Recombinant Human G-CSF Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: P09919-2
蛋白标签: C-His
生物活性: Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.
内毒素水平: <0.1 EU/μg

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货号 (SKU)	包装规格	是否现货
rp146356-10μg	10μg	现货
rp146356-50μg	50μg	现货
rp146356-100µg	100μg	现货
rp146356-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CSF3

Accession#:P09919 (3) CSF3 (5) G-CSF (5) Gene ID:1440 (3)

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基本描述

产品名称	Recombinant Human G-CSF Protein
别名	重组人G-CSF蛋白 \| 重组人粒细胞集落刺激因子
英文别名	C17orf33 \| chromosome 17 open reading frame 33 \| colony stimulating factor 3 (granulocyte) \| CSF3 \| CSF3OS \| Filgrastim \| GCSF \| G-CSF \| GCSFlenograstim \| granulocyte colony-stimulating factor \| Lenograstim \| MGC45931 \| Pluripoietin
规格或纯度	无动物源, 无载体, 生物活性, ActiBioPure™, 高性能, ≥95%(SDS-PAGE)
生化机理	Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Post-translational: O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).
生物活性	Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	1-204 aa
序列	MAGPATQSPMKLMALQLLLWHSALWTVQEATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQPHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
蛋白长度	Protein fragment
无载体	Yes
无动物源	Yes
Accession #	P09919-2
来源	重组表达
预测分子量	23 kDa
SDS-PAGE	17.5 kDa, under reducing conditions; 17.5 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% trehalose, 5% mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human G-CSF Protein (rp146356)

Recombinant Human G-CSF Protein (rp146356) - Protein Bioactivity
Measured in a cell proliferation assay using NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 1.05 pg/mL.

SDS-PAGE: Recombinant Human G-CSF Protein (rp146356)

Recombinant Human G-CSF Protein (rp146356) - SDS-PAGE
3 μg/lane of Recombinant Human G-CSF Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 17.5 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到5个结果

批号(Lot Number)	证书类型	货号
ZJ24F0607422	分析证书	rp146356
ZJ24F0607423	分析证书	rp146356
ZJ24F0607424	分析证书	rp146356
ZJ24F1214960	分析证书	rp146356
ZJ24F1214958	分析证书	rp146356

技术文档和文章

引用文献

1. Tamada T, Honjo E, Maeda Y, Okamoto T, Ishibashi M, Tokunaga M, Kuroki R. (2006) Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex.. Proc Natl Acad Sci USA, 103 (9): (3135-40). [PMID:16492764]
2. Zink, T T, Ross, A A, Ambrosius, D D, Rudolph, R R and Holak, T A TA.. (1992) Secondary structure of human granulocyte colony-stimulating factor derived from NMR spectroscopy.. FEBS letters, (21): [PMID:1281794]
3. Souza, L M LM and 14 more authors.. (1986) Recombinant human granulocyte colony-stimulating factor: effects on normal and leukemic myeloid cells.. Science (New York, N.Y.), (4): [PMID:2420009]
4. Nagata, S S and 8 more authors.. (1986) The chromosomal gene structure and two mRNAs for human granulocyte colony-stimulating factor.. The EMBO journal, [PMID:2423327]
5. Nagata, S S and 9 more authors.. (1986) Molecular cloning and expression of cDNA for human granulocyte colony-stimulating factor.. Nature, [PMID:3484805]
6. Devlin, J J JJ and 5 more authors.. (1987) Expression of granulocyte colony-stimulating factor by human cell lines.. Journal of leukocyte biology, [PMID:3494801]
7. Zink, T T and 5 more authors.. (1994) Structure and dynamics of the human granulocyte colony-stimulating factor determined by NMR spectroscopy. Loop mobility in a four-helix-bundle protein.. Biochemistry, (19): [PMID:7518249]
8. Hill, C P CP, Osslund, T D TD and Eisenberg, D D.. (1993) The structure of granulocyte-colony-stimulating factor and its relationship to other growth factors.. Proceedings of the National Academy of Sciences of the United States of America, (1): [PMID:7685117]
9. Haniu, M M and 5 more authors.. (1995) Extracellular domain of granulocyte-colony stimulating factor receptor. Interaction with its ligand and identification of a domain in close proximity of ligand-binding region.. Archives of biochemistry and biophysics, (20): [PMID:8554326]
10. Aritomi, M M and 5 more authors.. (1999) Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme.. Nature, (14): [PMID:10537111]
11. Jin, Pei P and 7 more authors.. (2004) PCR isolation and cloning of novel splice variant mRNAs from known drug target genes.. Genomics, [PMID:15028279]
12. Zody, Michael C MC and 73 more authors.. (2006) DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.. Nature, (20): [PMID:16625196]

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