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Recombinant Human EPO Protein

COA COA
  • 表达系统: CHO
  • Accession #: P01588
  • 蛋白标签: C-hFc
  • 生物活性: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is typically less than 5.0 ng/mL.
  • 内毒素水平: <20 EU/mg
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货号 (SKU) 包装规格 是否现货 价格 数量
rp145653-10μg
 10μg 现货 Stock Image
rp145653-50μg
 50μg 现货 Stock Image
rp145653-100μg
 100μg 现货 Stock Image
rp145653-1mg
 1mg 期货 Stock Image
大包装询价 收藏夹 比较  SDS下载  DATASHEET  质量标准
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点击查看蛋白信息: EPO
Accession#:P01588 (3) EPO (4) Gene ID:2056 (3)
main product photo

基本描述

产品名称 Recombinant Human EPO Protein
别名 重组人EPO蛋白 | 重组人 EPO 蛋白
英文别名 EP | EPO | EPO alpha | Epoetin | Erythropoetin | Erythropoietin precursor | MVCD2 | RhEPO
规格或纯度 ActiBioPure™, Bioactive, GMP, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE&HPLC)
产品介绍

促红细胞生成素（EPO）又称红细胞刺激因子、促红素，是一种人体内源性糖蛋白激素，可刺激红细胞生成。缺氧可刺激促红细胞生成素产生，早已有重组人促红细胞生成素用于临床，用于治疗肾功能不全合并的贫血、获得性免疫缺陷综合征/艾滋病本身或治疗引起的贫血、恶性肿瘤伴发的贫血及风湿病贫血等多种贫血。


本产品系由含有高效表达人EPO-Fc基因的真核表达系统（CHO-k1）经发酵（培养基不含有任何动物源成分）、分离和高度纯化后经冻干制成。由人EPO的166个氨基酸残基（Ala28-Arg193）和人IgG Fc段组成的多肽链，分子量为58kDa。


本产品为无菌冻干粉剂，由含有10mMPBS pH为7.2的蛋白溶液经0.2μm过滤后分装冻干。

Purity

≥ 95% SDS-PAGE; HPLC


Relevance

Human erythropoietin is member of the EPO/TPO family and encodes a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. It is produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.
生物活性 Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is typically less than 5.0 ng/mL.
内毒素水平 <20 EU/mg
表达系统 CHO
种属 人(Human)
氨基酸 28-193 aa
序列 APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR
纯度 ＞95% (SDS-PAGE and HPLC)
蛋白标签 C-hFc
无载体 Yes
Accession # P01588
来源 重组表达
预测分子量 58 kDa
SDS-PAGE 61.8 kDa, under reducing conditions.

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 10mM PBS，5%的甘露醇，0.01%吐温80，pH7.4
复溶 We recommend that this vial be briefly centrifuged prior to opening to bring the contents to thebottom. Reconstitute in sterile water for injection to a concentration of 100μg/mL. Stock solutions should be apportioned into working aliquots and stored at 4℃ for 7-10 days, and long term storage at ≤ -20 °C.
储存温度 -20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 交付后等分，储存于 -20°C，避免冷冻/解冻循环。冻干，无添加剂。本品为活性蛋白质，可能会引起体内生物反应，请谨慎处理。
分子类型 蛋白质

图片

Recombinant Human EPO Protein (rp145653) - Protein Bioactivity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is typically less than 5.0 ng/mL.

Recombinant Human EPO Protein (rp145653) - SDS-PAGE
2.5 μg/lane of Recombinant Human EPO was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a band at 61.8 kDa.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到6个结果

批号(Lot Number) 证书类型 货号
ZJ23F1001349 分析证书 rp145653
ZJ23F1001350 分析证书 rp145653
ZJ23F1001348 分析证书 rp145653
ZJ25F0319316 分析证书 rp145653
ZJ25F0319303 分析证书 rp145653
ZJ25F0319302 分析证书 rp145653

可替换产品

  1. Recombinant Human Erythropoietin/EPO Protein
    Recombinant Human Erythropoietin/EPO Protein

    ¥639.92

引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al..  (2004)  The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)..  Genome Res,  14  (10B): (2121-7).  [PMID:15489334]
2. Syed RS, Reid SW, Li C, Cheetham JC, Aoki KH, Liu B, Zhan H, Osslund TD, Chirino AJ, Zhang J et al..  (1998)  Efficiency of signalling through cytokine receptors depends critically on receptor orientation..  Nature,  395  (6701): (511-6).  [PMID:9774108]
3. Takeuchi, M M and Kobata, A A..  (1991)  Structures and functional roles of the sugar chains of human erythropoietins..  Glycobiology,  [PMID:1820196]
4. Sasaki, H H, Ochi, N N, Dell, A A and Fukuda, M M..  (1988)  Site-specific glycosylation of human recombinant erythropoietin: analysis of glycopeptides or peptides at each glycosylation site by fast atom bombardment mass spectrometry..  Biochemistry,  (15): [PMID:3219367]
5. Takeuchi, M M and 6 more authors..  (1988)  Comparative study of the asparagine-linked sugar chains of human erythropoietins purified from urine and the culture medium of recombinant Chinese hamster ovary cells..  The Journal of biological chemistry,  (15): [PMID:3346214]
6. Jacobs, K K and 9 more authors..  (1985)  Isolation and characterization of genomic and cDNA clones of human erythropoietin..  Nature,  [PMID:3838366]
7. Lin, F K FK and 9 more authors..  (1985)  Cloning and expression of the human erythropoietin gene..  Proceedings of the National Academy of Sciences of the United States of America,  [PMID:3865178]
8. Lai, P H PH, Everett, R R, Wang, F F FF, Arakawa, T T and Goldwasser, E E..  (1986)  Structural characterization of human erythropoietin..  The Journal of biological chemistry,  (5): [PMID:3949763]
9. Yanagawa, S S and 6 more authors..  (1984)  Isolation of human erythropoietin with monoclonal antibodies..  The Journal of biological chemistry,  (10): [PMID:6698989]
10. Funakoshi, A A, Muta, H H, Baba, T T and Shimizu, S S..  (1993)  Gene expression of mutant erythropoietin in hepatocellular carcinoma..  Biochemical and biophysical research communications,  (15): [PMID:8396923]
11. Glöckner, G G and 6 more authors..  (1998)  Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes..  Genome research,  [PMID:9799793]
12. Hillier, Ladeana W LW and 106 more authors..  (2003)  The DNA sequence of human chromosome 7..  Nature,  (10): [PMID:12853948]
13. Tong, Zongzhong Z and 36 more authors..  (2008)  Promoter polymorphism of the erythropoietin gene in severe diabetic eye and kidney complications..  Proceedings of the National Academy of Sciences of the United States of America,  (13): [PMID:18458324]
14. Camps, Carme C and 10 more authors..  (2016)  Gene panel sequencing improves the diagnostic work-up of patients with idiopathic erythrocytosis and identifies new mutations..  Haematologica,  [PMID:27651169]
15. Kim, Ah Ram AR and 20 more authors..  (2017)  Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation..  Cell,  (9): [PMID:28283061]
16. Zmajkovic, Jakub J and 6 more authors..  (2018)  A Gain-of-Function Mutation in EPO in Familial Erythrocytosis..  The New England journal of medicine,  (8): [PMID:29514032]

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