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Recombinant Human Fibronectin Fragment Protein

Recombinant Human Fibronectin Fragment Protein

级别和纯度:

≥98%(SDS-PAGE)

表达系统: E. coli
Accession #: P02751
蛋白标签: C-His
生物活性: It has the effect of promoting cell adhesion. Depending on the cell type, the optimal concentration is 0.2-1.5 μg/cm2
内毒素水平: <10 EU/mg

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货号 (SKU)	包装规格	是否现货
rp146053-1mg	1mg	现货
rp146053-5mg	5mg	现货
rp146053-10mg	10mg	现货

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点击查看蛋白信息: FN1

Accession#:P02751 (3) Fibronectin (3) Fibronectin Fragment (1) FN1 (3) Gene ID:2335 (3)

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基本描述

产品名称	Recombinant Human Fibronectin Fragment Protein
别名	重组人粘连蛋白片段 \| 重组人Fibronectin Fragment
英文别名	CIG \| Cold-insoluble globulin \| FN \| ED-B \| fibronectin 1 \| Fibronectin \| FINC \| FN1 \| FNZ \| GFND \| GFND2 \| LETS \| MSF \| SMDCF
规格或纯度	无动物源, 无载体, GMP, 生物活性, ActiBioPure™, 无菌, 高性能, ≥98%(SDS-PAGE)
生化机理	Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Acts as a ligand for the LILRB4 receptor, inhibiting FCGR1A/CD64-mediated monocyte activation. Post-translational: Sulfated. It is not known whether both or only one of Thr-2064 and Thr-2065 are/is glycosylated. Forms covalent cross-links mediated by a transglutaminase, such as F13A or TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers (e.g. fibrinogen-fibronectin, collagen-fibronectin heteropolymers). Phosphorylated by FAM20C in the extracellular medium. Proteolytic processing produces the C-terminal NC1 peptide, anastellin.
产品介绍	纤维连接蛋白，也称粘连蛋白，纤连蛋白，英文名称fibronectin（英文缩写FN）是一种高分子质量、多结构域的糖蛋白，在各种细胞-细胞和细胞-基质过程中起着关键作用，如细胞附着、扩散、生长、分化和迁移。它存在于细胞表面，有助于ECM结构。本产品包括细胞结合域，肝磷脂结合域和 CS1 位点三个功能区域。它由 574 个氨基酸组成，分子量 63 kDa。能够大幅地提高逆转录病毒感染哺乳动物细胞的感染效率，其作用原理是：细胞表面 VLA-4 及 VLA-5 可分别与本产品上的 CS-1 位点及细胞结合域结合，逆转录病毒载体可以与肝磷脂结合域结合。在经本产品包被的培养容器表面，细胞与病毒载体以高浓度共存，从而提高基因转染效率。包被在细胞培养皿表面时，显着增强了哺乳动物和昆虫细胞的转染能力。本产品用重组大肠杆菌基因表达生产人粘连蛋白片段，高纯度，高活性，无动物源性污染。 Fibronectin is a high molecular weight, multi-domain glycoprotein that plays a key role in various cell-cell and cell-matrix processes, such as cell attachment, diffusion, growth, differentiation and migration. It exists on the cell surface and contributes to the ECM structure. This product includes three functional regions: cell binding domain, heparin binding domain and CS1 site. It consists of 574 amino acids and has a molecular weight of 63 kDa. It can greatly improve the infection efficiency of retroviral infection of mammalian cells. Its working principle is: VLA-4 and VLA-5 on the cell surface can bind to the CS-1 site and cell binding domain on this product respectively, and the retroviral vector can bind to the heparin binding domain. On the surface of the culture container coated with this product, cells and viral vectors coexist at high concentrations, thereby improving the efficiency of gene transfection. When coated on the surface of the cell culture dish, the transfection ability of mammalian and insect cells is significantly enhanced.
生物活性	It has the effect of promoting cell adhesion. Depending on the cell type, the optimal concentration is 0.2-1.5 μg/cm2
内毒素水平	<10 EU/mg
表达系统	E. coli
种属	人(Human)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P02751
来源	重组表达
预测分子量	63 kDa
SDS-PAGE	63 kDa

