high affinity binding to a type II ser/thr kinase receptor termed TGF beta RII. This receptor then phosphorylates and activates a second ser/thr kinase receptor, TGF beta RI (also called activin receptor like kinase (ALK) 5), or alternatively, ALK 1. This complex phosphorylates and activates Smad proteins that regulate transcription. Contributions of the accessory receptors betaglycan (also known as TGF beta RIII) and endoglin, or use of Smad-independent signaling pathways, allow for disparate actions observed in response to TGF beta in different contexts.
生物活性	Immobilized Recombinant Human TGF-beta 1 Monomer protein (rp176238) at 1.0 μg/mL can bind Recombinant TGF beta 1 Antibody (Ab130853) with the EC50 of 5 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	279-390 aa
序列	MGSSHHHHHHSSGLVPRGSHMALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P01137
来源	重组表达
预测分子量	15.1 kDa
SDS-PAGE	13.9 kDa, under reducing conditions; 13.7 & 28.8 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 4mM HCl, pH 3.0
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile 4mM HCl, pH 3.0. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human TGF-beta 1 Monomer Protein (rp176238)

Recombinant Human TGF-beta 1 Monomer Protein (rp176238) - ELISA
Immobilized Recombinant Human TGF-beta 1 Monomer Protein (rp176238) at 1.0 μg/mL can bind Recombinant TGF beta 1 Antibody (Ab130853) with the EC ₅₀ of 5 ng/mL.

SDS-PAGE: Recombinant Human TGF-beta 1 Monomer Protein (rp176238)

Recombinant Human TGF-beta 1 Monomer Protein (rp176238) - SDS-PAGE
3 μg/lane of Recombinant Human TGF-beta 1 Monomer Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 13.9 kDa under reducing conditions and 13.7 & 28.8 kDa under non-reducing conditions.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0809255	分析证书	rp176238
ZJ24F0809256	分析证书	rp176238
ZJ24F0809257	分析证书	rp176238
ZJ24F0809258	分析证书	rp176238

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Recombinant Human TGF-beta 1 Monomer Protein

库存信息

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基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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