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Recombinant Human Melanoma Inhibitory Activity Protein

Recombinant Human Melanoma Inhibitory Activity Protein

级别和纯度:

≥97%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: Q16674
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of > 200 IU/mg.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp148684-10μg	10μg	现货
rp148684-20μg	20μg	期货
rp148684-50μg	50μg	现货
rp148684-100μg	100μg	现货
rp148684-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: MIA

Accession#:Q16674 (1) Gene ID:8190 (1) Melanoma Inhibitory Activity (1) MIA (1)

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基本描述

产品名称	Recombinant Human Melanoma Inhibitory Activity Protein
别名	重组人黑色素瘤抑制活性蛋白 \| 重组人黑色素瘤抑制活性蛋白
英文别名	CD-RAP \| Melanoma inhibitory activity protein \| melanoma inhibitory activity \| melanoma-derived growth regulatory protein \| MIA \| Melanoma inhibitory activity protein
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
产品介绍	purity >97% by SDS-PAGE and HPLC analysesFunction Function Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. Post-translational May possess two intramolecular disulfide bonds.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/ml, corresponding to a specific activity of > 200 IU/mg.
内毒素水平	<0.1 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	25-131aa
序列	GPMPKLADRKLCADQECSHPISMAVALQDYMAPDCRFLTIHRGQVVYVFSKLKGRGRLFW GGSVQGDYYGDLAARLGYFPSSIVREDQTLKPGKVDVKTDKWDFYCQ
纯度	＞97%( SDS-PAGE and HPLC)
蛋白标签	No tag
蛋白长度	Protein fragment
无载体	Yes
无动物源	Yes
Accession #	Q16674
来源	重组表达
预测分子量	12.1 kDa
SDS-PAGE	12.1 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.
复溶	Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	收货后建议分装，储存于 -20°C，避免冷冻/解冻循环。本产品为活性蛋白质，可能会引起体内生物反应，请谨慎处理。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human Melanoma Inhibitory Activity Protein (rp148684)

Recombinant Human Melanoma Inhibitory Activity Protein (rp148684)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human A375 cell line is less than 5 μg/mL, corresponding to a specific activity of > 200 IU/mg.

SDS-PAGE: Recombinant Human Melanoma Inhibitory Activity Protein (rp148684)

Recombinant Human Melanoma Inhibitory Activity Protein (rp148684)-SDS-PAGE
Recombinant Human Melanoma Inhibitory Activity Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining. Showing a band at 12.1 kDa under reducing conditions and 10.3 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600335	分析证书	rp148684
ZJ23F0600336	分析证书	rp148684
ZJ23F0600337	分析证书	rp148684

技术文档和文章

引用文献

1. Blesch, A A and 9 more authors.. (1994) Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA.. Cancer research, (1): [PMID:7923218]
2. Bosserhoff, A K AK, Hein, R R, Bogdahn, U U and Buettner, R R.. (1996) Structure and promoter analysis of the gene encoding the human melanoma-inhibiting protein MIA.. The Journal of biological chemistry, (5): [PMID:8550608]
3. Lougheed, J C JC, Holton, J M JM, Alber, T T, Bazan, J F JF and Handel, T M TM.. (2001) Structure of melanoma inhibitory activity protein, a member of a recently identified family of secreted proteins.. Proceedings of the National Academy of Sciences of the United States of America, (8): [PMID:11331761]
4. Hau, Peter P and 7 more authors.. (2002) Cloning and characterization of the expression pattern of a novel splice product MIA (splice) of malignant melanoma-derived growth-inhibiting activity (MIA/CD-RAP) [corrected].. The Journal of investigative dermatology, [PMID:12230496]
5. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
6. Voss, Matthias M, Lettau, Marcus M and Janssen, Ottmar O.. (2009) Identification of SH3 domain interaction partners of human FasL (CD178) by phage display screening.. BMC immunology, (6): [PMID:19807924]
7. Rahimi, Nader N, Rezazadeh, Kobra K, Mahoney, John E JE, Hartsough, Edward E and Meyer, Rosana D RD.. (2012) Identification of IGPR-1 as a novel adhesion molecule involved in angiogenesis.. Molecular biology of the cell, [PMID:22419821]

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