Recombinant Human Leptin Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: Q6NT58
蛋白标签: No tag
生物活性: Immobilized Recombinant Human Leptin Receptor Protein (rp184128) at 2.0 μg/mL can bind Recombinant Human Leptin Protein (rp170412) with the EC50 of 6.6 ng/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货	价格	数量
rp170412-100μg	100μg	现货
rp170412-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: LEP

Accession#: Q6NT58 (1) Gene ID:3952 (2) Lep (4) Leptin (4)

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基本描述

产品名称	Recombinant Human Leptin Protein
别名	重组人Leptin蛋白 \| 重组人类瘦素蛋白
英文别名	FLJ94114 \| LEP \| leptin (murine obesity homolog) \| leptin (obesity homolog, mouse) \| Leptin \| OB \| Obese protein \| obese, mouse, homolog of \| Obesity factor \| OBOBS
规格或纯度	无载体, 生物活性, ActiBioPure™, ≥95%(SDS-PAGE)
产品介绍	Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Leptin is a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the sythesis of Leptin have been found to be obese, diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse and rat cells. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid residue signal sequence that is cleaved to yield the 146 amino acid residue mature protein. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. A high-affinity receptor for Leptin (OB-R) with homology to gp130 and the G-CSF receptor has been cloned. OB-R mRNA has been shown to be expressed in the choroid plexus and in the hypothalamus. OB-R has also been identified as an isoform of B219, a sequence that is expressed in at least four isoforms in very primitive hematopoietic cell populations and in a variety of lymphohematopoietic cell lines. The roles of leptin in body weight regulation, hematopoiesis and reproduction continue to be investigated.
生物活性	Immobilized Recombinant Human Leptin Receptor Protein (rp184128) at 2.0 μg/mL can bind Recombinant Human Leptin Protein (rp170412) with the EC50 of 6.6 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	22-167 aa
序列	VPIQKVQDDTKTLIKTIVTRINDISHTQSVSSKQKVTGLDFIPGLHPILTLSKMDQTLAVYQQILTSMPSRNVIQISNDLENLRDLLHVLAFSKSCHLPWASGLETLDSLGGVLEASGYSTEVVALSRLQGSLQDMLWQLDLSPGC
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	Q6NT58
来源	重组表达
预测分子量	16.0 kDa
SDS-PAGE	12.6 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human Leptin Protein (rp170412)

Recombinant Human Leptin Protein (rp170412) - SDS-PAGE
3 μg/lane of Recombinant Human Leptin Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 12.6 kDa under reducing conditions.

Binding Activity: Recombinant Human Leptin Protein (rp170412)

Recombinant Human Leptin Protein (rp170412) - Binding Activity
Immobilized Recombinant Human Leptin Receptor Protein (rp184128) at 2.0 μg/mL can bind Recombinant Human Leptin Protein (rp170412) with the EC50 of 6.6 ng/mL.

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批号(Lot Number)	证书类型	货号
ZJ24F0606780	分析证书	rp170412

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