Recombinant Human p53 Protein

COA

级别和纯度:

无载体

无叠氮钠

≥90%(SDS-PAGE)

表达系统: E. coli
Accession #: P04637
蛋白标签: N-His

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp170134-10μg	10μg	现货
rp170134-100μg	100μg	现货
rp170134-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: TP53

Accession#:P04637 (5) Gene ID:7157 (5) p53 (5) TP53 (5)

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基本描述

产品名称	Recombinant Human p53 Protein
别名	重组人p53蛋白 \| 重组人 p53 蛋白
英文别名	Antigen NY-CO-13 \| BCC7 \| Cellular tumor antigen p53 \| FLJ92943 \| LFS1 \| Mutant tumor protein 53 \| p53 \| p53 tumor suppressor \| P53_HUMAN \| Phosphoprotein p53 \| Tp53 \| Transformation related protein 53 \| TRP53 \| tumor antigen p53 \| Tumor protein 53 \| Tumo
规格或纯度	Carrier Free, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： p53 is well known for its key role as a tumor suppressor protein. It is 393 amino acids (aa) in length with a predicted molecular weight of 44 kDa. It belongs to the p53 family that also includes p63 and p73. Structurally, p53 is characterized by an N-terminal transactivation domain, central DNA-binding and oligomerization domains, and a C-terminal regulatory domain. It is thought to exist as a homotetramer, and it exhibits approximately 72% and 76% aa identity with its mouse and rat orthologs, respectively. Mutations in the p53 gene are one of the most frequent genomic events accompanying oncogenic transformation. p53 responds to signals such as DNA damage or cell stress primarily through its actions as a transcription factor. Among its gene targets are a range factors that promote DNA repair mechanisms or apoptosis, including cell cycle regulatory proteins and members the Bcl-2 family. Because of its critical role in genomic homeostasis, p53 activities are tightly regulated by a network of protein-protein interactions, microRNAs, and a range of post-translational modifications, including phosphorylation, acetylation, methylation, and ubiquitination. A widely studied regulator is Murine Double Minute 2 (MDM2). MDM2 is known to suppress p53 activity through direct binding or through its actions as a Ubiquitin ligase (E3) that catalyzes p53 ubiquitination and proteasome-mediated degradation.
表达系统	E. coli
种属	人(Human)
氨基酸	1-393 aa
序列	MGSSHHHHHHSSGLVPRGSHMEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD
纯度	>90% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P04637
来源	重组表达
预测分子量	45.8 kDa
SDS-PAGE	55.4 kDa, under reducing conditions; 52.5 and 122.4 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM Tris, 500mM NaCl, 300mM Imidazole, 8M Urea, pH8.0
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human p53 Protein (rp170134)

Recombinant Human p53 Protein (rp170134) - SDS-PAGE
2 μg/lane of Recombinant Human p53 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 55.4 kDa under reducing conditions and 52.5 & 122.4 kDa under non-reducing conditions.

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批号(Lot Number)	证书类型	货号
ZJ24F0303510	分析证书	rp170134
ZJ24F0303511	分析证书	rp170134

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