Recombinant Human IL-10 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: P22301
蛋白标签: No tag
生物活性: 1. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED₅₀ for this effect is typically 1.0 - 8.0 ng/mL. 2. Immobilized Recombinant Human IL-10 Protein (rp147355) at 2 μg/mL can bind Recombinant Rhesus IL10RA Protein (Fc Tag) the EC₅₀ for this effect is 100.0 - 300.0 ng/mL.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp147355-10μg	10μg	期货
rp147355-50μg	50μg	期货
rp147355-100μg	100μg	现货
rp147355-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL10

Accession#:P22301 (8) Gene ID:3586 (8) IL10 (9)

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基本描述

产品名称	Recombinant Human IL-10 Protein
别名	重组人IL-10蛋白 \| 重组人白介素10 \| 重组人 IL-10 蛋白
英文别名	CSIF \| CSIFMGC126450 \| Cytokine synthesis inhibitory factor \| GVHDS \| IL10 \| IL-10 \| IL10A \| IL-10MGC126451 \| interleukin 10 \| interleukin-10 \| TGIF \| CSIF \| Cytokine synthesis inhibitory factor \| IL-10
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity >95% (SDS-PAGE) Endotoxin level <1.0 EU/μg Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.
生物活性	1. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED₅₀ for this effect is typically 1.0 - 8.0 ng/mL. 2. Immobilized Recombinant Human IL-10 Protein (rp147355) at 2 μg/mL can bind Recombinant Rhesus IL10RA Protein (Fc Tag) the EC₅₀ for this effect is 100.0 - 300.0 ng/mL.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	19-178 aa
序列	SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
纯度	>95% (SDS-PAGE)
蛋白标签	No tag
无载体	Yes
无动物源	Yes
Accession #	P22301
来源	重组表达
预测分子量	18.6 KDa
SDS-PAGE	15.9 kDa, under reducing conditions; 14.7 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from sterile 1*PBS, 5% Trehalose, 5% Mannitol.
复溶	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	样品自收到之日起在 -20℃ 至 -80℃ 下可稳定保存 12 个月。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IL-10 Protein (rp147355)

Recombinant Human IL-10 Protein (rp147355) - Protein Bioactivity
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED₅₀ for this effect is typically 1.0 - 8.0 ng/mL.

Protein Bioactivity: Recombinant Human IL-10 Protein (rp147355)

Recombinant Human IL-10 Protein (rp147355) - Protein Bioactivity
Immobilized Recombinant Human IL-10 Protein (rp147355) at 2 μg/mL can bind Recombinant Rhesus IL10RA Protein (Fc Tag) the EC₅₀ for this effect is 100.0 - 300.0 ng/mL.

SDS-PAGE: Recombinant Human IL-10 Protein (rp147355)

Recombinant Human IL-10 Protein (rp147355) - SDS-PAGE
3 μg/lane of Recombinant Human IL-10 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 15.9 kDa under reducing conditions and 14.7 kDa under non-reducing conditions.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404856	分析证书	rp147355
ZJ24F0404857	分析证书	rp147355
ZJ24F0404858	分析证书	rp147355

技术文档和文章

引用文献

1. Vieira, P P and 9 more authors.. (1991) Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: homology to Epstein-Barr virus open reading frame BCRFI.. Proceedings of the National Academy of Sciences of the United States of America, (15): [PMID:1847510]
2. de Waal Malefyt, R R, Abrams, J J, Bennett, B B, Figdor, C G CG and de Vries, J E JE.. (1991) Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes.. The Journal of experimental medicine, (1): [PMID:1940799]
3. Walter, M R MR and Nagabhushan, T L TL.. (1995) Crystal structure of interleukin 10 reveals an interferon gamma-like fold.. Biochemistry, (26): [PMID:7547951]
4. Windsor, W T WT and 9 more authors.. (1993) Disulfide bond assignments and secondary structure analysis of human and murine interleukin 10.. Biochemistry, (31): [PMID:8364028]
5. Zdanov, A A and 5 more authors.. (1995) Crystal structure of interleukin-10 reveals the functional dimer with an unexpected topological similarity to interferon gamma.. Structure (London, England : 1993), (15): [PMID:8590020]
6. Zdanov, A A, Schalk-Hihi, C C and Wlodawer, A A.. (1996) Crystal structure of human interleukin-10 at 1.6 A resolution and a model of a complex with its soluble receptor.. Protein science : a publication of the Protein Society, [PMID:8897595]
7. Gesser, B B and 8 more authors.. (1997) Identification of functional domains on human interleukin 10.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:9405662]
8. Josephson, K K, Logsdon, N J NJ and Walter, M R MR.. (2001) Crystal structure of the IL-10/IL-10R1 complex reveals a shared receptor binding site.. Immunity, [PMID:11485736]
9. van der Linde, K K and 6 more authors.. (2003) A Gly15Arg mutation in the interleukin-10 gene reduces secretion of interleukin-10 in Crohn disease.. Scandinavian journal of gastroenterology, [PMID:12825869]
10. Lin, Ming-Tseh MT and 6 more authors.. (2003) Relation of an interleukin-10 promoter polymorphism to graft-versus-host disease and survival after hematopoietic-cell transplantation.. The New England journal of medicine, (4): [PMID:14657427]
11. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
12. Yoon, Sung Il SI, Jones, Brandi C BC, Logsdon, Naomi J NJ and Walter, Mark R MR.. (2005) Same structure, different function crystal structure of the Epstein-Barr virus IL-10 bound to the soluble IL-10R1 chain.. Structure (London, England : 1993), [PMID:15837194]
13. Yoon, Sung Il SI, Logsdon, Naomi J NJ, Sheikh, Faruk F, Donnelly, Raymond P RP and Walter, Mark R MR.. (2006) Conformational changes mediate interleukin-10 receptor 2 (IL-10R2) binding to IL-10 and assembly of the signaling complex.. The Journal of biological chemistry, (17): [PMID:16982608]
14. El Kasmi, Karim C KC and 9 more authors.. (2007) Cutting edge: A transcriptional repressor and corepressor induced by the STAT3-regulated anti-inflammatory signaling pathway.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:18025162]
15. Sundberg, Thomas B TB and 16 more authors.. (2016) Development of Chemical Probes for Investigation of Salt-Inducible Kinase Function in Vivo.. ACS chemical biology, (19): [PMID:27224444]

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Recombinant Human IL-10 Protein

库存信息

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库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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引用文献

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