货号 (SKU)

包装规格

是否现货

价格

数量

rp147476-10μg

10μg

现货

rp147476-50μg

50μg

现货

rp147476-100μg

100μg

现货

rp147476-1mg

1mg

期货

基本描述

产品名称	Recombinant Human IL-2 Protein
别名	重组人白细胞介素-2蛋白 \| 重组人 IL-2 蛋白
英文别名	IL-2 \| T-cell growth factor \| TCGF
规格或纯度	ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	白介素2即白细胞介素-2（interleukin-2，IL-2），又名T细胞生长因子(T cell growth factor，TCRF)。主要由活化的CD4+T细胞和CD8+T细胞产生的具有广泛生物活性的细胞因子。是所有T细胞亚群的生长因子，能使T细胞在试管内长期存活，并可促进活化B细胞增殖，故为调控免疫应答的重要因子，也参与抗体反应、造血和肿瘤监视。本产品由真核细胞表达的重组蛋白，是将蛋白基因接入稳定表达载体，转化CHO-K1细胞，经克隆筛选，质量验证，挑选的稳定表达株，由化学成分确定的培养基（不含有任何动物源成分）发酵制备，工艺稳定，保证了产品质量及批次间的一致性。本产品为无菌冻干粉剂，由含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干。 Purity ≥95% (SDS-PAGE, SEC-HPLC) Additional sequence information 133 amino acids Function Interleukin-2, or interleukin-2, is also known as T cell growth factor (TCRF). A wide range of bioactive cytokines produced primarily by activated CD4+T cells and CD8+T cells. It is a growth factor of all T cell subsets, which can make T cells survive in vitro for a long time and promote the proliferation of activated B cells. Therefore, it is an important factor in the regulation of immune response, and also involved in antibody response, hematopoietic and tumor surveillance. Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. This product is a recombinant protein expressed by eukaryotic cells. The protein gene was inserted into stable expression vector and transformed into CHO-K1 cells. After cloning, screening and quality verification, the selected stable expression strain was prepared by fermentation of the medium determined by chemical composition (without any animal source components). This product is a sterile lyophilized powder, made from a protein solution containing 10mM phosphate with a pH of 7.2 filtered by 0.2μm.
生物活性	1、Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is typically less than 2.5 ng/mL. 2、Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	CHO-K1
种属	人(Human)
氨基酸	21-153 aa
序列	APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT
纯度	≥95% (SDS-PAGE, SEC-HPLC)
蛋白标签	His
蛋白长度	Full length protein
无载体	Yes
Accession #	P60568
来源	重组表达
预测分子量	15.4 kDa
SDS-PAGE	13.0 kDa, under reducing conditions; 13.0 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	含有 10mM 磷酸盐pH为 7.2的蛋白溶液经0.2μm过滤后分装冻干
复溶	建议将冻干 rhIL-2溶解在注射用水或灭菌的超纯水中，浓度不低于100μg/ml，以待进一步稀释至工作浓度。尽可能避免反复冻融。复溶后的细胞因子：4℃可稳定储存7-10天；短期保存请分装后存放于-20℃。
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	发货时温度为 4°C。储存温度为 -20°C。冻干，无添加剂。本品为活性蛋白质，可能会引起体内生物反应，请谨慎处理。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476)-Protein Bioactivity
Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED₅₀ for this effect is typically <2.5ng/mL.

SDS-PAGE: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476) -SDS-PAGE
3μg/lane of Recombinant Human IL-2 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 13.0 kDa.

Protein Bioactivity: Recombinant Human IL-2 Protein (rp147476)

Recombinant Human IL-2 Protein (rp147476) - Protein Bioactivity
Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically less than 30.0 ng/mL.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到7个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600485	分析证书	rp147476
ZJ23F0600486	分析证书	rp147476
ZJ24F1113698	分析证书	rp147476
ZJ24F1113697	分析证书	rp147476
ZJ24F1113623	分析证书	rp147476
ZJ24F1113622	分析证书	rp147476
ZJ23F0600484	分析证书	rp147476

技术文档和文章

阿拉丁®靶点蛋白

Categories: 技术文章

探索主题:

药物开发信号通路细胞因子靶点蛋白药物靶点受体免疫检查点细胞表面标记物

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引用文献

1. Quéméner A, Maillasson M, Arzel L, Sicard B, Vomiandry R, Mortier E, Dubreuil D, Jacques Y, Lebreton J, Mathé-Allainmat M. (2017) Discovery of a Small-Molecule Inhibitor of Interleukin 15: Pharmacophore-Based Virtual Screening and Hit Optimization.. J Med Chem, 60 (14): (6249-6272). [PMID:28657314]
2. Chen L, Du J, Dai Q, Zhang H, Pang W, Hu J.. (2014) Prediction of anti-tumor chemical probes of a traditional Chinese medicine formula by HPLC fingerprinting combined with molecular docking.. Eur J Med Chem, 83 (294-306). [PMID:24974349] [10.1016/j.ejmech.2014.06.037]
3. Żyżyńska-Granica B, Trzaskowski B, Niewieczerzał S, Filipek S, Zegrocka-Stendel O, Dutkiewicz M, Krzeczyński P, Kowalewska M, Koziak K.. (2017) Pharmacophore guided discovery of small-molecule interleukin 15 inhibitors.. Eur J Med Chem, 136 (543-547). [PMID:28535470] [10.1016/j.ejmech.2017.05.034]
4. Laâbi, Y Y and 6 more authors.. (1992) A new gene, BCM, on chromosome 16 is fused to the interleukin 2 gene by a t(4;16)(q26;p13) translocation in a malignant T cell lymphoma.. The EMBO journal, [PMID:1396583]
5. Mott, H R HR and 5 more authors.. (1992) Secondary structure of human interleukin 2 from 3D heteronuclear NMR experiments.. Biochemistry, (25): [PMID:1510960]
6. and Bazan, J F JF.. (1992) Unraveling the structure of IL-2.. Science (New York, N.Y.), (17): [PMID:1631562]
7. Conradt, H S HS and 5 more authors.. (1989) Expression of human interleukin-2 in recombinant baby hamster kidney, Ltk-, and Chinese hamster ovary cells. Structure of O-linked carbohydrate chains and their location within the polypeptide.. The Journal of biological chemistry, (15): [PMID:2793860]
8. Weir, M P MP, Chaplin, M A MA, Wallace, D M DM, Dykes, C W CW and Hobden, A N AN.. (1988) Structure-activity relationships of recombinant human interleukin 2.. Biochemistry, (6): [PMID:3264184]
9. Siebenlist, U U and 7 more authors.. (1986) Promoter region of interleukin-2 gene undergoes chromatin structure changes and confers inducibility on chloramphenicol acetyltransferase gene during activation of T cells.. Molecular and cellular biology, [PMID:3491296]
10. Brandhuber, B J BJ, Boone, T T, Kenney, W C WC and McKay, D B DB.. (1987) Three-dimensional structure of interleukin-2.. Science (New York, N.Y.), (18): [PMID:3500515]
11. Devos, R R and 7 more authors.. (1983) Molecular cloning of human interleukin 2 cDNA and its expression in E. coli.. Nucleic acids research, (11): [PMID:6306584]
12. Maeda, S S and 9 more authors.. (1983) Cloning of interleukin 2 mRNAs from human tonsils.. Biochemical and biophysical research communications, (30): [PMID:6312994]
13. Fujita, T T, Takaoka, C C, Matsui, H H and Taniguchi, T T.. (1983) Structure of the human interleukin 2 gene.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6324170]
14. Holbrook, N J NJ, Lieber, M M and Crabtree, G R GR.. (1984) DNA sequence of the 5' flanking region of the human interleukin 2 gene: homologies with adult T-cell leukemia virus.. Nucleic acids research, (25): [PMID:6330695]
15. Robb, R J RJ, Kutny, R M RM, Panico, M M, Morris, H R HR and Chowdhry, V V.. (1984) Amino acid sequence and post-translational modification of human interleukin 2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6333684]
16. Taniguchi, T T and 6 more authors.. (1983) Structure and expression of a cloned cDNA for human interleukin-2.. Nature, [PMID:6403867]
17. Holbrook, N J NJ and 5 more authors.. (1984) T-cell growth factor: complete nucleotide sequence and organization of the gene in normal and malignant cells.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6608729]
18. Bamborough, P P, Hedgecock, C J CJ and Richards, W G WG.. (1994) The interleukin-2 and interleukin-4 receptors studied by molecular modelling.. Structure (London, England : 1993), (15): [PMID:7529123]
19. Eizenberg, O O, Faber-Elman, A A, Lotan, M M and Schwartz, M M.. (1995) Interleukin-2 transcripts in human and rodent brains: possible expression by astrocytes.. Journal of neurochemistry, [PMID:7722480]
20. Miyazaki, T T and 9 more authors.. (1994) Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits.. Science (New York, N.Y.), (11): [PMID:7973659]
21. Chernicky, C L CL, Tan, H H, Burfeind, P P, Ilan, J J and Ilan, J J.. (1996) Sequence of interleukin-2 isolated from human placental poly A+ RNA: possible role in maintenance of fetal allograft.. Molecular reproduction and development, [PMID:8824916]
22. Wang, Xinquan X, Rickert, Mathias M and Garcia, K Christopher KC.. (2005) Structure of the quaternary complex of interleukin-2 with its alpha, beta, and gammac receptors.. Science (New York, N.Y.), (18): [PMID:16293754]
23. Stauber, Deborah J DJ, Debler, Erik W EW, Horton, Patricia A PA, Smith, Kendall A KA and Wilson, Ian A IA.. (2006) Crystal structure of the IL-2 signaling complex: paradigm for a heterotrimeric cytokine receptor.. Proceedings of the National Academy of Sciences of the United States of America, (21): [PMID:16477002]
24. Fiebich, Bernd L BL and 6 more authors.. (2012) Pseudoephedrine inhibits T-cell activation by targeting NF-κB, NFAT and AP-1 signaling pathways.. Immunopharmacology and immunotoxicology, [PMID:21631396]

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体积（添加到小瓶中）

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所需的复溶浓度

Recombinant Human IL-2 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

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