关联靶点（人）

FN1 Tchem 纤连蛋白(Fibronectin) (0 活性数据)

点击查看靶蛋白信息： FN1

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	10mM PBS, pH7.4.
复溶	Reconstitute in water to a concentration of 1 mg/mL, for other concentrations, please adjust the concentration of phosphate in the solution by yourself.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20℃~-80℃ (2 years). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
CAS编号和信息	86088-83-7
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human Fibronectin Fragment Protein (rp146053)

Recombinant Human Fibronectin Fragment Protein (rp146053) - Protein Bioactivity
Fibronectin Fragment Protein has the effect of promoting cell adhesion. Measured by the ability of the immobilized protein to support the adhesion of NIH/3T3 mouse embryonic fibroblast cells.

Recombinant Human Fibronectin Fragment Protein (rp146053) - Protein Bioactivity Fibronectin Fragment Protein has the effect of promoting cell adhesion. Measured by the ability of the immobilized protein to support the adhesion of NIH/3T3 mouse embryonic f

Recombinant Human Fibronectin Fragment Protein (rp146053) - SDS-PAGE
2 μg/lane of Recombinant Human Fibronectin Fragment Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 56 kDa.

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批号(Lot Number)	证书类型	货号
ZJ25F0521455	分析证书	rp146053
ZJ25F0521454	分析证书	rp146053
ZJ25F0521453	分析证书	rp146053

