Recombinant Human DLL1 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: O00548
蛋白标签: C-His
生物活性: 1. Measured by its ability to bind human NOTCH1 in a functional ELISA. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL.
内毒素水平: <1.0 EU/μg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp145162-10μg	10μg	现货
rp145162-50μg	50μg	现货
rp145162-100μg	100μg	现货
rp145162-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: DLL1

Accession#:O00548 (1) Dll1 (4) Gene ID:28514 (2)

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基本描述

产品名称	Recombinant Human DLL1 Protein
别名	重组人δ样蛋白1 \| 重组人 DLL1 蛋白
英文别名	delta (Drosophila)-like 1 \| Delta 1 \| Delta \| Delta1 \| delta-like 1 (Drosophila) \| delta-like protein 1 \| DL1 \| DLL1 \| Drosophila Delta homolog 1 \| H-Delta-1 \| NEDBAS \| Delta1 \| Drosophila Delta homolog 1 \| H-Delta-1
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity >95% (SDS-PAGE) Endotoxin level <1.0 EU/μg Function Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. Post-translational Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation.
生物活性	1. Measured by its ability to bind human NOTCH1 in a functional ELISA. 2. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 μg/mL.
内毒素水平	<1.0 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	22-540 aa
序列	SGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPYCTEPICLPGCDEQHGFCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCKNGATCTNTGQGSYTCSCRPGYTGATCELGIDECDPSPCKNGGSCTDLENSYSCTCPPGFYGKICELSAMTCADGPCFNGGRCSDSPDGGYSCRCPVGYSGFNCEKKIDYCSSSPCSNGAKCVDLGDAYLCRCQAGFSGRHCDDNVDDCASSPCANGGTCRDGVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHERGHRYVCECARGYGGPNCQFLLPELPPGPAVVDLTEKLEGQGAHHHHHHHHHH
纯度	>95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	O00548
来源	重组表达
预测分子量	57.4 kDa
SDS-PAGE	58 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from sterile 20mM Tris, 150mM NaCl, 5% Glycerol, 5% Trehalose, 5% Mannitol, 0.01% Tween-80, pH8.0 .
复溶	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20℃ 至 -80℃ 的无菌条件下保存。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human DLL1 protein (rp145162)

Recombinant Human DLL1 protein (rp145162) - Protein Bioactivity
Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 2 - 20 μg/mL.

Protein Bioactivity: Recombinant Human DLL1 protein (rp145162)

Recombinant Human DLL1 protein (rp145162) - Protein Bioactivity
Measured by its ability to bind human NOTCH1 in a functional ELISA. Immobilized Recombinant Human DLL1 protein (rp145162) at 2 μg/mL can bind human NOTCH1.

SDS-PAGE: Recombinant Human DLL1 protein (rp145162)

Recombinant Human DLL1 protein (rp145162) - SDS-PAGE
3 μg/lane of Recombinant Human DLL1 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 61.1 kDa.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102356	分析证书	rp145162
ZJ24F0102357	分析证书	rp145162
ZJ24F0102358	分析证书	rp145162

技术文档和文章

引用文献

1. Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C et al.. (2003) The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.. Genome Res, 13 (10): (2265-70). [PMID:12975309]
2. Gray, G E GE and 9 more authors.. (1999) Human ligands of the Notch receptor.. The American journal of pathology, [PMID:10079256]
3. Han, W W, Ye, Q Q and Moore, M A MA.. (2000) A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells.. Blood, (1): [PMID:10688816]
4. Shimizu, K K, Chiba, S S, Saito, T T, Kumano, K K and Hirai, H H.. (2000) Physical interaction of Delta1, Jagged1, and Jagged2 with Notch1 and Notch3 receptors.. Biochemical and biophysical research communications, (16): [PMID:11006133]
5. Jaleco, A C AC and 7 more authors.. (2001) Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation.. The Journal of experimental medicine, (1): [PMID:11581320]
6. Wright, Gavin J GJ, Leslie, Jonathan D JD, Ariza-McNaughton, Linda L and Lewis, Julian J.. (2004) Delta proteins and MAGI proteins: an interaction of Notch ligands with intracellular scaffolding molecules and its significance for zebrafish development.. Development (Cambridge, England), [PMID:15509766]
7. Wang, Pingzhang P, Yu, Peng P, Gao, Peng P, Shi, Taiping T and Ma, Dalong D.. (2009) Discovery of novel human transcript variants by analysis of intronic single-block EST with polyadenylation site.. BMC genomics, (12): [PMID:19906316]
8. Adam, M Gordian MG and 10 more authors.. (2013) Synaptojanin-2 binding protein stabilizes the Notch ligands DLL1 and DLL4 and inhibits sprouting angiogenesis.. Circulation research, (8): [PMID:24025447]
9. Suzuki, Ikuo K IK and 11 more authors.. (2018) Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation.. Cell, (31): [PMID:29856955]
10. Fischer-Zirnsak, Björn B and 37 more authors.. (2019) Haploinsufficiency of the Notch Ligand DLL1 Causes Variable Neurodevelopmental Disorders.. American journal of human genetics, (19): [PMID:31353024]

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