Recombinant Human mTOR Protein

COA

级别和纯度:

无载体

无叠氮钠

≥90%(SDS-PAGE)

表达系统: E. coli
Accession #: P42345
蛋白标签: N-His
内毒素水平: <1.0 EU/μg

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp169612-10μg	10μg	现货
rp169612-100μg	100μg	现货
rp169612-1mg	1mg	期货

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点击查看蛋白信息: MTOR

Accession#:P42345 (2) Gene ID:2475 (2) MTOR (146)

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基本描述

产品名称	Recombinant Human mTOR Protein
别名	重组人mTOR蛋白 \| 重组人类 mTOR 蛋白
英文别名	dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) \| FK506 binding protein 12 rapamycin associated protein 1 \| FK506 binding protein 12 rapamycin associated protein 2 \| FK506 binding protein 12 rapamycin complex associated protein 1 \| FK5
规格或纯度	Carrier Free, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	2226-2529 aa
序列	MGSSHHHHHHSSGLVPRGSAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLI
纯度	>90% (SDS-PAGE)
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P42345
来源	重组表达
预测分子量	36.6 kDa
SDS-PAGE	37.5 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 20mM Tris, 400mM NaCl, 50mM Imidazole, PH 8.0
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human mTOR Protein (rp169612)

Recombinant Human mTOR Protein (rp169612) - SDS-PAGE
4 μg/lane of Recombinant Human mTOR Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 37.5 kDa under reducing conditions.

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批号(Lot Number)	证书类型	货号
ZJ24F0404982	分析证书	rp169612
ZJ24F0404983	分析证书	rp169612

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