引用文献

1. Baron, M M and 5 more authors.. (1992) 1H NMR assignment and secondary structure of the cell adhesion type III module of fibronectin.. Biochemistry, (25): [PMID:1311202]
2. Balbona, K K and 5 more authors.. (1992) Fibulin binds to itself and to the carboxyl-terminal heparin-binding region of fibronectin.. The Journal of biological chemistry, (5): [PMID:1400330]
3. Main, A L AL, Harvey, T S TS, Baron, M M, Boyd, J J and Campbell, I D ID.. (1992) The three-dimensional structure of the tenth type III module of fibronectin: an insight into RGD-mediated interactions.. Cell, (13): [PMID:1423622]
4. Tressel, T T and 6 more authors.. (1991) Human plasma fibronectin. Demonstration of structural differences between the A- and B-chains in the III CS region.. The Biochemical journal, (15): [PMID:2012601]
5. Liu, M C MC, Yu, S S, Sy, J J, Redman, C M CM and Lipmann, F F.. (1985) Tyrosine sulfation of proteins from the human hepatoma cell line HepG2.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:2414772]
6. Zardi, L L and 6 more authors.. (1987) Transformed human cells produce a new fibronectin isoform by preferential alternative splicing of a previously unobserved exon.. The EMBO journal, [PMID:2822387]
7. Umezawa, K K, Kornblihtt, A R AR and Baralle, F E FE.. (1985) Isolation and characterization of cDNA clones for human liver fibronectin.. FEBS letters, (1): [PMID:2989004]
8. Bernard, M P MP, Kolbe, M M, Weil, D D and Chu, M L ML.. (1985) Human cellular fibronectin: comparison of the carboxyl-terminal portion with rat identifies primary structural domains separated by hypervariable regions.. Biochemistry, (21): [PMID:2992573]
9. Kornblihtt, A R AR, Umezawa, K K, Vibe-Pedersen, K K and Baralle, F E FE.. (1985) Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene.. The EMBO journal, [PMID:2992939]
10. Oldberg, A A and Ruoslahti, E E.. (1986) Evolution of the fibronectin gene. Exon structure of cell attachment domain.. The Journal of biological chemistry, (15): [PMID:3003095]
11. Sekiguchi, K K, Klos, A M AM, Kurachi, K K, Yoshitake, S S and Hakomori, S S.. (1986) Human liver fibronectin complementary DNAs: identification of two different messenger RNAs possibly encoding the alpha and beta subunits of plasma fibronectin.. Biochemistry, (26): [PMID:3021206]
12. Owens, R J RJ and Baralle, F E FE.. (1986) Mapping the collagen-binding site of human fibronectin by expression in Escherichia coli.. The EMBO journal, [PMID:3024962]
13. Dean, D C DC, Bowlus, C L CL and Bourgeois, S S.. (1987) Cloning and analysis of the promotor region of the human fibronectin gene.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3031656]
14. Paolella, G G, Henchcliffe, C C, Sebastio, G G and Baralle, F E FE.. (1988) Sequence analysis and in vivo expression show that alternative splicing of ED-B and ED-A regions of the human fibronectin gene are independent events.. Nucleic acids research, (25): [PMID:3375063]
15. Gutman, A A and Kornblihtt, A R AR.. (1987) Identification of a third region of cell-specific alternative splicing in human fibronectin mRNA.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3478690]
16. Garcia-Pardo, A A, Rostagno, A A and Frangione, B B.. (1987) Primary structure of human plasma fibronectin. Characterization of a 38 kDa domain containing the C-terminal heparin-binding site (Hep III site) and a region of molecular heterogeneity.. The Biochemical journal, (1): [PMID:3593230]
17. Gutman, A A, Yamada, K M KM and Kornblihtt, A A.. (1986) Human fibronectin is synthesized as a pre-propolypeptide.. FEBS letters, (20): [PMID:3770189]
18. Vibe-Pedersen, K K, Magnusson, S S and Baralle, F E FE.. (1986) Donor and acceptor splice signals within an exon of the human fibronectin gene: a new type of differential splicing.. FEBS letters, (27): [PMID:3770201]
19. Calaycay, J J and 6 more authors.. (1985) Primary structure of a DNA- and heparin-binding domain (Domain III) in human plasma fibronectin.. The Journal of biological chemistry, (5): [PMID:3900070]
20. Garcia-Pardo, A A, Pearlstein, E E and Frangione, B B.. (1985) Primary structure of human plasma fibronectin. Characterization of a 31,000-dalton fragment from the COOH-terminal region containing a free sulfhydryl group and a fibrin-binding site.. The Journal of biological chemistry, (25): [PMID:4019516]
21. Kornblihtt, A R AR, Vibe-Pedersen, K K and Baralle, F E FE.. (1984) Human fibronectin: molecular cloning evidence for two mRNA species differing by an internal segment coding for a structural domain.. The EMBO journal, [PMID:6200322]
