Recombinant Bovine CD4 Protein

COA

级别和纯度:

≥90%(SDS-PAGE)

表达系统: HEK293
Accession #: F1MJK4
蛋白标签: C-His
生物活性: Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells.The ED50for this effect is 0.1-0.5 μg/mL after a 1 hour incubation at37 °C.Optimal dilutions should be determined by each laboratory for each application.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp175916-10μg	10μg	现货
rp175916-50μg	50μg	现货
rp175916-100μg	100μg	现货
rp175916-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD4

Accession#: F1MJK4 (1) Bovine CD4 (1) CD40 (40) Gene ID: 407098 (1)

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基本描述

产品名称	Recombinant Bovine CD4 Protein
别名	重组牛CD4蛋白 \| 重组牛 CD4 蛋白
英文别名	T-cell surface glycoprotein CD4 \| T-cell surface antigen T4/Leu-3 \| CD4
规格或纯度	无动物源, 无载体, Azide Free, 生物活性, ActiBioPure™, High performance, ≥90%(SDS-PAGE)
产品介绍	Purity:> 90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature feline CD4 consists of a 388 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, feline CD4 shares 70%, 58%, 50%, and 48% aa sequence identity with canine, human, mouse and rat CD4, respectively. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity. CD4 is reexpressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a coreceptor for the gp120 surface glycoprotein of HIV-1.
生物活性	Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells.The ED50for this effect is 0.1-0.5 μg/mL after a 1 hour incubation at37 °C.Optimal dilutions should be determined by each laboratory for each application.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	牛(Bovine)
氨基酸	1-394 aa
序列	MGPGTSLRHLFLVLQLAMLPAGTQGKTVVLGEAGDKAELPCQASQKKNMVFSWKDSSQSNILGKRGLFFYKGTTELSHRVESKKNLWDQGSFPLIIKNLQVTDSGTYTCEVDKKTLEVELQVFRLTASSDTHVLLGQSLTLTLESPSGSNPSVQWKGPGDNNKRDVKSLSLAQVGLQDSGTWTCTISQSQQTLEIKIPIVVLAFQKAPETVYVKEGEQAEFSFPLTFEYENLSGELTWQLANGDSSSQSWVTFTVKNREVKVNKIHNDPKLLVGEKLPLRLTLPRTLPQHAGSGTLTLDLTKGKLQQKVKLVVMKVTKSPNSLTCEVLGPSPPKLTLNLKMGNQSMKGSNQPKLVTQPEPQAGMWQCLLSDNGKVLLEAKIEVLPSEFTQAWPKHHHHHH
纯度	>90% (SDS-PAGE)
蛋白标签	C-His
N-末端序列	Ala22
无载体	Yes
无动物源	Yes
Accession #	F1MJK4
来源	重组表达
预测分子量	41.6 kDa
SDS-PAGE	38.8-53.9 kDa, under reducing conditions; 37.1-51.0 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Bovine CD4 Protein (rp175916)

Recombinant Bovine CD4 Protein (rp175916) - SDS-PAGE
3 μg/lane of Recombinant Bovine CD4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 38.8-53.9 kDa under reducing conditions and 37.1-51.0 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0505229	分析证书	rp175916
ZJ24F0505230	分析证书	rp175916
ZJ24F0505231	分析证书	rp175916

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