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Recombinant Human IL1 Receptor I/IL-1R-1 Protein

Recombinant Human IL1 Receptor I/IL-1R-1 Protein

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P14778
蛋白标签: C-His
生物活性: Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.2-1.0 µg/mL with human IL-1 beta.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp147353-10μg	10μg	现货
rp147353-50μg	50μg	现货
rp147353-100μg	100μg	现货
rp147353-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IL1R1

Accession#:P14778 (3) Gene ID:3554 (3) Il1r1 (4)

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基本描述

产品名称	Recombinant Human IL1 Receptor I/IL-1R-1 Protein
别名	重组人 IL1 受体 I/IL-1R-1 蛋白
英文别名	CD121 antigen-like family member A \| EC 3.2.2.6 \| IL-1R-1 \| IL-1R-alpha \| IL-1RT1 \| IL-1RT-1 \| Interleukin-1 receptor alpha \| Interleukin-1 receptor type I \| p80
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity >95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Receptor for interleukin-1 alpha (IL-1A), beta (IL-1B), and interleukin-1 receptor antagonist protein (IL-1RA). Binding to the agonist leads to the activation of NF-kappa-B. Signaling involves formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2.
生物活性	Measured by its ability to inhibit proliferation using D10.G4.1 mouse helper T cells. The ED50 for this effect is 0.2-1.0 µg/mL with human IL-1 beta.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	21-332 aa
序列	DKCKEREEKIILVSSANEIDVRPCPLNPNEHKGTITWYKDDSKTPVSTEQASRIHQHKEKLWFVPAKVEDSGHYYCVVRNSSYCLRIKISAKFVENEPNLCYNAQAIFKQKLPVAGDGGLVCPYMEFFKNENNELPKLQWYKDCKPLLLDNIHFSGVKDRLIVMNVAEKHRGNYTCHASYTYLGKQYPITRVIEFITLEENKPTRPVIVSPANETMEVDLGSQIQLICNVTGQLSDIAYWKWNGSVIDEDDPVLGEDYYSVENPANKRRSTLITVLNISEIESRFYKHPFTCFAKNTHGIDAAYIQLIYPVTHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P14778
来源	重组表达
预测分子量	55 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% trehalose, 5% mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

SDS-PAGE: Recombinant Human IL1 Receptor I/IL-1R-1 Protein (rp147353)

Recombinant Human IL1 Receptor I/IL-1R-1 Protein (rp147353) - SDS-PAGE
3 μg/lane of Recombinant Human IL1 Receptor I/IL-1R-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 50 - 60 kDa under reducing condition and 45 - 55 kDa under non-reducing condition.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0505465	分析证书	rp147353
ZJ24F0505463	分析证书	rp147353
ZJ24F0505464	分析证书	rp147353