22. Kornblihtt, A R AR, Vibe-Pedersen, K K and Baralle, F E FE.. (1983) Isolation and characterization of cDNA clones for human and bovine fibronectins.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6304699]
23. Garcia-Pardo, A A, Pearlstein, E E and Frangione, B B.. (1983) Primary structure of human plasma fibronectin. The 29,000-dalton NH2-terminal domain.. The Journal of biological chemistry, (25): [PMID:6630202]
24. Oldberg, A A, Linney, E E and Ruoslahti, E E.. (1983) Molecular cloning and nucleotide sequence of a cDNA clone coding for the cell attachment domain in human fibronectin.. The Journal of biological chemistry, (10): [PMID:6688418]
25. Pierschbacher, M D MD, Ruoslahti, E E, Sundelin, J J, Lind, P P and Peterson, P A PA.. (1982) The cell attachment domain of fibronectin. Determination of the primary structure.. The Journal of biological chemistry, (25): [PMID:7050098]
26. Potts, J R JR, Phan, I I, Williams, M J MJ and Campbell, I D ID.. (1995) High-resolution structural studies of the factor XIIIa crosslinking site and the first type 1 module of fibronectin.. Nature structural biology, [PMID:7583666]
27. Rostagno, A A, Williams, M J MJ, Baron, M M, Campbell, I D ID and Gold, L I LI.. (1994) Further characterization of the NH2-terminal fibrin-binding site on fibronectin.. The Journal of biological chemistry, (16): [PMID:7989369]
28. Dickinson, C D CD and 6 more authors.. (1994) Crystal structure of the tenth type III cell adhesion module of human fibronectin.. Journal of molecular biology, (4): [PMID:8120888]
29. Williams, M J MJ and 5 more authors.. (1994) Solution structure of a pair of fibronectin type 1 modules with fibrin binding activity.. Journal of molecular biology, (28): [PMID:8308892]
30. Leahy, D J DJ, Aukhil, I I and Erickson, H P HP.. (1996) 2.0 A crystal structure of a four-domain segment of human fibronectin encompassing the RGD loop and synergy region.. Cell, (12): [PMID:8548820]
31. Sasaki, T T, Brakebusch, C C, Engel, J J and Timpl, R R.. (1998) Mac-2 binding protein is a cell-adhesive protein of the extracellular matrix which self-assembles into ring-like structures and binds beta1 integrins, collagens and fibronectin.. The EMBO journal, (16): [PMID:9501082]
32. Sticht, H H, Pickford, A R AR, Potts, J R JR and Campbell, I D ID.. (1998) Solution structure of the glycosylated second type 2 module of fibronectin.. Journal of molecular biology, (13): [PMID:9514732]
33. Sharma, A A, Askari, J A JA, Humphries, M J MJ, Jones, E Y EY and Stuart, D I DI.. (1999) Crystal structure of a heparin- and integrin-binding segment of human fibronectin.. The EMBO journal, (15): [PMID:10075919]
34. Fattorusso, R R and 5 more authors.. (1999) NMR structure of the human oncofoetal fibronectin ED-B domain, a specific marker for angiogenesis.. Structure (London, England : 1993), (15): [PMID:10196121]
35. Bocquier, A A AA, Potts, J R JR, Pickford, A R AR and Campbell, I D ID.. (1999) Solution structure of a pair of modules from the gelatin-binding domain of fibronectin.. Structure (London, England : 1993), (15): [PMID:10647176]
36. Yi, M M and Ruoslahti, E E.. (2001) A fibronectin fragment inhibits tumor growth, angiogenesis, and metastasis.. Proceedings of the National Academy of Sciences of the United States of America, (16): [PMID:11209058]
37. Pickford, A R AR, Smith, S P SP, Staunton, D D, Boyd, J J and Campbell, I D ID.. (2001) The hairpin structure of the (6)F1(1)F2(2)F2 fragment from human fibronectin enhances gelatin binding.. The EMBO journal, (2): [PMID:11285216]
38. Holmes, A R AR and 6 more authors.. (2001) The pavA gene of Streptococcus pneumoniae encodes a fibronectin-binding protein that is essential for virulence.. Molecular microbiology, [PMID:11580843]
39. Schor, S L SL and Schor, A M AM.. (2001) Phenotypic and genetic alterations in mammary stroma: implications for tumour progression.. Breast cancer research : BCR, [PMID:11737888]
40. Filla, Mark S MS and 6 more authors.. (2002) In vitro localization of TIGR/MYOC in trabecular meshwork extracellular matrix and binding to fibronectin.. Investigative ophthalmology & visual science, [PMID:11773026]
41. Niimi, T T and 7 more authors.. (2001) NMR structure of human fibronectin EDA.. Journal of biomolecular NMR, [PMID:11775745]
42. Tu, Hongmin H and 6 more authors.. (2002) The type XIII collagen ectodomain is a 150-nm rod and capable of binding to fibronectin, nidogen-2, perlecan, and heparin.. The Journal of biological chemistry, (21): [PMID:11956183]
43. Parker, A E AE, Boutell, J J, Carr, A A and Maciewicz, R A RA.. (2002) Novel cartilage-specific splice variants of fibronectin.. Osteoarthritis and cartilage, [PMID:12127832]
