Recombinant Human IL-4 Protein

COA

级别和纯度:

无载体

生物活性

高性能

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P05112
蛋白标签: N-His
生物活性: Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL.
内毒素水平: <1.0 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp156008-10μg	10μg	现货
rp156008-50μg	50μg	现货
rp156008-100μg	100μg	现货
rp156008-1mg	1mg	现货

查看相关系列

点击查看蛋白信息: IL4

Accession#:P05112 (5) Gene ID:3565 (6) IL-4 (5) Il4 (12)

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基本描述

产品名称	Recombinant Human IL-4 Protein
别名	重组人白细胞介素-4蛋白 \| 重组人IL-4蛋白 \| 重组人 IL-4 蛋白
英文别名	IL4E12 \| interleukin 4 \| interleukin-4 \| Lymphocyte stimulatory factor 1 \| MGC79402 \| pitrakinra \| B cell growth factor 1 \| BCDF \| B-cell stimulatory factor 1 \| BCGF1 \| BCGF-1 \| binetrakin \| BSF1 \| BSF-1 \| IL4 \| IL-4 \| IL-4B_cell stimulatory factor 1
规格或纯度	Carrier Free, High performance, Bioactive, ≥95%(SDS-PAGE)
产品介绍	Purity: ≥95%, by SDS-PAGE visualized with Coomassie® Blue Staining Description: Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure. Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39%, and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma. Due to its role in the differentiation of certain immune cell types, IL-4 is commonly used in combination with other growth factors to transform induced pluripotent stem cells into dendritic cells in high numbers. These dendritic cells can then be used for research or clinical applications to improve disease modeling, for screening and cell therapies. Study of IL-4 signaling has led to the development of monoclonal antibodies that can block the signaling pathway at various steps to mitigate the inflammatory response in certain autoimmune diseases. While IL-4 has the capacity to improve immune functions, treatments involving IL-4 have not been utilized due to the dangerous side effects that may result from IL-4 signaling in non-immune cells. Blockade of IL-4 signaling also has been studied as a therapeutic target to suppress inflammation in the tumor microenvironment. Use of IL-4 suppressors can also improve the efficacy of anti-tumor immunotherapies, as blocking IL-4 enhances the activity of tumor-specific T lymphocytes.
生物活性	Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	25-153 aa
序列	MGSSHHHHHHSSGLVPRGSHMHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS
蛋白标签	N-His
无载体	Yes
无动物源	No
Accession #	P05112
来源	重组表达
预测分子量	17.2 kDa
SDS-PAGE	15.5 kDa, under reducing condition

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH7.4
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20°C 下可稳定保存 1 年。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-4 Protein (rp156008)

Recombinant Human IL-4 Protein (rp156008) - Protein Bioactivity
Recombinant Human IL‑4 Protein (rp156008) stimulates cell proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.31 ng/mL.

SDS-PAGE: Recombinant Human IL-4 Protein (rp156008)

Recombinant Human IL-4 Protein (rp156008) - SDS-PAGE
3 μg/lane of Recombinant Human IL-4 Protein was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing a band at 15.5 kDa under reducing conditions.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0506232	分析证书	rp156008
ZJ24F0506233	分析证书	rp156008
ZJ24F0506234	分析证书	rp156008
ZJ24F0506235	分析证书	rp156008

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Recombinant Human IL-4 Protein

库存信息

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基本描述

储存与运输

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质检证书（CoA,COO,BSE/TSE 和分析图谱)

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