Recombinant Human IL-17F Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: Q96PD4
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp147435-10μg	10μg	现货
rp147435-50μg	50μg	现货
rp147435-100μg	100μg	现货
rp147435-1mg	1mg	期货

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点击查看蛋白信息: IL17F

Accession#:Q96PD4 (3) Gene ID:112744 (3) IL17F (3)

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基本描述

产品名称	Recombinant Human IL-17F Protein
别名	重组人白介素17F蛋白 \| 重组人 IL-17F 蛋白
英文别名	Cytokine ML-1 \| IL17F \| IL-17F \| IL-17Finterleukin-17F \| interleukin 17F \| ML1 \| ML-1 \| IL17A \| CANDF6 \| Cytokine ML-1 \| IL-17F
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity >95% by SDS-PAGE and HPLC analyses. Function Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/ml, corresponding to a specific activity of > 5.0 × 104 IU/mg.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	31-163 aa
序列	MRKIPKVGHTFFQKPESCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHVQ
纯度	＞95%(SDS-PAGE, HPLC)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
Accession #	Q96PD4
来源	重组表达
预测分子量	30.1 kDa
SDS-PAGE	16.4 kDa, under reducing conditions.

储存与运输

应用	Cell Culture,Active
物理形态	冻干(Lyophilized)
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 6 mM HCl to a concentration of 0.1mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	使用手动除霜冰柜，避免反复冻融循环。自收到之日起 12 个月，-20 至 -70 °C。复溶后 1 个月，无菌条件下 2 至 8 °C。3 个月，复溶后在无菌条件下 -20 至 -70 °C。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-17F Protein (rp147435)

Recombinant Human IL-17F Protein (rp147435)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 20 ng/mL, corresponding to a specific activity of > 5.0 × 10⁴ IU/mg.

SDS-PAGE: Recombinant Human IL-17F Protein (rp147435)

Recombinant Human IL-17F Protein (rp147435)-SDS-PAGE
Recombinant Human IL-17F Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining. Showing a single band at 16.4 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600465	分析证书	rp147435
ZJ23F0600463	分析证书	rp147435
ZJ23F0600464	分析证书	rp147435

技术文档和文章

引用文献

1. Wright JF, Bennett F, Li B, Brooks J, Luxenberg DP, Whitters MJ, Tomkinson KN, Fitz LJ, Wolfman NM, Collins M et al.. (2008) The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex.. J Immunol, 181 (4): (2799-805). [PMID:18684971]
2. Hymowitz, S G SG and 13 more authors.. (2001) IL-17s adopt a cystine knot fold: structure and activity of a novel cytokine, IL-17F, and implications for receptor binding.. The EMBO journal, (1): [PMID:11574464]
3. Starnes, T T and 6 more authors.. (2001) Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:11591732]
4. Kawaguchi, M M and 9 more authors.. (2001) Identification of a novel cytokine, ML-1, and its expression in subjects with asthma.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:11591768]
5. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
6. Wright, Jill F JF and 12 more authors.. (2007) Identification of an interleukin 17F/17A heterodimer in activated human CD4+ T cells.. The Journal of biological chemistry, (4): [PMID:17355969]
7. Kuestner, Rolf E RE and 27 more authors.. (2007) Identification of the IL-17 receptor related molecule IL-17RC as the receptor for IL-17F.. Journal of immunology (Baltimore, Md. : 1950), (15): [PMID:17911633]
8. Ely, Lauren K LK, Fischer, Suzanne S and Garcia, K Christopher KC.. (2009) Structural basis of receptor sharing by interleukin 17 cytokines.. Nature immunology, [PMID:19838198]
9. Puel, Anne A and 23 more authors.. (2011) Chronic mucocutaneous candidiasis in humans with inborn errors of interleukin-17 immunity.. Science (New York, N.Y.), (1): [PMID:21350122]
10. Goepfert, Arnaud A, Lehmann, Sylvie S, Wirth, Emmanuelle E and Rondeau, Jean-Michel JM.. (2017) The human IL-17A/F heterodimer: a two-faced cytokine with unique receptor recognition properties.. Scientific reports, (21): [PMID:28827714]
11. Goepfert, Arnaud; Lehmann, Sylvie; Blank, Jutta; Kolbinger, Frank and Rondeau, Jean-Michel.. (2020) Structural Analysis Reveals that the Cytokine IL-17F Forms a Homodimeric Complex with Receptor IL-17RC to Drive IL-17RA-Independent Signaling.. Immunity, (17): [PMID:32187518]

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