Recombinant Human CCL11/Eotaxin Protein

COA

级别和纯度:

≥97%(SDS-PAGE&HPLC)

表达系统: E. coli
Accession #: P51671
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp145581-10μg	10μg	现货
rp145581-20μg	20μg	期货
rp145581-50μg	50μg	现货
rp145581-100μg	100μg	现货
rp145581-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CCL11

Accession#:P51671 (1) CCL11 (2) Gene ID:6356 (2)

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基本描述

产品名称	Recombinant Human CCL11/Eotaxin Protein
别名	重组人嗜酸粒细胞趋化因子 \| 重组人 CCL11/Eotaxin 蛋白
英文别名	C-C motif chemokine 11 \| CCL11 \| chemokine (C-C motif) ligand 11 \| Eosinophil chemotactic protein \| Eotaxin \| eotaxin-1 \| MGC22554 \| SCYA11Small-inducible cytokine A11 \| small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin) \| SCYA11 \| C-C mo
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥97%(SDS-PAGE&HPLC)
产品介绍	Purity > 97 % by SDS-PAGE and HPLC analyses.Endotoxin level is <1 EU/μg Function Human CCL11 is belonging to the CC chemokine family. It is encoded by the gene CCL11. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. CCR3 has been identified to be a specific CCL11 receptor.In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions.
生物活性	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	24-97 aa
序列	GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP
纯度	＞97%(SDS-PAGE, HPLC)
蛋白标签	No tag
蛋白长度	Full length protein
无载体	Yes
无动物源	Yes
Accession #	P51671
来源	重组表达
预测分子量	8.4 kDa

储存与运输

应用	Active, SDS-PAGE
物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 ℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	使用手动除霜冰柜，避免反复冻融循环。自收到之日起 12 个月，-20 至 -70 ℃。1 个月，复溶后无菌条件下 2 至 8 ℃。3 个月，复溶后无菌条件下 -20 至 -70 ℃。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human CCL11/Eotaxin Protein (rp145581)

Recombinant Human CCL11/Eotaxin Protein (rp145581)-Protein Bioactivity
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/mL.

SDS-PAGE: Recombinant Human CCL11/Eotaxin Protein (rp145581)

Recombinant Human CCL11/Eotaxin Protein (rp145581)-SDS-PAGE
Recombinant Human CCL11/Eotaxin Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 9.2 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F0600343	分析证书	rp145581
ZJ23F0600341	分析证书	rp145581
ZJ23F0600342	分析证书	rp145581

技术文档和文章

引用文献

1. Kitaura M, Nakajima T, Imai T, Harada S, Combadiere C, Tiffany HL, Murphy PM, Yoshie O. (1996) Molecular cloning of human eotaxin, an eosinophil-selective CC chemokine, and identification of a specific eosinophil eotaxin receptor, CC chemokine receptor 3.. J Biol Chem, 271 (13): (7725-30). [PMID:8631813]
2. Garcia-Zepeda, E A EA and 5 more authors.. (1996) Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia.. Nature medicine, [PMID:8597956]
3. Ponath, P D PD and 11 more authors.. (1996) Cloning of the human eosinophil chemoattractant, eotaxin. Expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils.. The Journal of clinical investigation, (1): [PMID:8609214]
4. Bartels, J J and 6 more authors.. (1996) Human dermal fibroblasts express eotaxin: molecular cloning, mRNA expression, and identification of eotaxin sequence variants.. Biochemical and biophysical research communications, (23): [PMID:8780731]
5. Garcia-Zepeda, E A EA and 5 more authors.. (1997) Genomic organization, complete sequence, and chromosomal location of the gene for human eotaxin (SCYA11), an eosinophil-specific CC chemokine.. Genomics, (1): [PMID:9169149]
6. Hein, H H and 5 more authors.. (1997) Genomic organization, sequence, and transcriptional regulation of the human eotaxin gene.. Biochemical and biophysical research communications, (28): [PMID:9299399]
7. Noso, N N and 5 more authors.. (1998) Delayed production of biologically active O-glycosylated forms of human eotaxin by tumor-necrosis-factor-alpha-stimulated dermal fibroblasts.. European journal of biochemistry, (1): [PMID:9578468]
8. Crump, M P MP, Rajarathnam, K K, Kim, K S KS, Clark-Lewis, I I and Sykes, B D BD.. (1998) Solution structure of eotaxin, a chemokine that selectively recruits eosinophils in allergic inflammation.. The Journal of biological chemistry, (28): [PMID:9712872]

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