货号 (SKU)

包装规格

是否现货

价格

数量

rp169548-10μg

10μg

现货

rp169548-100μg

100μg

现货

rp169548-1mg

1mg

期货

基本描述

产品名称	Recombinant Human Kallikrein 3/PSA Protein
别名	重组人前列腺特异性抗原/激肽释放酶-3 \| 重组人Kallikrein 3/PSA蛋白 \| 重组人激肽释放酶 3/PSA 蛋白
英文别名	EC 3.4.21.77 \| Gamma-seminoprotein \| Kallikrein-3 \| P-30 antigen \| PSA \| Semenogelase \| Seminin \| APS seminin \| EC 3.4.21 \| EC 3.4.21.77 \| Gamma-seminoprotein \| hK3 \| Kallikrein 3 \| kallikrein 3, (prostate specific antigen) \| Kallikrein-3 \| kallikrein-rel
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： KLK3 (Kallikrein Related Peptidase 3) is a Protein Coding gene. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease that is synthesized in the epithelial cells of the prostate gland and is present in seminal plasma. KLK3, also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases. KLK3 contains one peptidase S1 domain. KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.
生物活性	Measured by its binding ability in a functional ELISA. Immobilized human Kallikrein 3/PSA (rp169548) at 1 μg/mL can bind PSA Mouse mAb (Ab005494) with the EC50 of 78.61 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	18-261 aa
序列	APLILSRIVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANPHHHHHHHHHH
纯度	>95% (SDS-PAGE)
蛋白标签	C-His
N-末端序列	Ala18
无载体	Yes
无动物源	Yes
Accession #	P07288
来源	重组表达
预测分子量	28.2 kDa
SDS-PAGE	33.8 kDa, under reducing conditions; 30.3 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.1 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human Kallikrein 3/PSA Protein (rp169548)

Recombinant Human Kallikrein 3/PSA Protein (rp169548) - ELISA
Measured by its binding ability in a functional ELISA. Immobilized human Kallikrein 3/PSA (rp169548) at 1 μg/mL can bind PSA Mouse mAb (Ab005494) with the EC₅₀ of 78.61 ng/mL.

SDS-PAGE: Recombinant Human Kallikrein 3/PSA Protein (rp169548)

Recombinant Human Kallikrein 3/PSA Protein (rp169548) - SDS-PAGE
3 μg/lane of Recombinant Human Kallikrein 3/PSA Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 33.8 kDa under reducing conditions and 30.3 kDa under non-reducing conditions.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102692	分析证书	rp169548
ZJ24F0102693	分析证书	rp169548
ZJ25F0420241	分析证书	rp169548
ZJ25F0420240	分析证书	rp169548

技术文档和文章

阿拉丁®靶点蛋白

Categories: 技术文章

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药物开发信号通路细胞因子靶点蛋白药物靶点受体免疫检查点细胞表面标记物

