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Recombinant mouse IL-7 protein

  • 表达系统: E. coli
  • Accession #: Q544C8
  • 蛋白标签: No tag
  • 生物活性: Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is less than 1ng/mL。
  • 内毒素水平: <0.01 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp154245-2μg
2μg 现货 Stock Image
rp154245-10μg
10μg 现货 Stock Image
rp154245-100μg
100μg 现货 Stock Image
rp154245-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant mouse IL-7 protein
别名 重组小鼠白细胞介素7蛋白 | 重组小鼠 IL-7 蛋白
英文别名 IL7 | IL-7 | interleukin-7 | interleukin 7 | Lymphopoietin-1 | PBGF | hlb368 | A630026I06Rik
规格或纯度 ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE)
产品介绍


Purity

>97% SDS-PAGE.


Interleukin-7 (IL-7) is encoded by the IL7 gene in mouse and secreted by stromal cells in the red marrow and thymus. The protein signals through the IL-7 receptor, which is a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and it can stimulate proliferation of B cells, T cells and NK cells. Mouse IL-7 has approximately 65 % and 88 % amino acid sequence identity with human and rat IL-7 and both proteins exhibit cross-species activity. Recombinant Mouse IL-7 is a 14.9kDa globular protein containing 129 amino acid residues.


Function

Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation.

生物活性 Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is less than 1ng/mL。
内毒素水平 <0.01 EU/μg
表达系统 E. coli
种属 小鼠(Mouse)
氨基酸 26-154 aa
序列 ECHIKDKEGKAYESVLMISIDELDKMTGTDSNCPNNEPNFFRKHVCDDTKEAAFLNRAARKLKQFLKMNISEEFNVHLLTVSQGTQTLVNCTSKEEKNVKEQKKNDACFLKRLLREIKTCWNKILKGSI
纯度 >97%(SDS-PAGE)
蛋白标签 No tag
N-末端序列 Glu-Cys-His-Ile-Lys-Asp-Lys-Glu
蛋白长度 Full length protein
无载体 Yes
无动物源 Yes
Accession # Q544C8
来源 重组表达
预测分子量 14.9 kDa
SDS-PAGE 12.9 kDa, under reducing conditions; 12.1 kDa, under non-reducing conditions.

储存与运输

应用 Active, SDS-PAGE
物理形态 冻干(Lyophilized)
储存缓冲液 20mM PB,150mM NaCl, pH 7.4 containing 3% trehalose
复溶 This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile aqueous buffer to an appropriate concentration.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 白色冻干粉末。如需长期保存,本品应按供货状态≤-20℃保存 36 个月,-20 至 -70℃。1 个月,复溶后无菌条件下 2 至 8℃;3 个月,复溶后无菌条件下 -20 至 -70℃。
分子类型 蛋白质

图片

Recombinant Mouse IL-7 Protein (rp154245)-Protein Bioactivity
Measured in a cell proliferation assay using PHA-activated human peripheral blood mononuclear cell (PBMC). The ED50 for this effect is typically <1ng/mL.

Recombinant Mouse IL-7 Protein (rp154245)-SDS-PAGE
3μg/lane of Recombinant Mouse IL-7 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a single band at 12.9 kDa under reducing conditions and 12.1 kDa under non-reducing conditions.

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批号(Lot Number) 证书类型 货号
ZJ25F0623043 分析证书 rp154245
ZJ25F0623042 分析证书 rp154245
ZJ25F0623041 分析证书 rp154245
ZJ23F0600297 分析证书 rp154245
ZJ23F0600296 分析证书 rp154245
ZJ23F0600295 分析证书 rp154245

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