Recombinant Human GM-CSF Protein

COA

级别和纯度:

无载体

生物活性

高性能

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P04141
蛋白标签: N-His
生物活性: Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 0.82 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp156011-10μg	10μg	现货
rp156011-50μg	50μg	现货
rp156011-100μg	100μg	现货
rp156011-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CSF2

Accession#:P04141 (11) CSF2 (15) Gene ID:1437 (11) GM-CSF (15)

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基本描述

产品名称	Recombinant Human GM-CSF Protein
别名	重组人GM-CSF蛋白 \| 重组人粒细胞巨噬细胞集落刺激因子 \| 重组人 GM-CSF 蛋白
英文别名	Molgramostim \| molgramostin \| Sargramostim \| colony stimulating factor 2 (granulocyte-macrophage) \| Colony-stimulating factor \| CSF \| CSF2 \| CSF-2 \| GMCSF \| GM-CSF \| GMCSFgranulocyte-macrophage colony-stimulating factor \| granulocyte-macrophage colony sti
规格或纯度	Carrier Free, High performance, Bioactive, ≥95%(SDS-PAGE)
产品介绍	Purity:≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： GM CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy induced neutropenia, and GM CSF transfected tumor cells are utilized as cancer vaccines. The 22 kDa glycosylated GM CSF, similar to IL 3 and IL 5, is a cytokine with a core of four bundled alpha -helices. Mature human GM CSF shares 63% 70% amino acid sequence identity with canine, feline, porcine, and rat GM CSF and 54% with mouse GM CSF. GM CSF exerts its biological effects through a heterodimeric receptor complex composed of GM CSF R alpha /CD116 and the signal transducing common beta chain (CD131) which is also a component of the high affinity receptors for IL 3 and IL 5. In addition, GM CSF binds a naturally occurring soluble form of GM CSF R alpha. Human GM CSF is active on canine and feline cells but not on murine cells.
生物活性	Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically 0.82 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	18-144 aa
序列	MGSSHHHHHHSSGLVPRGSHMAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P04141
来源	重组表达
预测分子量	16.8 kDa
SDS-PAGE	14.8 kDa, under reducing conditions; 14.8 kDa, under non-reducing condition

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human GM-CSF Protein (rp156011)

Recombinant Human GM-CSF Protein (rp156011) - Protein Bioactivity
Measured in a cell proliferation assay using TF-1 cells. The ED₅₀ for this effect is typically 0.82 ng/mL.

SDS-PAGE: Recombinant Human GM-CSF Protein (rp156011)

Recombinant Human GM-CSF Protein (rp156011) - SDS-PAGE
3 μg/lane of Recombinant Human GM-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 14.8 kDa.

ELISA: Recombinant Human GM-CSF Protein (rp156011)

Recombinant Human GM-CSF Protein (rp156011) - ELISA
Immobilized Recombinant Human GM-CSF Protein (rp156011) at 1.0 μg/mL can bind Namilumab (anti-GM-CSF) (Ab175608) with the EC50 of 62.67 ng/mL.

ELISA: Recombinant Human GM-CSF Protein (rp156011)

Recombinant Human GM-CSF Protein (rp156011) - ELISA
Immobilized Recombinant Human GM-CSF Protein (rp156011) at 1.0 μg/mL can bind Gimsilumab (anti-GM-CSF) (Ab175548) with the EC50 of 23.52 ng/mL.

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批号(Lot Number)	证书类型	货号
ZJ24F0708345	分析证书	rp156011
ZJ24F0708343	分析证书	rp156011
ZJ24F0708344	分析证书	rp156011

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Recombinant Human GM-CSF Protein

库存信息

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基本描述

储存与运输

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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溶液计算器

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