引用文献

1. Kostova MB, Rosen DM, Chen Y, Mease RC, Denmeade SR. (2013) Structural optimization, biological evaluation, and application of peptidomimetic prostate specific antigen inhibitors.. J Med Chem, 56 (11): (4224-35). [PMID:23692593]
2. De Vita E, Schüler P, Lovell S, Lohbeck J, Kullmann S, Rabinovich E, Sananes A, Heßling B, Hamon V, Papo N et al.. (2018) Depsipeptides Featuring a Neutral P1 Are Potent Inhibitors of Kallikrein-Related Peptidase 6 with On-Target Cellular Activity.. J Med Chem, 61 (19): (8859-8874). [PMID:30212625]
3. LeBeau AM, Banerjee SR, Pomper MG, Mease RC, Denmeade SR.. (2009) Optimization of peptide-based inhibitors of prostate-specific antigen (PSA) as targeted imaging agents for prostate cancer.. Bioorg Med Chem, 17 (14): (4888-4893). [PMID:19541487] [10.1016/j.bmc.2009.06.012]
4. Santos JA, Kondo MY, Freitas RF, dos Santos MH, Ramalho TC, Assis DM, Juliano L, Juliano MA, Puzer L.. (2016) The natural flavone fukugetin as a mixed-type inhibitor for human tissue kallikreins.. Bioorg Med Chem Lett, 26 (5): (1485-1489). [PMID:26848109] [10.1016/j.bmcl.2016.01.039]
5. Wu X, Hu L.. (2016) Design and synthesis of peptide conjugates of phosphoramide mustard as prodrugs activated by prostate-specific antigen.. Bioorg Med Chem, 24 (12): (2697-2706). [PMID:27156193] [10.1016/j.bmc.2016.04.035]
6. Akinboye ES, Rosen MD, Bakare O, Denmeade SR.. (2017) Anticancer activities of emetine prodrugs that are proteolytically activated by the prostate specific antigen (PSA) and evaluation of in vivo toxicity of emetine derivatives.. Bioorg Med Chem, 25 (24): (6707-6717). [PMID:29153549] [10.1016/j.bmc.2017.11.015]
7. Bakthavatsalam S, Wiangnak P, George DJ, Zhang T, Franz KJ.. (2020) Dithiocarbamate prodrugs activated by prostate specific antigen to target prostate cancer.. Bioorg Med Chem Lett, 30 (11): (127148-127148). [PMID:32253061] [10.1016/j.bmcl.2020.127148]
8. Tang Q,Fu W,Zhang M,Wang E,Shan L,Chai X,Pang J,Wang X,Xu X,Xu L,Li D,Sheng R,Hou T. (2020) Novel androgen receptor antagonist identified by structure-based virtual screening, structural optimization, and biological evaluation.. Eur J Med Chem, 192 (112156-112156). [PMID:32114360] [10.1016/j.ejmech.2020.112156]
9. España, F F and 5 more authors.. (1991) Functionally active protein C inhibitor/plasminogen activator inhibitor-3 (PCI/PAI-3) is secreted in seminal vesicles, occurs at high concentrations in human seminal plasma and complexes with prostate-specific antigen.. Thrombosis research, (1): [PMID:1725227]
10. Watt, K W KW, Lee, P J PJ, M'Timkulu, T T, Chan, W P WP and Loor, R R.. (1986) Human prostate-specific antigen: structural and functional similarity with serine proteases.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2422647]
11. Lundwall, A A and Lilja, H H.. (1987) Molecular cloning of human prostate specific antigen cDNA.. FEBS letters, (20): [PMID:2436946]
12. Schulz, P P and 5 more authors.. (1988) Sequence of a cDNA clone encompassing the complete mature human prostate specific antigen (PSA) and an unspliced leader sequence.. Nucleic acids research, (11): [PMID:2456523]
13. Riegman, P H PH, Vlietstra, R J RJ, van der Korput, J A JA, Romijn, J C JC and Trapman, J J.. (1989) Characterization of the prostate-specific antigen gene: a novel human kallikrein-like gene.. Biochemical and biophysical research communications, (28): [PMID:2466464]
14. Digby, M M, Zhang, X Y XY and Richards, R I RI.. (1989) Human prostate specific antigen (PSA) gene: structure and linkage to the kallikrein-like gene, hGK-1.. Nucleic acids research, (11): [PMID:2467258]
15. Klobeck, H G HG, Combriato, G G, Schulz, P P, Arbusow, V V and Fittler, F F.. (1989) Genomic sequence of human prostate specific antigen (PSA).. Nucleic acids research, (25): [PMID:2471958]
16. and Lundwall, A A.. (1989) Characterization of the gene for prostate-specific antigen, a human glandular kallikrein.. Biochemical and biophysical research communications, (30): [PMID:2472789]
17. Schaller, J J and 5 more authors.. (1987) Isolation, characterization and amino-acid sequence of gamma-seminoprotein, a glycoprotein from human seminal plasma.. European journal of biochemistry, (30): [PMID:3691515]
18. Monne, M M, Croce, C M CM, Yu, H H and Diamandis, E P EP.. (1994) Molecular characterization of prostate-specific antigen messenger RNA expressed in breast tumors.. Cancer research, (15): [PMID:7527295]
19. Villoutreix, B O BO, Getzoff, E D ED and Griffin, J H JH.. (1994) A structural model for the prostate disease marker, human prostate-specific antigen.. Protein science : a publication of the Protein Society, [PMID:7535613]
20. Baffa, R R, Moreno, J G JG, Monne, M M, Veronese, M L ML and Gomella, L G LG.. (1996) A comparative analysis of prostate-specific antigen gene sequence in benign and malignant prostate tissue.. Urology, [PMID:8677566]
21. Coombs, G S GS and 8 more authors.. (1998) Substrate specificity of prostate-specific antigen (PSA).. Chemistry & biology, [PMID:9751643]
22. Gan, L L and 8 more authors.. (2000) Sequencing and expression analysis of the serine protease gene cluster located in chromosome 19q13 region.. Gene, (17): [PMID:11054574]
23. Ménez, Renée R and 6 more authors.. (2008) Crystal structure of a ternary complex between human prostate-specific antigen, its substrate acyl intermediate and an activating antibody.. Journal of molecular biology, (29): [PMID:18187150]
24. Végvári, Akos A and 6 more authors.. (2013) Identification of a novel proteoform of prostate specific antigen (SNP-L132I) in clinical samples by multiple reaction monitoring.. Molecular & cellular proteomics : MCP, [PMID:23842001]
25. Henttu, P P and Vihko, P P.. (1989) cDNA coding for the entire human prostate specific antigen shows high homologies to the human tissue kallikrein genes.. Biochemical and biophysical research communications, (28): [PMID:2470373]

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体积（添加到小瓶中）

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所需的复溶浓度

Recombinant Human Kallikrein 3/PSA Protein

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器