基本描述

产品名称	Recombinant Human RAGE Protein
别名	重组人晚期糖基化终末产物受体 \| 重组人类 RAGE 蛋白
英文别名	advanced glycosylation end product-specific receptor \| AGER \| RAGE isoform delta \| RAGE isoform sRAGE-delta \| RAGE \| Receptor for advanced glycosylation end products \| receptor for advanced glycosylation end-products \| SCARJ1 \| sRAGE \| Receptor for advanc
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥90%(SDS-PAGE)
产品介绍	Purity ＞90% SDS-PAGE. Function Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
生物活性	Recombinant human HMGB1 at 2 μg/mL (100 μL/well) can bind Recombinant human AGER with a linear range of 15-50 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	24-344 aa
序列	QNITARIGEPLVLKCKGAPKKPPQRLEWKLNTGRTEAWKVLSPQGGGPWDSVARVLPNGSLFLPAVGIQDEGIFRCQAMNRNGKETKSNYRVRVYQIPGKPEIVDSASELTAGVPNKVGTCVSEGSYPAGTLSWHLDGKPLVPNEKGVSVKEQTRRHPETGLFTLQSELMVTPARGGDPRPTFSCSFSPGLPRHRALRTAPIQPRVWEPVPLEEVQLVVEPEGGAVAPGGTVTLTCEVPAQPSPQIHWMKDGVPLPLPPSPVLILPEIGPQDQGTYSCVATHSSHGPQESRAVSISIIEPGEEGPTAGSVGGSGLGTLALAENLYFQGMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH
纯度	＞90%(SDS-PAGE)
蛋白标签	C-hFc & His
无动物源	Yes
Accession #	Q15109
来源	重组表达
预测分子量	63 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4, 5% Trehalose, 5% Mannitol
复溶	Reconstitute in water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human RAGE Protein (rp150830)

Recombinant Human RAGE Protein (rp150830) - Protein Bioactivity
Recombinant human HMGB1 at 2.0 μg/mL can bind Recombinant Human RAGE Protein (rp150830) with a linear range of 15.0 -50.0 ng/mL. The EC₅₀ for this effect is 70.0 ng/mL.

SDS-PAGE: Recombinant Human RAGE Protein (rp150830)

Recombinant Human RAGE Protein (rp150830) - SDS-PAGE
3 μg/lane of Recombinant Human RAGE was resolved with SDS-PAGE under reducing (R) conditions and visualized by Coomassie® Blue staining, showing the band at 77.1 & 38.1 kDa under reducing conditions.
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质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到6个结果

批号(Lot Number)	证书类型	货号
ZJ24F1215483	分析证书	rp150830
ZJ24F1215481	分析证书	rp150830
ZJ24F1215482	分析证书	rp150830
ZJ24F0505697	分析证书	rp150830
ZJ24F0505698	分析证书	rp150830
ZJ24F0505699	分析证书	rp150830

