Recombinant Rat IL-6 Protein

COA

级别和纯度:

≥96%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: P20607
蛋白标签: No tag
生物活性: 1. Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/mL, corresponding to a specific activity of >1.0 ×108 IU/mg. 2. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is less than 10 ng/mL.
内毒素水平: <0.1 EU/μg

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
rp155124-10μg	10μg	现货
rp155124-50μg	50μg	现货
rp155124-100μg	100μg	现货
rp155124-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL6

Accession#:P20607 (1) Gene ID:24498 (1) IL6 (25)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本描述

产品名称	Recombinant Rat IL-6 Protein
别名	重组大鼠 IL-6 蛋白
英文别名	B-cell differentiation factor \| B-cell stimulatory factor 2 \| BSF2 \| BSF-2 \| CDF \| CTL differentiation factor \| hybridoma growth factor \| MGI-2A \| Interleukin BSF 2 \| Cytotoxic T cell differentiation factor \| Hepatocyte stimulating factor \| Hepatocyte sti
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC)
产品介绍	Interleukin-6 (IL-6) is encoded by the IL-6 gene and acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant Rat IL-6 is a 21.7kDa protein containing 187 amino acid residues. Purity >96% SDS-PAGE Function Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
生物活性	1. Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/mL, corresponding to a specific activity of >1.0 ×108 IU/mg. 2. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is less than 10 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	E. coli
种属	大鼠(Rat)
氨基酸	25-211 aa
序列	FPTSQVRRGDFTEDTTHNRPVYTTSQVGGLITYVLREILEMRKELCNGN SDCMNSDDALSENNLKLPEIQRNDGCFQTGYNQEICLLKICSGLLEFRFY LEFVKNNLQDNKKDKARVIQSNTETLVHIFKQEIKDSYKIVLPTPTSNAL LMEKLESQKEWLRTKTIQLILKALEEFLKVTMRSTRQT
纯度	＞96% (SDS-PAGE, HPLC)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	P20607
来源	重组表达
预测分子量	21.7 kDa
SDS-PAGE	20.9 kDa, under reducing conditions; 19.1 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4
复溶	This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-80℃储存
运输条件	超低温冰袋运输
稳定性与储存	在 4°C 下运输。储存温度为 -80°C。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Rat IL-6 Protein (rp155124)

Recombinant Rat IL-6 Protein (rp155124) - Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less than 0.01 ng/mL, corresponding to a specific activity of >1.0 ×10⁸ IU/mg.

Protein Bioactivity: Recombinant Rat IL-6 Protein (rp155124)

Recombinant Rat IL-6 Protein (rp155124) - Protein Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is less than 10 ng/mL.

SDS-PAGE: Recombinant Rat IL-6 Protein (rp155124)

Recombinant Rat IL-6 Protein (rp155124)- SDS-PAGE
2.5 μg/lane of Recombinant Rat IL-6 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 20.9 kDa under reducing conditions and 19.1 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到5个结果

批号(Lot Number)	证书类型	货号
ZJ25F0724214	分析证书	rp155124
ZJ25F0724213	分析证书	rp155124
ZJ25F0724212	分析证书	rp155124
ZJ23F0901077	分析证书	rp155124
ZJ23F0901076	分析证书	rp155124

技术文档和文章

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度