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Recombinant Human LIF Protein

COA COA
  • 表达系统: HEK293
  • Accession #: P15018
  • 蛋白标签: C-His
  • 生物活性: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng/mL.
  • 内毒素水平: <0.1 EU/μg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp148270-10μg
 10μg 现货 Stock Image
rp148270-50μg
 50μg 现货 Stock Image
rp148270-100μg
 100μg 现货 Stock Image
rp148270-1mg
 1mg 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
查看相关系列
点击查看蛋白信息: LIF
Accession#:P15018 (5) Gene ID:3976 (5) LIF (6)
main product photo

基本描述

产品名称 Recombinant Human LIF Protein
别名 白血病抑制因子(LIF)活性蛋白 | 重组人LIF蛋白 | 重组人类 LIF 蛋白
英文别名 CDF | D Factor | DIA | differentiation inhibitory activity | differentiation stimulating factor | Differentiation-stimulating factor | Emfilermin | HILDA | HILDAcholinergic differentiation factor | leukemia inhibitory factor (cholinergic differentiation f
规格或纯度 ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍


Purity
≥95% (SDS-PAGE)
Endotoxin level
＜0.1 EU/μg
Function
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
生物活性 Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.1-0.3 ng/mL.
内毒素水平 <0.1 EU/μg
表达系统 HEK293
种属 人(Human)
氨基酸 23-202 aa
序列 SPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNVTDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNVLCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAFHHHHHH
纯度 ≥95% (SDS-PAGE)
蛋白标签 C-His
无动物源 Yes
Accession # P15018
来源 重组表达
预测分子量 20 kDa
SDS-PAGE 33-45 kDa, under reducing conditions

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶 Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型 蛋白质

图片

Recombinant Human LIF Protein (rp148270) - SDS-PAGE
3 μg/lane of Recombinant Human LIF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 35 - 45 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到6个结果

批号(Lot Number) 证书类型 货号
ZJ24F1215601 分析证书 rp148270
ZJ24F1215602 分析证书 rp148270
ZJ24F1215603 分析证书 rp148270
ZJ24F0506245 分析证书 rp148270
ZJ24F0506246 分析证书 rp148270
ZJ24F0506247 分析证书 rp148270

可替换产品

  1. Recombinant Human LIF Protein
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  2. Recombinant Human LIF Protein
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  3. Recombinant Human LIF Protein
    Recombinant Human LIF Protein

    ¥719.90

引用文献

1. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039]
2. Stahl, J J and 5 more authors..  (1990)  Structural organization of the genes for murine and human leukemia inhibitory factor. Evolutionary conservation of coding and non-coding regions..  The Journal of biological chemistry,  (25): [PMID:1692837]
3. Lowe, D G DG and 9 more authors..  (1989)  Genomic cloning and heterologous expression of human differentiation-stimulating factor..  DNA (Mary Ann Liebert, Inc.),  [PMID:2475312]
4. Mori, M M, Yamaguchi, K K and Abe, K K..  (1989)  Purification of a lipoprotein lipase-inhibiting protein produced by a melanoma cell line associated with cancer cachexia..  Biochemical and biophysical research communications,  (15): [PMID:2730639]
5. Gough, N M NM and 6 more authors..  (1988)  Molecular cloning and expression of the human homologue of the murine gene encoding myeloid leukemia-inhibitory factor..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:3128791]
6. Moreau, J F JF and 5 more authors..  (1988)  Leukaemia inhibitory factor is identical to the myeloid growth factor human interleukin for DA cells..  Nature,  (15): [PMID:3143918]
7. Schmelzer, C H CH and 5 more authors..  (1993)  Glycosylation pattern and disulfide assignments of recombinant human differentiation-stimulating factor..  Archives of biochemistry and biophysics,  [PMID:8489250]
8. Dunham, I I and 24 more authors..  (1999)  The DNA sequence of human chromosome 22..  Nature,  (2): [PMID:10591208]
9. Boulanger, Martin J MJ, Bankovich, Alexander J AJ, Kortemme, Tanja T, Baker, David D and Garcia, K Christopher KC..  (2003)  Convergent mechanisms for recognition of divergent cytokines by the shared signaling receptor gp130..  Molecular cell,  [PMID:14527405]
10. Brandenberger, Ralph R and 13 more authors..  (2004)  Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation..  Nature biotechnology,  [PMID:15146197]
11. Huyton, Trevor T and 6 more authors..  (2007)  An unusual cytokine:Ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (LIF) in complex with the LIF receptor..  Proceedings of the National Academy of Sciences of the United States of America,  (31): [PMID:17652170]

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