技术文档和文章

阿拉丁®靶点蛋白

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引用文献

1. Neeper M, Schmidt AM, Brett J, Yan SD, Wang F, Pan YC, Elliston K, Stern D, Shaw A. (1992) Cloning and expression of a cell surface receptor for advanced glycosylation end products of proteins.. J Biol Chem, 267 (21): (14998-5004). [PMID:1378843]
2. Saito S, Ihara M. (2014) New therapeutic approaches for Alzheimer's disease and cerebral amyloid angiopathy.. Front Aging Neurosci, 6 (290). [PMID:25368578]
3. Bongarzone S, Savickas V, Luzi F, Gee AD. (2017) Targeting the Receptor for Advanced Glycation Endproducts (RAGE): A Medicinal Chemistry Perspective.. J Med Chem, 60 (17): (7213-7232). [PMID:28482155]
4. Cherilyn M Sirois,Tengchuan Jin,Allison L Miller,Damien Bertheloot,Hirotaka Nakamura,Gabor L Horvath,Abubakar Mian,Jiansheng Jiang,Jacob Schrum,Lukas Bossaller,Karin Pelka,Natalio Garbi,Yambasu Brewah,Jane Tian,ChewShun Chang,Partha S Chowdhury,Gary P Sims,Roland Kolbeck,Anthony J Coyle,Alison A Humbles,T Sam Xiao,Eicke Latz. (2013-10-02) RAGE is a nucleic acid receptor that promotes inflammatory responses to DNA.. The Journal of experimental medicine, 210 ((11)): (2447-2463). [PMID:24081950]
5. Han YT, Kim K, Choi GI, An H, Son D, Kim H, Ha HJ, Son JH, Chung SJ, Park HJ, Lee J, Suh YG.. (2014) Pyrazole-5-carboxamides, novel inhibitors of receptor for advanced glycation end products (RAGE).. Eur J Med Chem, 79 (128-142). [PMID:24727489] [10.1016/j.ejmech.2014.03.072]
6. Choi K, Lim KS, Shin J, Kim SH, Suh YG, Hong HS, Kim H, Ha HJ, Kim YH, Lee J, Lee J.. (2015) 6-Phenoxy-2-phenylbenzoxazoles, novel inhibitors of receptor for advanced glycation end products (RAGE).. Bioorg Med Chem, 23 (15): (4919-4935). [PMID:26051601] [10.1016/j.bmc.2015.05.022]
7. Sugaya, K K and 7 more authors.. (1994) Three genes in the human MHC class III region near the junction with the class II: gene for receptor of advanced glycosylation end products, PBX2 homeobox gene and a notch homolog, human counterpart of mouse mammary tumor gene int-3.. Genomics, (15): [PMID:7835890]
8. Hudson, B I BI, Stickland, M H MH, Futers, T S TS and Grant, P J PJ.. (2001) Effects of novel polymorphisms in the RAGE gene on transcriptional regulation and their association with diabetic retinopathy.. Diabetes, [PMID:11375354]
9. Yonekura, Hideto H and 12 more authors.. (2003) Novel splice variants of the receptor for advanced glycation end-products expressed in human vascular endothelial cells and pericytes, and their putative roles in diabetes-induced vascular injury.. The Biochemical journal, (15): [PMID:12495433]
10. Xie, Tao T and 7 more authors.. (2003) Analysis of the gene-dense major histocompatibility complex class III region and its comparison to mouse.. Genome research, [PMID:14656967]
11. Hudson, Barry I BI and 7 more authors.. (2008) Identification, classification, and expression of RAGE gene splice variants.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:18089847]
12. Moroz, Olga V OV and 13 more authors.. (2009) Both Ca2+ and Zn2+ are essential for S100A12 protein oligomerization and function.. BMC biochemistry, (23): [PMID:19386136]
13. Fang, Fang F and 11 more authors.. (2010) RAGE-dependent signaling in microglia contributes to neuroinflammation, Abeta accumulation, and impaired learning/memory in a mouse model of Alzheimer's disease.. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, [PMID:19906677]
14. Park, Hajeung H, Adsit, Floyd G FG and Boyington, Jeffrey C JC.. (2010) The 1.5 Å crystal structure of human receptor for advanced glycation endproducts (RAGE) ectodomains reveals unique features determining ligand binding.. The Journal of biological chemistry, (24): [PMID:20943659]
15. Koch, Michael M and 6 more authors.. (2010) Structural basis for ligand recognition and activation of RAGE.. Structure (London, England : 1993), (13): [PMID:20947022]
16. Jin, Qing'e Q, Chen, Hongyan H, Luo, Aiping A, Ding, Fang F and Liu, Zhihua Z.. (2011) S100A14 stimulates cell proliferation and induces cell apoptosis at different concentrations via receptor for advanced glycation end products (RAGE).. PloS one, (29): [PMID:21559403]
17. Xue, Jing J and 9 more authors.. (2011) Advanced glycation end product recognition by the receptor for AGEs.. Structure (London, England : 1993), (11): [PMID:21565706]
18. Sakaguchi, Masakiyo M and 8 more authors.. (2011) TIRAP, an adaptor protein for TLR2/4, transduces a signal from RAGE phosphorylated upon ligand binding.. PloS one, [PMID:21829704]
19. Wei, Wen W and 9 more authors.. (2012) Disulfide bonds within the C2 domain of RAGE play key roles in its dimerization and biogenesis.. PloS one, [PMID:23284645]
20. Jules, Joel J, Maiguel, Dony D and Hudson, Barry I BI.. (2013) Alternative splicing of the RAGE cytoplasmic domain regulates cell signaling and function.. PloS one, [PMID:24260107]
21. Shang, Dan and 6 more authors.. (2016) High Mobility Group Box Protein 1 Boosts Endothelial Albumin Transcytosis through the RAGE/Src/Caveolin-1 Pathway.. Scientific reports, (30): [PMID:27572515]

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所需的复溶浓度

货号 (SKU)	包装规格	是否现货
rp150830-10μg	10μg	现货
rp150830-50μg	50μg	现货
rp150830-100μg	100μg	现货
rp150830-1mg	1mg	期货

Recombinant Human RAGE Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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引用文献

溶液计算器

摩尔浓度计算器

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