44. Di Cesare, Paul E PE and 6 more authors.. (2002) Matrix-matrix interaction of cartilage oligomeric matrix protein and fibronectin.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:12225811]
45. Schwarz-Linek, Ulrich U and 10 more authors.. (2003) Pathogenic bacteria attach to human fibronectin through a tandem beta-zipper.. Nature, (8): [PMID:12736686]
46. Gao, Mu M and 5 more authors.. (2003) Structure and functional significance of mechanically unfolded fibronectin type III1 intermediates.. Proceedings of the National Academy of Sciences of the United States of America, (9): [PMID:14657397]
47. Bunkenborg, Jakob J, Pilch, Bartosz J BJ, Podtelejnikov, Alexandre V AV and Wiśniewski, Jacek R JR.. (2004) Screening for N-glycosylated proteins by liquid chromatography mass spectrometry.. Proteomics, [PMID:14760718]
48. Kato, Seishi S, Ohtoko, Kuniyo K, Ohtake, Hideki H and Kimura, Tomoko T.. (2005) Vector-capping: a simple method for preparing a high-quality full-length cDNA library.. DNA research : an international journal for rapid publication of reports on genes and genomes, (28): [PMID:16106752]
49. Kay, Richard A RA and 6 more authors.. (2005) The expression of migration stimulating factor, a potent oncofetal cytokine, is uniquely controlled by 3'-untranslated region-dependent nuclear sequestration of its precursor messenger RNA.. Cancer research, (1): [PMID:16322219]
50. Liu, Tao T and 6 more authors.. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
51. Maguer-Satta, Véronique V and 6 more authors.. (2006) A novel role for fibronectin type I domain in the regulation of human hematopoietic cell adhesiveness through binding to follistatin domains of FLRG and follistatin.. Experimental cell research, (15): [PMID:16336961]
52. Kuznetsova, Svetlana A SA and 9 more authors.. (2008) TSG-6 binds via its CUB_C domain to the cell-binding domain of fibronectin and increases fibronectin matrix assembly.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:18042364]
53. Castelletti, Federica F and 14 more authors.. (2008) Mutations in FN1 cause glomerulopathy with fibronectin deposits.. Proceedings of the National Academy of Sciences of the United States of America, (19): [PMID:18268355]
54. Han, Guanghui G and 9 more authors.. (2008) Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography.. Proteomics, [PMID:18318008]
55. Bingham, Richard J RJ and 7 more authors.. (2008) Crystal structures of fibronectin-binding sites from Staphylococcus aureus FnBPA in complex with fibronectin domains.. Proceedings of the National Academy of Sciences of the United States of America, (26): [PMID:18713862]
56. Erat, Michèle C MC and 6 more authors.. (2009) Identification and structural analysis of type I collagen sites in complex with fibronectin fragments.. Proceedings of the National Academy of Sciences of the United States of America, (17): [PMID:19251642]
57. You, Ran; Klein, R Matthew; Zheng, Mingzhe and McKeown-Longo, Paula J.. (2009) Regulation of p38 MAP kinase by anastellin is independent of anastellin's effect on matrix fibronectin.. Matrix biology : journal of the International Society for Matrix Biology, [PMID:19379667]
58. Zhou, Tianjun T and 8 more authors.. (2010) Structural analysis of DFG-in and DFG-out dual Src-Abl inhibitors sharing a common vinyl purine template.. Chemical biology & drug design, [PMID:19895503]
59. Graille, Marc M, Pagano, Maurice M, Rose, Thierry T, Ravaux, Michèle Reboud MR and van Tilbeurgh, Herman H.. (2010) Zinc induces structural reorganization of gelatin binding domain from human fibronectin and affects collagen binding.. Structure (London, England : 1993), (9): [PMID:20541508]
60. Rosenow, Anja A and 6 more authors.. (2012) Resveratrol-induced changes of the human adipocyte secretion profile.. Journal of proteome research, (7): [PMID:22905912]
61. Tsui, Irena I, Pan, Carolyn K CK, Rahimy, Ehsan E and Schwartz, Steven D SD.. (2012) Ocriplasmin for vitreoretinal diseases.. Journal of biomedicine & biotechnology, [PMID:23193358]
62. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]
63. Lee, Chae Syng CS and 33 more authors.. (2017) Mutations in Fibronectin Cause a Subtype of Spondylometaphyseal Dysplasia with "Corner Fractures".. American journal of human genetics, (2): [PMID:29100092]
64. Kornblihtt, A R AR, Vibe-Pedersen, K K and Baralle, F E FE.. (1984) Human fibronectin: cell specific alternative mRNA splicing generates polypeptide chains differing in the number of internal repeats.. Nucleic acids research, (25): [PMID:6462919]

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