Recombinant Human IGFBP5 Protein

COA

级别和纯度:

≥96%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: P24593
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
内毒素水平: <0.1 EU/μg

有货

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货号 (SKU)	包装规格	是否现货
rp147287-10µg	10μg	现货
rp147287-50µg	50μg	现货
rp147287-100µg	100μg	现货
rp147287-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IGFBP5

Accession#:P24593 (1) Gene ID:3488 (1) IGFBP5 (2)

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基本描述

产品名称	Recombinant Human IGFBP5 Protein
别名	重组人IGFBP5蛋白 \| 重组人 IGFBP5 蛋白
英文别名	IBP5IBP-5 \| IGF-binding protein 5 \| IGFBP5 \| IGFBP-5 \| Insulin-like growth factor binding protein 5 \| Insulin-like growth factor-binding protein 5 \| IBP 5 \| IBP-5 \| IBP5 \| IBP5_HUMAN \| IGF binding protein 5 \| IGF BP5 \| IBP-5 \| IGF-binding protein 5 \| IGFB
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥96%(SDS-PAGE&HPLC)
产品介绍	Purity >96% SDS-PAGE and HPLC analyses. Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
生物活性	Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/ml, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/ml of rHuIGF-II.
内毒素水平	<0.1 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	21-272aa
序列	LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE
纯度	＞96%(SDS-PAGE and HPLC)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
Accession #	P24593
来源	重组表达
预测分子量	28.6 kDa
SDS-PAGE	28.6 kDa

储存与运输

应用	Cell Culture,WB,Active,SDS-PAGE
物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 3.0
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	收货后建议分装，储存于 -20°C，避免冷冻/解冻循环。冻干，无添加剂。本品为活性蛋白质，可能会引起体内生物反应，请谨慎处理。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IGFBP5 Protein (rp147287)

Recombinant Human IGFBP5 Protein (rp147287)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to inhibit IGF-II induced proliferation of MCF-7 cells is less than 0.4 μg/mL, corresponding to a specific activity of > 2500 IU/mg in the presence of 15 ng/mL of rHuIGF-II.

SDS-PAGE: Recombinant Human IGFBP5 Protein (rp147287)

Recombinant Human IGFBP5 Protein (rp147287)-SDS-PAGE
Recombinant Human IGFBP5 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.6 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600315	分析证书	rp147287
ZJ23F0600316	分析证书	rp147287
ZJ23F0600314	分析证书	rp147287

技术文档和文章

引用文献

1. Shimasaki, S S, Shimonaka, M M, Zhang, H P HP and Ling, N N.. (1991) Identification of five different insulin-like growth factor binding proteins (IGFBPs) from adult rat serum and molecular cloning of a novel IGFBP-5 in rat and human.. The Journal of biological chemistry, (5): [PMID:1709938]
2. Andress, D L DL and Birnbaum, R S RS.. (1991) A novel human insulin-like growth factor binding protein secreted by osteoblast-like cells.. Biochemical and biophysical research communications, (15): [PMID:1850257]
3. Kiefer, M C MC, Ioh, R S RS, Bauer, D M DM and Zapf, J J.. (1991) Molecular cloning of a new human insulin-like growth factor binding protein.. Biochemical and biophysical research communications, (15): [PMID:1850258]
4. Allander, S V SV and 9 more authors.. (1994) Characterization of the chromosomal gene and promoter for human insulin-like growth factor binding protein-5.. The Journal of biological chemistry, (8): [PMID:7511611]
5. Kalus, W W and 10 more authors.. (1998) Structure of the IGF-binding domain of the insulin-like growth factor-binding protein-5 (IGFBP-5): implications for IGF and IGF-I receptor interactions.. The EMBO journal, (16): [PMID:9822601]
6. Ständker, L L, Wobst, P P, Mark, S S and Forssmann, W G WG.. (1998) Isolation and characterization of circulating 13-kDa C-terminal fragments of human insulin-like growth factor binding protein-5.. FEBS letters, (18): [PMID:9883900]
7. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]

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Recombinant Human IGFBP5 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

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引用文献

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