Recombinant Human FGF-21 Protein

COA

级别和纯度:

无载体

无叠氮钠

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: Q9NSA1
蛋白标签: N-His
生物活性: Testing in progress
内毒素水平: <1.0 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp169669-10μg	10μg	现货
rp169669-50μg	50μg	现货
rp169669-100μg	100μg	现货
rp169669-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: FGF21

Accession#: Q9NSA1 (1) FGF-21 (1) Fgf21 (3) Gene ID: 26291 (1)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Human FGF-21 Protein
别名	重组人FGF-21蛋白 \| 重组人 FGF-21 蛋白
英文别名	FGF 21 \| FGF-21 \| Fgf21 \| FGF21_HUMAN \| FGFL \| Fibroblast growth factor 21 \| PRO10196 \| UNQ3115
规格或纯度	无载体, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: Fibroblast growth factor 21 (FGF-21) is a member of the FGF gene family, which currently contains 22 human members. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure. Unlike other FGF subfamilies, FGF-19 subfamily members apparently exhibit poor binding to ECM, resulting in highly diffusible molecules. The c-DNA for FGF-21 predicts a 209 aa polypeptide that contains a 28 aa signal sequence and a 181 aa mature region. Notably, FGF-21, as well as FGF-19 show limited binding to heparin. One potential alternate splice form has been reported. It shows a 43 aa substitution for the C-terminal 12 aa of the standard form. Mature human FGF-21 shows 81% aa identity to mouse FGF-21, and is known to be active on mouse cells. The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism, all three are induced by a nuclear receptor heterodimer that includes RXR, and all three utilize Klotho family members for signal transduction. FGF-21 is produced by hepatocytes in response to free fatty acid (FFA) stimulation of a PPARa/RXR dimeric complex. This situation occurs clinically during starvation, or following the ingestion of a high-fat/low-carbohydrate diet. Upon FGF-21 secretion, white adipose tissue is induced to release FFAs from triglyceride stores. Once FFAs reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies (acetoacetate and beta -hydroxybutyrate), released, and transported to peripheral tissues for TCA processing and energy generation.
生物活性	Testing in progress
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	29-209 aa
序列	MGSSHHHHHHSSGLVPRGSHMMHPIPDSSPLLQFGGQVRQRYLYTDDAQQTEAHLEIREDGTVGGAADQSPESLLQLKALKPGVIQILGVKTSRFLCQRPDGALYGSLHFDPEACSFRELLLEDGYNVYQSEAHGLPLHLPGNKSPHRDPAPRGPARFLPLPGLPPALPEPPGILAPQPPDVGSSDPLSMVGPSQGRSPSYAS
纯度	>95% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	Q9NSA1
来源	重组表达
预测分子量	21.8 kDa
SDS-PAGE	26.2 kDa, under reducing conditions；25.3 kDa, under non-reducing condition

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human FGF-21 Protein (rp169669)

Recombinant Human FGF-21 Protein (rp169669) - SDS-PAGE
3 μg/lane of Recombinant Human FGF-21 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 26.2 kDa under reducing conditions and 25.3 kDa under non-reducing conditions.