Recombinant Human UbcH5a/UBE2D1 Protein

COA

级别和纯度:

无载体

无叠氮钠

≥90%(SDS-PAGE)

表达系统: E. coli
Accession #: P51668
蛋白标签: N-His
生物活性: Testing in progress
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp170402-10μg	10μg	现货
rp170402-50μg	50μg	现货
rp170402-100μg	100μg	现货
rp170402-1mg	1mg	现货

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点击查看蛋白信息: UBE2D1

Accession#:P51668 (2) Gene ID:7321 (2) UbcH5a (1) UBE2D1 (2)

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基本描述

产品名称	Recombinant Human UbcH5a/UBE2D1 Protein
别名	重组人 UbcH5a/UBE2D1蛋白 \| 重组人 UbcH5a/UBE2D1 蛋白
英文别名	E2 ubiquitin-conjugating enzyme D1 \| Stimulator of Fe transport (SFT) \| UBC4/5 homolog \| UbcH5 \| (E3-independent) E2 ubiquitin-conjugating enzyme D1 (EC:2.3.2.24) \| EC:2.3.2.24 \| Ubiquitin-conjugating enzyme E2 D1 \| (E3-independent) E2 ubiquitin-conjugati
规格或纯度	无载体, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively. Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins. UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53. Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1. Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses.
生物活性	Testing in progress
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	1-147 aa
序列	MHHHHHHALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P51668
来源	重组表达
预测分子量	17.4 kDa
SDS-PAGE	15.3 kDa, under reducing conditions; 15.3 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human UbcH5a/UBE2D1 Protein (rp170402)

Recombinant Human UbcH5a/UBE2D1 Protein (rp170402) - SDS-PAGE
Recombinant Human UbcH5a/UBE2D1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.3 kDa.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ23F1101748	分析证书	rp170402
ZJ23F1101749	分析证书	rp170402
ZJ23F1101750	分析证书	rp170402
ZJ23F1101751	分析证书	rp170402

技术文档和文章

引用文献

1. Chen H, Wu G, Gao S, Guo R, Zhao Z, Yuan H, Liu S, Wu J, Lu X, Yuan X, Yu Z, Zu X, Xie N, Yang N, Hu Z, Sun Q, Zhang W.. (2017) Discovery of Potent Small-Molecule Inhibitors of Ubiquitin-Conjugating Enzyme UbcH5c from α-Santonin Derivatives.. J Med Chem, 60 (16): (6828-6852). [PMID:28696694] [10.1021/acs.jmedchem.6b01829]
2. Scheffner, M M, Huibregtse, J M JM and Howley, P M PM.. (1994) Identification of a human ubiquitin-conjugating enzyme that mediates the E6-AP-dependent ubiquitination of p53.. Proceedings of the National Academy of Sciences of the United States of America, (13): [PMID:8090726]
3. Gutierrez, J A JA, Yu, J J, Rivera, S S and Wessling-Resnick, M M.. (1997) Functional expression cloning and characterization of SFT, a stimulator of Fe transport.. The Journal of cell biology, (17): [PMID:9362508]
4. Matsuzawa, S I SI and Reed, J C JC.. (2001) Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.. Molecular cell, [PMID:11389839]
5. Gehrke, Sven G SG and 6 more authors.. (2003) UbcH5A, a member of human E2 ubiquitin-conjugating enzymes, is closely related to SFT, a stimulator of iron transport, and is up-regulated in hereditary hemochromatosis.. Blood, (15): [PMID:12480712]
6. Subramaniam, V V and 8 more authors.. (2003) The RING-H2 protein RNF11 is overexpressed in breast cancer and is a target of Smurf2 E3 ligase.. British journal of cancer, (20): [PMID:14562029]
7. Deloukas, P P and 135 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 10.. Nature, (27): [PMID:15164054]
8. Windheim, Mark M, Peggie, Mark M and Cohen, Philip P.. (2008) Two different classes of E2 ubiquitin-conjugating enzymes are required for the mono-ubiquitination of proteins and elongation by polyubiquitin chains with a specific topology.. The Biochemical journal, (1): [PMID:18042044]
9. Grou, Cláudia P CP and 8 more authors.. (2008) Members of the E2D (UbcH5) family mediate the ubiquitination of the conserved cysteine of Pex5p, the peroxisomal import receptor.. The Journal of biological chemistry, (23): [PMID:18359941]
10. Espinosa, Alexander A and 5 more authors.. (2008) The autoantigen Ro52 is an E3 ligase resident in the cytoplasm but enters the nucleus upon cellular exposure to nitric oxide.. Experimental cell research, (10): [PMID:18845142]
11. Pabarcus, Michael K MK and 5 more authors.. (2009) CYP3A4 ubiquitination by gp78 (the tumor autocrine motility factor receptor, AMFR) and CHIP E3 ligases.. Archives of biochemistry and biophysics, (1): [PMID:19103148]
12. Zeng, Wenwen W, Xu, Ming M, Liu, Siqi S, Sun, Lijun L and Chen, Zhijian J ZJ.. (2009) Key role of Ubc5 and lysine-63 polyubiquitination in viral activation of IRF3.. Molecular cell, (23): [PMID:19854139]
13. David, Yael Y, Ziv, Tamar T, Admon, Arie A and Navon, Ami A.. (2010) The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.. The Journal of biological chemistry, (19): [PMID:20061386]
14. Zhang, Li L and 7 more authors.. (2011) The IDOL-UBE2D complex mediates sterol-dependent degradation of the LDL receptor.. Genes & development, (15): [PMID:21685362]
15. Sheng, Yi Y and 13 more authors.. (2012) A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.. Molecular & cellular proteomics : MCP, [PMID:22496338]

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