技术文档和文章

引用文献

1. Dripps DJ, Verderber E, Ng RK, Thompson RC, Eisenberg SP. (1991) Interleukin-1 receptor antagonist binds to the type II interleukin-1 receptor on B cells and neutrophils.. J Biol Chem, 266 (30): (20311-5). [PMID:1834644]
2. Schreuder H, Tardif C, Trump-Kallmeyer S, Soffientini A, Sarubbi E, Akeson A, Bowlin T, Yanofsky S, Barrett RW. (1997) A new cytokine-receptor binding mode revealed by the crystal structure of the IL-1 receptor with an antagonist.. Nature, 386 (6621): (194-200). [PMID:9062194]
3. Haile PA, Votta BJ, Marquis RW, Bury MJ, Mehlmann JF, Singhaus Jr R, Charnley AK, Lakdawala AS, Convery MA, Lipshutz DB et al.. (2016) The Identification and Pharmacological Characterization of 6-(tert-Butylsulfonyl)-N-(5-fluoro-1H-indazol-3-yl)quinolin-4-amine (GSK583), a Highly Potent and Selective Inhibitor of RIP2 Kinase.. J Med Chem, 59 (10): (4867-80). [PMID:27109867]
4. Kovalchin J, King B, Masci A, Hopkins E, Fry J, Hou J, Li C, Tenneson K, Weber S, Wolfe G et al.. (2018) Preclinical Development of EBI-005: An IL-1 Receptor-1 Inhibitor for the Topical Ocular Treatment of Ocular Surface Inflammatory Diseases.. Eye Contact Lens, 44 (3): (170-181). [PMID:28727604]
5. Giri JG, Wells J, Dower SK, McCall CE, Guzman RN, Slack J, Bird TA, Shanebeck K, Grabstein KH, Sims JE et al.. (1994) Elevated levels of shed type II IL-1 receptor in sepsis. Potential role for type II receptor in regulation of IL-1 responses.. J Immunol, 153 (12): (5802-9). [PMID:7989776]
6. Haile PA, Casillas LN, Votta BJ, Wang GZ, Charnley AK, Dong X, Bury MJ, Romano JJ, Mehlmann JF, King BW et al.. (2019) Discovery of a First-in-Class Receptor Interacting Protein 2 (RIP2) Kinase Specific Clinical Candidate, 2-((4-(Benzo[d]thiazol-5-ylamino)-6-(tert-butylsulfonyl)quinazolin-7-yl)oxy)ethyl Dihydrogen Phosphate, for the Treatment of Inflammatory Diseases.. J Med Chem, 62 (14): (6482-6494). [PMID:31265286]
7. Zhang L, Shi L, Soars SM, Kamps J, Yin H.. (2018) Discovery of Novel Small-Molecule Inhibitors of NF-κB Signaling with Antiinflammatory and Anticancer Properties.. J Med Chem, 61 (14): (5881-5899). [PMID:29870245] [10.1021/acs.jmedchem.7b01557]
8. Knoepfel T, Nimsgern P, Jacquier S, Bourrel M, Vangrevelinghe E, Glatthar R, Behnke D, Alper PB, Michellys PY, Deane J, Junt T, Zipfel G, Limonta S, Hawtin S, Andre C, Boulay T, Loetscher P, Faller M, Blank J, Feifel R, Betschart C.. (2020) Target-Based Identification and Optimization of 5-Indazol-5-yl Pyridones as Toll-like Receptor 7 and 8 Antagonists Using a Biochemical TLR8 Antagonist Competition Assay.. J Med Chem, 63 (15): (8276-8295). [PMID:32786235] [10.1021/acs.jmedchem.0c00130]
9. Sims, J E JE and 6 more authors.. (1989) Cloning the interleukin 1 receptor from human T cells.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2530587]
10. Chua, A O AO and Gubler, U U.. (1989) Sequence of the cDNA for the human fibroblast type interleukin-1 receptor.. Nucleic acids research, (11): [PMID:2532321]
11. Vigers, G P GP, Anderson, L J LJ, Caffes, P P and Brandhuber, B J BJ.. (1997) Crystal structure of the type-I interleukin-1 receptor complexed with interleukin-1beta.. Nature, (13): [PMID:9062193]
12. Huang, J J, Gao, X X, Li, S S and Cao, Z Z.. (1997) Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein.. Proceedings of the National Academy of Sciences of the United States of America, (25): [PMID:9371760]
13. Vannier, E E and 6 more authors.. (1999) Elevated circulating levels of soluble interleukin-1 receptor type II during interleukin-2 immunotherapy.. European cytokine network, [PMID:10210771]
14. Tominaga, K K and 7 more authors.. (2000) IL-12 synergizes with IL-18 or IL-1beta for IFN-gamma production from human T cells.. International immunology, [PMID:10653850]
15. Vigers, G P GP, Dripps, D J DJ, Edwards, C K CK and Brandhuber, B J BJ.. (2000) X-ray crystal structure of a small antagonist peptide bound to interleukin-1 receptor type 1.. The Journal of biological chemistry, (24): [PMID:10903327]
16. Chen, X X, Ji, Z L ZL and Chen, Y Z YZ.. (2002) TTD: Therapeutic Target Database.. Nucleic acids research, (1): [PMID:11752352]
17. Vamvakopoulos, Joannis J, Green, Colin C and Metcalfe, Su S.. (2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.. European journal of immunology, [PMID:12355453]
18. and Dayer, J M JM.. (2003) The pivotal role of interleukin-1 in the clinical manifestations of rheumatoid arthritis.. Rheumatology (Oxford, England), [PMID:12817089]
19. Hillier, Ladeana W LW and 121 more authors.. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4.. Nature, (7): [PMID:15815621]
20. Do, Herve H and 12 more authors.. (2006) Exhaustive genotyping of the interleukin-1 family genes and associations with AIDS progression in a French cohort.. The Journal of infectious diseases, (1): [PMID:17083033]
21. So, Alexander A, De Smedt, Thibaut T, Revaz, Sylvie S and Tschopp, Jürg J.. (2007) A pilot study of IL-1 inhibition by anakinra in acute gout.. Arthritis research & therapy, [PMID:17352828]
22. Tang, Ying-hong YH, Zhang, Shu-ping SP, Liang, Yan Y and Deng, Chang-qing CQ.. (2007) [Effects of Panax notoginseng saponins on mRNA expressions of interleukin-1 beta, its correlative factors and cysteinyl-aspartate specific protease after cerebral ischemia-reperfusion in rats].. Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine, [PMID:17498496]

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