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Recombinant Human KGF Protein

  • 表达系统: 水稻(Oryza sativa)
  • Accession #: P21781.1
  • 蛋白标签: No tag
  • 生物活性: Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <10 ng/mL.
  • 内毒素水平: <0.1 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp155960-10μg
10μg 现货 Stock Image
rp155960-50μg
50μg 现货 Stock Image
rp155960-100µg
100μg 现货 Stock Image
rp155960-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant Human KGF Protein
别名 植物源重组人角质细胞生长因子 | 重组人KGF蛋白
英文别名 FGF7 | FGF-7 | fibroblast growth factor 7HBGF-7 | HBGF7 | HBGF-7 | Heparin-binding growth factor 7 | keratinocyte growth factor | KGF | KGFfibroblast growth factor 7 (keratinocyte growth factor)
规格或纯度 ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍

KGF角质细胞生长因子(Keratinocyte growth factor,KGF)是Rubin等(1989)从胚胎肺成纤维细胞培养上清中发现的一种细胞因子,为FGF 家族成员,即FGF-7。KGF由间质细胞分泌,受体分布于上皮细胞,是一种有效的上皮细胞特异性生长因子,其有丝分裂活性主要表现在角质形成细胞,可特异性地促进上皮细胞增殖、迁移及分化,与器官发育、创面修复、肿瘤发生及免疫重建等多方面关系密切。

添加培养MCF-7细胞增殖法测定,ED50值低于1.0 ng/ml,即对应活性单位大于等于1 x 10*6 units/mg.

 pH值: 6.5-7.5

OsrhKGF是运用基因重组技术,由水稻胚乳细胞表达并通过蛋白提纯工艺而成。



Keratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelial cells. Its mitotic activity is mainly manifested in keratinocytes, which can specifically promote the proliferation, migration and differentiation of epithelial cells. It is closely related to organ development, wound repair, tumor genesis and immune reconstruction.
Activity definition: The ED50 value is less than 1.0 ng/ml, that is, the corresponding activity unit is greater than or equal to 1 x 10*6 units/mg, as determined by the proliferation method of cultured MCF-7 cells.

生物活性 Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <10 ng/mL.
内毒素水平 <0.1 EU/μg
表达系统 水稻(Oryza sativa)
种属 人(Human)
氨基酸 32-194 aa
序列 MCNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLP MAIT
蛋白标签 No tag
无载体 No
无动物源 Yes
Accession # P21781.1
来源 重组表达
预测分子量 19.0 kD

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 pH 6.5~7.5 (L-组氨酸,海藻糖)
防腐剂 No
复溶 建议用无菌水重溶OsrhKGF冻干粉至100-200 ug/ml,可用其他溶剂进行进一步稀释。溶解后的OsrhKGF 4℃可保存2-7天,尽快用完。若短期不用,请置于-20℃保存。 开启后,请尽快使用,避免污染。
储存温度 -20°C储存
运输条件 超低温冰袋运输
稳定性与储存 Shipped at 4°C. Store at +4°C short term (2-7 days). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Resolution: It is recommended to redissolve lyophilized powder with sterile water to 100-200 ug/ml, and further dilute it with other so
CAS编号和信息 148348-15-6
分子类型 蛋白质

图片

Recombinant Human KGF Protein (rp155960)-Protein Bioactivity
Measured in a cell proliferation assay using MCF7 breast cancer cell line. The ED₅₀ for this effect is typically <10ng/mL.

Recombinant Human KGF Protein (rp155960)-SDS-PAGE
2μg/lane of Recombinant Human KGF was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16kDa. Another band is the HSA protein.

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批号(Lot Number) 证书类型 货号
ZJ23F0300075 分析证书 rp155960
ZJ23F0300079 分析证书 rp155960
ZJ23F0300074 分析证书 rp155960
ZJ25F0319988 分析证书 rp155960
ZJ25F0319987 分析证书 rp155960
ZJ25F0319986 分析证书 rp155960

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Mammalian Cell Cryopreservation Protocol
Mammalian Cell Cryopreservation Protocol

溶液计算器