Recombinant Human IL-1β Protein

COA

级别和纯度:

无载体

生物活性

ActiBioPure™

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P01584
蛋白标签: No tag
生物活性: 1. Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL. 2. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 52.68 ng/mL. 3. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC50 of 22.81 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp156012-10μg	10μg	现货
rp156012-50μg	50μg	现货
rp156012-100μg	100μg	现货
rp156012-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL1B

Accession#:P01584 (8) Gene ID:3553 (10) IL-1β (2) IL1B (14)

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基本描述

产品名称	Recombinant Human IL-1β Protein
别名	重组人IL-1β蛋白 \| 重组人IL-1β蛋白
英文别名	IL1 beta \| IL-1 beta \| IL-1 \| IL1B \| IL-1b \| IL1-BETA \| IL-1F2 \| IL1F2IL-1 beta \| interleukin 1, beta \| interleukin-1 beta \| preinterleukin 1 beta \| pro-interleukin-1-beta \| catabolin
规格或纯度	ActiBioPure™, Bioactive, Carrier Free, ≥95%(SDS-PAGE)
产品介绍	Protein Function: IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in humans. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine. The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.
生物活性	1. Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL. 2. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC50 of 52.68 ng/mL. 3. Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC50 of 22.81 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	117-269 aa
序列	APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS
蛋白标签	No tag
无载体	Yes
无动物源	No
Accession #	P01584
来源	重组表达
预测分子量	17.4 kDa
SDS-PAGE	15.0 kDa, under reducing conditions; 15.1 kDa, under non-reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

ELISA: Recombinant Human IL-1β Protein (rp156012)

Recombinant Human IL-1β Protein (rp156012) - ELISA
Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Gevokizumab (anti-IL-1b) (Ab175856) with the EC₅₀ of 52.68 ng/mL.

ELISA: Recombinant Human IL-1β Protein (rp156012)

Recombinant Human IL-1β Protein (rp156012) - ELISA
Immobilized Recombinant Human IL-1β Protein (rp156012) at 1.0 μg/mL can bind Canakinumab (Anti-IL-1b) (Ab170512) with the EC₅₀ of 22.81 ng/mL.

SDS-PAGE: Recombinant Human IL-1β Protein (rp156012)

Recombinant Human IL-1β Protein (rp156012) - SDS-PAGE
3 μg/lane of Recombinant Human IL-1β Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 15.0 kDa under reducing conditions and 15.1 kDa under non-reducing conditions.

Binding Activity: Recombinant Human IL-1β Protein (rp156012)

Recombinant Human IL-1β Protein (rp156012) - Binding Activity
Immobilized Recombinant Human IL-1β Protein (rp156012) at 5.0 μg/mL can bind Recombinant Human IL-1 RII Protein (rp181605) with the EC50 of 176.8 ng/mL.

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找到5个结果

批号(Lot Number)	证书类型	货号
ZJ25F0927479	分析证书	rp156012
ZJ25F0927478	分析证书	rp156012
ZJ24F0707630	分析证书	rp156012
ZJ24F0707628	分析证书	rp156012
ZJ24F0707629	分析证书	rp156012

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Recombinant Human IL-1β Protein

库存信息

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基本描述

储存与运输

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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