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Recombinant Human Epigen Protein

  • 表达系统: E. coli
  • Accession #: Q6UW88
  • 蛋白标签: No tag
  • 生物活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/ml, corresponding to a specific activity of > 3.3 × 103 IU/mg.
  • 内毒素水平: <1.0 EU/μg
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库存信息

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库存信息

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库存信息

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货号 (SKU) 包装规格 是否现货 价格 数量
rp145645-10μg
10μg 现货 Stock Image
rp145645-50μg
50μg 现货 Stock Image
rp145645-100μg
100μg 现货 Stock Image
rp145645-1mg
1mg 期货 Stock Image

基本描述

产品名称 Recombinant Human Epigen Protein
别名 重组人类Epigen蛋白
英文别名 EPG | Epithelial mitogen
规格或纯度 ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥98%(SDS-PAGE&HPLC)
产品介绍


Purity

>95% SDS-PAGE. Greater than 98% by SDS-PAGE and HPLC analyses.Endotoxin level is less than 0.1 ng per µg (1EU/µg).


Function

Promotes the growth of epithelial cells. May stimulate the phosphorylation of EGFR and mitogen-activated protein kinases.

生物活性 Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/ml, corresponding to a specific activity of > 3.3 × 103 IU/mg.
内毒素水平 <1.0 EU/μg
表达系统 E. coli
种属 人(Human)
氨基酸 24-95aa
序列 AVTVTPPITAQQADNIEGPIALKFSHLCLEDHNSYCINGACAFHHELEKAICRCFTGYTGERCEHLTLTSYA
纯度 >98% (SDS-PAGE and HPLC)
蛋白标签 No tag
蛋白长度 Protein fragment
无载体 Yes
无动物源 Yes
Accession # Q6UW88
来源 重组表达
预测分子量 7.9 kDa
SDS-PAGE 7.9 kDa

储存与运输

物理形态 冻干(Lyophilized)
储存缓冲液 Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4
复溶 Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL.
储存温度 -20°C储存
运输条件 超低温冰袋运输
稳定性与储存 使用手动解冻冰箱,避免反复冻融循环。自收到之日起 12 个月内,-20 至 -70 °C(按提供时的温度)。1 个月内,复溶后在无菌条件下 2 至 8 °C。3 个月内,复溶后在无菌条件下 -20 至 -70 °C。
分子类型 蛋白质

图片

Recombinant Human Epigen Protein (rp145645)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 300 ng/mL, corresponding to a specific activity of > 3.3 × 10³ IU/mg.

Recombinant Human Epigen Protein (rp145645)-SDS-PAGE
Recombinant Human Epigen Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a single band at 7.9 kDa.

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ23F0600340 分析证书 rp145645
ZJ23F0600338 分析证书 rp145645
ZJ23F0600339 分析证书 rp145645

引用文献

1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al..  (2003)  The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment..  Genome Res,  13  (10): (2265-70).  [PMID:12975309]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al..  (2004)  Complete sequencing and characterization of 21,243 full-length human cDNAs..  Nat Genet,  36  (1): (40-5).  [PMID:14702039]
3. Zhang, Zemin Z and Henzel, William J WJ..  (2004)  Signal peptide prediction based on analysis of experimentally verified cleavage sites..  Protein science : a publication of the Protein Society,  [PMID:15340161]
4. Kochupurakkal, Bose S BS and 15 more authors..  (2005)  Epigen, the last ligand of ErbB receptors, reveals intricate relationships between affinity and mitogenicity..  The Journal of biological chemistry,  (4): [PMID:15611079]

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