Recombinant Human IGFBP3 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P17936
蛋白标签: C-His
生物活性: Immobilized Human IGFBP3 Protein (rp147276) at 1.0 μg/mL can bind IGF-1 with a linear range of 0.7 - 50.0 ng/mL. The EC50 for this effect is 73.39 ng/mL.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp147276-10μg	10μg	现货
rp147276-50μg	50μg	现货
rp147276-100μg	100μg	现货
rp147276-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: IGFBP3

Accession#:P17936 (2) Gene ID:3486 (2) IGFBP3 (2)

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基本描述

产品名称	Recombinant Human IGFBP3 Protein
别名	重组人IGFBP3蛋白 \| 重组人IGFBP3蛋白 \| 重组人 IGFBP3 蛋白
英文别名	acid stable subunit of the 140 K IGF complex \| binding protein 29 \| binding protein 53 \| growth hormone-dependent binding protein \| IBP-3 \| IBP3BP-53 \| IGF-binding protein 3 \| IGFBP3 \| IGFBP-3 \| insulin-like growth factor binding protein 3 \| insulin-like
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% (SDS-PAGE) Endotoxin level ＜0.1 EU/μg Function IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
生物活性	Immobilized Human IGFBP3 Protein (rp147276) at 1.0 μg/mL can bind IGF-1 with a linear range of 0.7 - 50.0 ng/mL. The EC50 for this effect is 73.39 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	28-291 aa
序列	GASSAGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGSVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKRGFCWCVDKYGQPLPGYTTKGKEDVHCYSMQSKHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
偶联	Unconjugated
Accession #	P17936
来源	重组表达
预测分子量	29.7 kDa
SDS-PAGE	40.0 - 50.0 kDa, under reducing conditions; 40.0 - 50.0 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human IGFBP3 Protein (rp147276)

Recombinant Human IGFBP3 Protein (rp147276) - Protein Bioactivity
Immobilized Human IGFBP3 Protein (rp147276) at 1.0 μg/mL can bind IGF-1 with a linear range of 0.7 - 50.0 ng/mL. The EC₅₀ for this effect is 73.39 ng/mL.

SDS-PAGE: Recombinant Human IGFBP3 Protein (rp147276)

Recombinant Human IGFBP3 Protein (rp147276) - SDS-PAGE
3 μg/lane of Recombinant Human IGFBP3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 40.0 - 50.0 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0607348	分析证书	rp147276
ZJ24F0607346	分析证书	rp147276
ZJ24F0607347	分析证书	rp147276

技术文档和文章

引用文献

1. Scherer SW, Cheung J, MacDonald JR, Osborne LR, Nakabayashi K, Herbrick JA, Carson AR, Parker-Katiraee L, Skaug J, Khaja R et al.. (2003) Human chromosome 7: DNA sequence and biology.. Science, 300 (5620): (767-72). [PMID:12690205]
2. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
3. Evert Njomen,Hedeel G Evans,Samanthi H Gedara,Deborah L Heyl. (2015-07-29) Humanin Peptide Binds to Insulin-Like Growth Factor-Binding Protein 3 (IGFBP3) and Regulates Its Interaction with Importin-β.. Protein and peptide letters, 22 ((10)): (869-876). [PMID:26216267]
4. Cubbage, M L ML, Suwanichkul, A A and Powell, D R DR.. (1990) Insulin-like growth factor binding protein-3. Organization of the human chromosomal gene and demonstration of promoter activity.. The Journal of biological chemistry, (25): [PMID:1695633]
5. Zapf, J J and 7 more authors.. (1990) Isolation from adult human serum of four insulin-like growth factor (IGF) binding proteins and molecular cloning of one of them that is increased by IGF I administration and in extrapancreatic tumor hypoglycemia.. The Journal of biological chemistry, (5): [PMID:1697583]
6. Roghani, M M, Segovia, B B, Whitechurch, O O and Binoux, M M.. (1991) Purification from human cerebrospinal fluid of insulin-like growth factor binding proteins (IGFBPs). Isolation of IGFBP-2, an altered form of IGFBP-3 and a new IGFBP species.. Growth regulation, [PMID:1726837]
7. Wood, W I WI and 7 more authors.. (1988) Cloning and expression of the growth hormone-dependent insulin-like growth factor-binding protein.. Molecular endocrinology (Baltimore, Md.), [PMID:2464130]
8. Thweatt, R R, Fleischmann, R R and Goldstein, S S.. (1993) Analysis of the primary structure of insulin-like growth factor binding protein-3 cDNA from Werner syndrome fibroblasts.. DNA sequence : the journal of DNA sequencing and mapping, [PMID:7508771]
9. Hillier, Ladeana W LW and 106 more authors.. (2003) The DNA sequence of human chromosome 7.. Nature, (10): [PMID:12853948]
10. Ikonen, Maaria M and 7 more authors.. (2003) Interaction between the Alzheimer's survival peptide humanin and insulin-like growth factor-binding protein 3 regulates cell survival and apoptosis.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:14561895]
11. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR.. (2004) Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.. Proteomics, [PMID:14760718]
12. Zhang, Zemin Z and Henzel, William J WJ.. (2004) Signal peptide prediction based on analysis of experimentally verified cleavage sites.. Protein science : a publication of the Protein Society, [PMID:15340161]
13. (2005) Mecasermin rinfabate: insulin-like growth factor-I/insulin-like growth factor binding protein-3, mecaserimin rinfibate, rhIGF-I/rhIGFBP-3.. Drugs in R&D, [PMID:15777106]
14. Liu, Tao T and 6 more authors.. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
15. Chang, Yoon Soo YS and 7 more authors.. (2006) 9-cis retinoic acid induces insulin-like growth factor binding protein-3 through DR-8 retinoic acid responsive elements.. Cancer biology & therapy, [PMID:16760641]
16. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
17. Williams, Rachel M RM, McDonald, Anna A, O'Savage, Martin M and Dunger, David B DB.. (2008) Mecasermin rinfabate: rhIGF-I/rhIGFBP-3 complex: iPLEX.. Expert opinion on drug metabolism & toxicology, [PMID:18363546]
18. Muzumdar, Radhika H RH and 12 more authors.. (2009) Humanin: a novel central regulator of peripheral insulin action.. PloS one, (22): [PMID:19623253]
19. and Kemp, Stephen F SF.. (2009) Insulin-like growth factor-I deficiency in children with growth hormone insensitivity: current and future treatment options.. BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy, [PMID:19627167]
20. Lue, YanHe Y and 10 more authors.. (2010) Opposing roles of insulin-like growth factor binding protein 3 and humanin in the regulation of testicular germ cell apoptosis.. Endocrinology, [PMID:19952275]
21. Ingermann, Angela R AR and 11 more authors.. (2010) Identification of a novel cell death receptor mediating IGFBP-3-induced anti-tumor effects in breast and prostate cancer.. The Journal of biological chemistry, (24): [PMID:20353938]
22. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]

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