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Recombinant Human GFR alpha-like Protein

Recombinant Human GFR alpha-like Protein

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q6UXV0
蛋白标签: C-His
生物活性: Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human GDF15, hFc Tag with the EC50 of 9.4ng/ml.
内毒素水平: <0.1 EU/μg

有货

库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp167646-10μg	10μg	现货
rp167646-50μg	50μg	现货
rp167646-100μg	100μg	现货
rp167646-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: GFRAL

Accession#:Q6UXV0 (2) Gene ID:389400 (2) GFR alpha-like (1) GFRAL (2)

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基本描述

产品名称	Recombinant Human GFR alpha-like Protein
别名	重组人GFRAL蛋白 \| 重组人 GFR alpha 样蛋白
英文别名	GFR alpha like \| GFR alpha-like \| GFRAL \| GRAL \| UNQ9356 \| C6orf144 \| bA360D14.1
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% (SDS-PAGE) Endotoxin level ＜0.1 EU/μg Function GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain. Over the extracellular domain, human GFRAL shares 72% and 71% identity with mouse and rat GFRAL respectively. GFRAL is expressed on both fetal and adult hindbrain neurons of the CNS, and would appear to function as an anti-apoptotic molecule during neuronal stress. GFRAL is a functional receptor for GDF-15, facilitating weight-loss functions of the protein through c-Ret downstream signaling. GFRAL and GDF-15 signaling is implicated in diet-based obesity and insulin resistance.
生物活性	Immobilized Human GFRAL, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Human GDF15, hFc Tag with the EC50 of 9.4ng/ml.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	19-351 aa
序列	SQTNNCTYLREQCLRDANGCKHAWRVMEDACNDSDPGDPCKMRNSSYCNLSIQYLVESNFQFKECLCTDDFYCTVNKLLGKKCINKSDNVKEDKFKWNLTTRSHHGFKGMWSCLEVAEACVGDVVCNAQLASYLKACSANGNPCDLKQCQAAIRFFYQNIPFNIAQMLAFCDCAQSDIPCQQSKEALHSKTCAVNMVPPPTCLSVIRSCQNDELCRRHYRTFQSKCWQRVTRKCHEDENCISTLSKQDLTCSGSDDCKAAYIDILGTVLQVQCTCRTITQSEESLCKIFQHMLHRKSCFNYPTLSNVKGMALYTRKHANKITLTGFHSPFNGEHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	Q6UXV0
来源	重组表达
预测分子量	39 kDa
SDS-PAGE	40.0 - 50.0 kDa, under reducing conditions; 40.0 - 50.0 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human GFR alpha-like Protein (rp167646)

Recombinant Human GFR alpha-like Protein (rp167646) - Protein Bioactivity
Immobilized Recombinant Human GFR alpha-like Protein (rp167646) at 0.5 μg/mL can bind Human GDF15 with the EC ₅₀ of 8.5 ng/mL.

SDS-PAGE: Recombinant Human GFR alpha-like Protein (rp167646)

Recombinant Human GFR alpha-like Protein (rp167646) - SDS-PAGE
3 μg/lane of Recombinant Human GFR alpha-like Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 40.0 - 50.0 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0607409	分析证书	rp167646
ZJ24F0607407	分析证书	rp167646
ZJ24F0607408	分析证书	rp167646

技术文档和文章

引用文献

1. Mullican SE, Lin-Schmidt X, Chin CN, Chavez JA, Furman JL, Armstrong AA, Beck SC, South VJ, Dinh TQ, Cash-Mason TD et al.. (2017) GFRAL is the receptor for GDF15 and the ligand promotes weight loss in mice and nonhuman primates.. Nat Med, 23 (10): (1150-1157). [PMID:28846097]
2. Emmerson PJ, Wang F, Du Y, Liu Q, Pickard RT, Gonciarz MD, Coskun T, Hamang MJ, Sindelar DK, Ballman KK et al.. (2017) The metabolic effects of GDF15 are mediated by the orphan receptor GFRAL.. Nat Med, 23 (10): (1215-1219). [PMID:28846098]
3. Yang L, Chang CC, Sun Z, Madsen D, Zhu H, Padkjær SB, Wu X, Huang T, Hultman K, Paulsen SJ et al.. (2017) GFRAL is the receptor for GDF15 and is required for the anti-obesity effects of the ligand.. Nat Med, 23 (10): (1158-1166). [PMID:28846099]
4. Hsu, Jer-Yuan JY and 33 more authors.. (2017) Non-homeostatic body weight regulation through a brainstem-restricted receptor for GDF15.. Nature, (12): [PMID:28953886]

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