Recombinant Human RANK Protein

COA

级别和纯度:

≥98%(SDS-PAGE)

表达系统: E. coli
Accession #: Q9Y6Q6
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp150851-10μg	10μg	现货
rp150851-50μg	50μg	现货
rp150851-100μg	100μg	现货
rp150851-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: TNFRSF11A

Accession#:Q9Y6Q6 (1) Gene ID:8792 (1) RANK (1) TNFRSF11A (1)

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基本描述

产品名称	Recombinant Human RANK Protein
别名	重组人 RANK蛋白 \| 核因子-κB 受体激活因子 \| 重组人类 RANK 蛋白
英文别名	CD 265 \| CD265 \| FEO \| LOH18CR1 \| Loss of heterozygosity 18 chromosomal region 1 \| mRANK \| ODFR \| OFE \| OPTB7 \| Osteoclast differentiation factor receptor \| OSTS \| Paget disease of bone 2 \| PDB 2 \| PDB2 \| RANK \| Receptor activator of NF KB \| Receptor acti
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE)
产品介绍	Purity >98% SDS-PAGE. Function Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis. Involved in the regulation of interactions between T-cells and dendritic cells.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/ml, corresponding to a specific activity of > 2.0 × 104 IU/mg in the presence of 15 ng/ml of recombinant sRANK Ligand.
内毒素水平	<0.1 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	29-202 aa
序列	QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK
纯度	＞98%(SDS-PAGE)
蛋白标签	No tag
蛋白长度	Protein fragment
无载体	Yes
无动物源	Yes
Accession #	Q9Y6Q6
来源	重组表达
预测分子量	19.1 kDa
SDS-PAGE	20.5 kDa, under reducing conditions; 18.2 kDa, under non-reducing conditions.

储存与运输

应用	Cell Culture,Active,SDS-PAGE
物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150mM
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 2-8 ℃ 下保存一个月。分装后在 -80 ℃ 保存 12 个月。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human RANK protein (rp150851)

Recombinant Human RANK protein (rp150851) - SDS-PAGE
Recombinant Human RANK protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 20.5 kDa under reducing conditions and 18.2 kDa under non-reducing conditions.

Protein Bioactivity: Recombinant Human RANK protein (rp150851)

Recombinant Human RANK protein (rp150851) - Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit RANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 10⁴ IU/mg in the presence of 15 ng/mL of recombinant RANK Ligand.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0700625	分析证书	rp150851
ZJ23F0700626	分析证书	rp150851
ZJ23F0700627	分析证书	rp150851

技术文档和文章

引用文献

1. Anderson, D M DM and 9 more authors.. (1997) A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic-cell function.. Nature, (13): [PMID:9367155]
2. Wong, B R BR and 5 more authors.. (1998) The TRAF family of signal transducers mediates NF-kappaB activation by the TRANCE receptor.. The Journal of biological chemistry, (23): [PMID:9774460]
3. Nakagawa, N N and 7 more authors.. (1998) RANK is the essential signaling receptor for osteoclast differentiation factor in osteoclastogenesis.. Biochemical and biophysical research communications, (18): [PMID:9878548]
4. Hughes, A E AE and 10 more authors.. (2000) Mutations in TNFRSF11A, affecting the signal peptide of RANK, cause familial expansile osteolysis.. Nature genetics, [PMID:10615125]
5. Wada, Teiji T and 6 more authors.. (2005) The molecular scaffold Gab2 is a crucial component of RANK signaling and osteoclastogenesis.. Nature medicine, [PMID:15750601]
6. Guerrini, Matteo M MM and 20 more authors.. (2008) Human osteoclast-poor osteopetrosis with hypogammaglobulinemia due to TNFRSF11A (RANK) mutations.. American journal of human genetics, [PMID:18606301]
7. Dephoure, Noah N and 6 more authors.. (2008) A quantitative atlas of mitotic phosphorylation.. Proceedings of the National Academy of Sciences of the United States of America, (5): [PMID:18669648]
8. Zhou, Houjiang H and 6 more authors.. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome.. Journal of proteome research, (4): [PMID:23186163]
9. Sirinian, Chaido C, Papanastasiou, Anastasios D AD, Zarkadis, Ioannis K IK and Kalofonos, Haralabos P HP.. (2013) Alternative splicing generates a truncated isoform of human TNFRSF11A (RANK) with an altered capacity to activate NF-κB.. Gene, (1): [PMID:23664977]

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