Recombinant Human RANTES Protein

COA

级别和纯度:

≥98%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: P13501
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp150860-10μg	10μg	现货
rp150860-20μg	20μg	期货
rp150860-50μg	50μg	现货
rp150860-100μg	100μg	现货
rp150860-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CCL5

Accession#:P13501 (2) CCL5 (2) Gene ID:6352 (2) RANTES (2)

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基本描述

产品名称	Recombinant Human RANTES Protein
别名	重组人RANTES蛋白 \| 重组人类RANTES蛋白
英文别名	CCL5 \| chemokine (C-C motif) ligand 5 \| D17S136Enormally T-expressed, and presumably secreted \| EoCP \| Eosinophil chemotactic cytokine \| RANTES \| SISd \| SIS-delta \| small inducible cytokine A5 (RANTES) \| small inducible cytokine subfamily A (Cys-Cys), mem
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE&HPLC)
产品介绍	Purity >98% by SDS-PAGE and HPLC analyses. Function Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
生物活性	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	24-91 aa
序列	SPYSSDTTPCCFAYIARPLPRAHIKEYFYTSGKCSNPAVVFVTRKNRQVCANPEKKWVREYINSLEMS
纯度	＞98%(SDS-PAGE, HPLC)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
Accession #	P13501
来源	重组表达
预测分子量	7.8 kDa
SDS-PAGE	7.8 kDa

储存与运输

应用	Cell Culture
物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 100 mM NaCl.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	使用手动除霜冰柜，避免反复冻融循环。自收到之日起 12 个月，-20 至 -70 °C。复溶后 1 个月，无菌条件下 2 至 8 °C。3 个月，复溶后在无菌条件下 -20 至 -70 °C。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human RANTES Protein (rp150860)

Recombinant Human RANTES Protein (rp150860)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/mL.

SDS-PAGE: Recombinant Human RANTES Protein (rp150860)

Recombinant Human RANTES Protein (rp150860)-SDS-PAGE
Recombinant Human RANTES Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, Showing a band at 7.8 kDa under reducing conditions and 9.6 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600402	分析证书	rp150860
ZJ23F0600404	分析证书	rp150860
ZJ23F0600403	分析证书	rp150860

技术文档和文章

引用文献

1. Ignatov A, Robert J, Gregory-Evans C, Schaller HC. (2006) RANTES stimulates Ca2+ mobilization and inositol trisphosphate (IP3) formation in cells transfected with G protein-coupled receptor 75.. Br J Pharmacol, 149 (5): (490-7). [PMID:17001303]
2. Cocchi F, DeVico AL, Garzino-Demo A, Arya SK, Gallo RC, Lusso P. (1995) Identification of RANTES, MIP-1 alpha, and MIP-1 beta as the major HIV-suppressive factors produced by CD8+ T cells.. Science, 270 (5243): (1811-5). [PMID:8525373]
3. Regenass P, Abboud D, Daubeuf F, Lehalle C, Gizzi P, Riché S, Hachet-Haas M, Rohmer F, Gasparik V, Boeglin D et al.. (2018) Discovery of a Locally and Orally Active CXCL12 Neutraligand (LIT-927) with Anti-inflammatory Effect in a Murine Model of Allergic Airway Hypereosinophilia.. J Med Chem, 61 (17): (7671-7686). [PMID:30106292]
4. Wichapong K, Alard JE, Ortega-Gomez A, Weber C, Hackeng TM, Soehnlein O, Nicolaes GA.. (2016) Structure-Based Design of Peptidic Inhibitors of the Interaction between CC Chemokine Ligand 5 (CCL5) and Human Neutrophil Peptides 1 (HNP1).. J Med Chem, 59 (9): (4289-4301). [PMID:26871718] [10.1021/acs.jmedchem.5b01952]
5. Kameyoshi, Y Y, Dörschner, A A, Mallet, A I AI, Christophers, E E and Schröder, J M JM.. (1992) Cytokine RANTES released by thrombin-stimulated platelets is a potent attractant for human eosinophils.. The Journal of experimental medicine, (1): [PMID:1380064]
6. Schall, T J TJ and 6 more authors.. (1988) A human T cell-specific molecule is a member of a new gene family.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:2456327]
7. Schröder, J M JM, Kameyoshi, Y Y and Christophers, E E.. (1993) Platelets secrete an eosinophil-chemotactic cytokine which is a member of the C-C-chemokine family.. Advances in experimental medicine and biology, [PMID:7524281]
8. Skelton, N J NJ, Aspiras, F F, Ogez, J J and Schall, T J TJ.. (1995) Proton NMR assignments and solution conformation of RANTES, a chemokine of the C-C type.. Biochemistry, (25): [PMID:7537088]
9. Chung, C W CW, Cooke, R M RM, Proudfoot, A E AE and Wells, T N TN.. (1995) The three-dimensional solution structure of RANTES.. Biochemistry, (25): [PMID:7542919]
10. Proost, P P and 9 more authors.. (1998) Amino-terminal truncation of chemokines by CD26/dipeptidyl-peptidase IV. Conversion of RANTES into a potent inhibitor of monocyte chemotaxis and HIV-1-infection.. The Journal of biological chemistry, (27): [PMID:9516414]
11. Wilken, J J and 8 more authors.. (1999) Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES.. Chemistry & biology, [PMID:9889151]
12. Nomiyama, H H and 5 more authors.. (1999) Organization of the chemokine gene cluster on human chromosome 17q11.2 containing the genes for CC chemokine MPIF-1, HCC-2, HCC-1, LEC, and RANTES.. Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research, [PMID:10213461]
13. Lim, Jean K JK, Burns, Jennifer M JM, Lu, Wuyuan W and DeVico, Anthony L AL.. (2005) Multiple pathways of amino terminal processing produce two truncated variants of RANTES/CCL5.. Journal of leukocyte biology, [PMID:15923218]
14. Capoulade-Métay, Corinne C and 10 more authors.. (2006) A natural CCL5/RANTES variant antagonist for CCR1 and CCR3.. Immunogenetics, [PMID:16791620]
15. Lim, Jean K JK, Lu, Wuyuan W, Hartley, Oliver O and DeVico, Anthony L AL.. (2006) N-terminal proteolytic processing by cathepsin G converts RANTES/CCL5 and related analogs into a truncated 4-68 variant.. Journal of leukocyte biology, [PMID:16963625]
16. Caballero-Campo, Pedro P and 5 more authors.. (2014) A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis.. Journal of cellular physiology, [PMID:23765988]
17. Liu, Bo B and 7 more authors.. (2013) The novel chemokine receptor, G-protein-coupled receptor 75, is expressed by islets and is coupled to stimulation of insulin secretion and improved glucose homeostasis.. Diabetologia, [PMID:23979485]

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