Recombinant Human IL-17RA/IL-17R Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q96F46
蛋白标签: C-hFc & His
生物活性: Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.03‑0.1 µg/mL in the presence of 10 ng/mL recombinant human IL‑17.
内毒素水平: <0.01 EU/μg

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货号 (SKU)	包装规格	是否现货
rp147441-10μg	10μg	现货
rp147441-50μg	50μg	现货
rp147441-100μg	100μg	现货
rp147441-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL17RA

Accession#:Q96F46 (3) Gene ID:23765 (3) IL17RA (3)

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基本描述

产品名称	Recombinant Human IL-17RA/IL-17R Protein
别名	重组人白细胞介素17受体蛋白 \| 重组人 IL-17RA/IL-17R 蛋白
英文别名	CD217 antigen \| CD217 \| Cdw217 \| CDw217interleukin 17 receptor \| hIL-17R \| IL-17 R \| IL-17 RA \| IL-17 receptor A \| IL17RA \| IL-17RA \| IL-17RAMGC10262 \| IL17Rinterleukin-17 receptor A \| interleukin 17 receptor A \| CANDF5 \| MGC10262 \| IMD51 \| CDw217 \| IL-17
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity ＞95% SDS-PAGE Function Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling.
生物活性	Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.03‑0.1 µg/mL in the presence of 10 ng/mL recombinant human IL‑17.
内毒素水平	<0.01 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	33-320 aa
序列	LRLLDHRALVCSQPGLNCTVKNSTCLDDSWIHPRNLTPSSPKDLQIQLHFAHTQQGDLFPVAHIEWTLQTDASILYLEGAELSVLQLNTNERLCVRFEFLSKLRHHHRRWRFTFSHFVVDPDQEYEVTVHHLPKPIPDGDPNHQSKNFLVPDCEHARMKVTTPCMSSGSLWDPNITVETLEAHQLRVSFTLWNESTHYQILLTSFPHMENHSCFEHMHHIPAPRPEEFHQRSNVTLTLRNLKGCCRHQVQIQPFFSSCLNDCLRHSATVSCPEMPDTPEPIPDYMPLWENLYFQGMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH
纯度	＞95%(SDS-PAGE)
蛋白标签	C-hFc & His
无载体	Yes
无动物源	Yes
Accession #	Q96F46
来源	重组表达
预测分子量	60 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	PBS(PH7.4), containing 5% trehalose, 5% mannitol
复溶	Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
储存温度	-20°C储存
运输条件	超低温冰袋运输
稳定性与储存	自收到之日起 12 个月，-20 至 -70 ℃。 1 个月，复溶后无菌条件下 2 至 8 ℃。 3 个月，复溶后无菌条件下 -20 至 -70 ℃。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-17RA/IL-17R Protein (rp147441)

Recombinant Human IL-17RA/IL-17R Protein (rp147441) - Protein Bioactivity
Measured by its ability to inhibit IL-17-induced IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.08 µg/mL in the presence of 10 ng/mL recombinant human IL-17.

Binding ELISA: Recombinant Human IL-17RA/IL-17R Protein (rp147441)

Recombinant Human IL-17RA/IL-17R Protein (rp147441) - Binding ELISA
IL-17RA/IL-17R Protein (rp147441) was serially diluted 3-fold and coated on a Clear Polystyrene Microplate (W291037) can bind Brodalumab (anti-IL17RA) (Ab169276). The EC₅₀ for this effect is 5.5 ng/mL.

SDS-PAGE: Recombinant Human IL-17RA/IL-17R Protein (rp147441)

Recombinant Human IL-17RA/IL-17R Protein (rp147441) - SDS-PAGE
2 μg/lane of Recombinant Human IL-17RA/IL-17R was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 91.3 kDa under reducing conditions and 208.1 kDa under non-reducing conditions.

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找到6个结果

批号(Lot Number)	证书类型	货号
ZJ23F1101654	分析证书	rp147441
ZJ23F1101652	分析证书	rp147441
ZJ23F1101653	分析证书	rp147441
ZJ23F1101809	分析证书	rp147441
ZJ23F1101807	分析证书	rp147441
ZJ23F1101808	分析证书	rp147441

技术文档和文章

引用文献

1. Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M et al.. (2008) The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.. J Immunol, 181 (4): (2799-805). [PMID:18684971]
2. Ramirez-Carrozzi V, Sambandam A, Luis E, Lin Z, Jeet S, Lesch J, Hackney J, Kim J, Zhou M, Lai J et al.. (2011) IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.. Nat Immunol, 12 (12): (1159-66). [PMID:21993848]
3. Yao, Z Z and 8 more authors.. (1997) Molecular characterization of the human interleukin (IL)-17 receptor.. Cytokine, [PMID:9367539]
4. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
5. Collins, John E JE and 12 more authors.. (2004) A genome annotation-driven approach to cloning the human ORFeome.. Genome biology, [PMID:15461802]
6. Toy, Dean D and 7 more authors.. (2006) Cutting edge: interleukin 17 signals through a heteromeric receptor complex.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:16785495]
7. Kuestner, Rolf E RE and 27 more authors.. (2007) Identification of the IL-17 receptor related molecule IL-17RC as the receptor for IL-17F.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:17911633]
8. Ely, Lauren K LK, Fischer, Suzanne S and Garcia, K Christopher KC.. (2009) Structural basis of receptor sharing by interleukin 17 cytokines.. Nature immunology, [PMID:19838198]
9. Puel, Anne A and 23 more authors.. (2011) Chronic mucocutaneous candidiasis in humans with inborn errors of interleukin-17 immunity.. Science (New York, N.Y.), (1): [PMID:21350122]
10. Sohda, Miwa M and 5 more authors.. (2013) Identification of a soluble isoform of human IL-17RA generated by alternative splicing.. Cytokine, [PMID:24084331]
11. Boisson, Bertrand B and 12 more authors.. (2013) An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis.. Immunity, (17): [PMID:24120361]
12. Goepfert, Arnaud A, Lehmann, Sylvie S, Wirth, Emmanuelle E and Rondeau, Jean-Michel JM.. (2017) The human IL-17A/F heterodimer: a two-faced cytokine with unique receptor recognition properties.. Scientific reports, (21): [PMID:28827714]
13. Goepfert, Arnaud; Lehmann, Sylvie; Blank, Jutta; Kolbinger, Frank and Rondeau, Jean-Michel.. (2020) Structural Analysis Reveals that the Cytokine IL-17F Forms a Homodimeric Complex with Receptor IL-17RC to Drive IL-17RA-Independent Signaling.. Immunity, (17): [PMID:32187518]
14. Gordon, David E and 199 more authors.. (2020) Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms.. Science (New York, N.Y.), (4): [PMID:33060197]

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