Recombinant Human CYR61/CCN1 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: E. coli
Accession #: O00622
蛋白标签: No tag
生物活性: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of > 330 IU/mg.
内毒素水平: <1.0 EU/μg

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货号 (SKU)	包装规格	是否现货
rp144926-10μg	10μg	现货
rp144926-50μg	50μg	现货
rp144926-100μg	100μg	现货
rp144926-1mg	1mg	期货
rp144926-250μg	250μg	现货

查看相关系列

点击查看蛋白信息: CYR61

Accession#:O00622 (3) CCN1 (3) CYR61 (3) Gene ID:3491 (3)

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基本描述

产品名称	Recombinant Human CYR61/CCN1 Protein
别名	重组人CYR61/CCN1蛋白 \| 重组人 CYR61/CCN1 蛋白
英文别名	CCN family member 1 \| CCN1 \| CCN1IGF-binding protein 10 \| Cyr61 \| Cysteine-rich angiogenic inducer 61 \| cysteine-rich, angiogenic inducer, 61 \| GIG1 \| GIG1cysteine-rich heparin-binding protein 61 \| IBP-10 \| IGFBP-10 \| IGFBP10cysteine-rich, anigogenic indu
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Purity > 95 % by SDS-PAGE and HPLC analyses. Function Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3. Banckground: Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, when down-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16).
生物活性	Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/ml, corresponding to a specific activity of > 330 IU/mg.
内毒素水平	<1.0 EU/μg
表达系统	E. coli
种属	人(Human)
氨基酸	25-381 aa
序列	TCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFNDIHKFRD
纯度	＞95%(SDS-PAGE, HPLC)
蛋白标签	No tag
蛋白长度	Protein fragment
无载体	Yes
无动物源	Yes
Accession #	O00622
来源	重组表达
预测分子量	39.4 kDa
SDS-PAGE	39.4 kDa

储存与运输

应用	Cell Culture,Active
物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from a 0.2 µm filtered concentrated solution in citrate buffer solution, 300 mM NaCl, pH 3.0
复溶	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ ﹣20℃. Further dilutions should be made in appropriate buffered solutions.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	使用手动除霜冰柜，避免反复冻融循环。自收到之日起 12 个月，-20 至 -70 °C。复溶后 1 个月，无菌条件下 2 至 8 °C。3 个月，复溶后在无菌条件下 -20 至 -70 °C。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human CYR61/CCN1 Protein (rp144926)

Recombinant Human CYR61/CCN1 Protein (rp144926)-Protein Bioactivity
Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine Balb/3T3 cells is less than 3.0 μg/mL, corresponding to a specific activity of > 330 IU/mg.

SDS-PAGE: Recombinant Human CYR61/CCN1 Protein (rp144926)

Recombinant Human CYR61/CCN1 Protein (rp144926)-SDS-PAGE
Recombinant Human CYR61/CCN1 Protein was resolved with SDS-PAGE under reducing (R) and visualized by Coomassie® Blue staining, showing a single band at 39.4 kDa.

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批号(Lot Number)	证书类型	货号
ZJ23F0600503	分析证书	rp144926
ZJ23F0600505	分析证书	rp144926
ZJ23F0600504	分析证书	rp144926
ZJ23F0600506	分析证书	rp144926

技术文档和文章

引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
2. Jay, P P and 5 more authors.. (1997) The human growth factor-inducible immediate early gene, CYR61, maps to chromosome 1p.. Oncogene, (10): [PMID:9135077]
3. Kireeva, M L ML, Lam, S C SC and Lau, L F LF.. (1998) Adhesion of human umbilical vein endothelial cells to the immediate-early gene product Cyr61 is mediated through integrin alphavbeta3.. The Journal of biological chemistry, (30): [PMID:9446626]
4. Martinerie, C C, Viegas-Pequignot, E E, Nguyen, V C VC and Perbal, B B.. (1997) Chromosomal mapping and expression of the human cyr61 gene in tumour cells from the nervous system.. Molecular pathology : MP, [PMID:9536281]
5. Jedsadayanmata, A A, Chen, C C CC, Kireeva, M L ML, Lau, L F LF and Lam, S C SC.. (1999) Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin alpha(IIb)beta(3).. The Journal of biological chemistry, (20): [PMID:10446209]
6. Chen, N N, Chen, C C CC and Lau, L F LF.. (2000) Adhesion of human skin fibroblasts to Cyr61 is mediated through integrin alpha 6beta 1 and cell surface heparan sulfate proteoglycans.. The Journal of biological chemistry, (11): [PMID:10821835]
7. Albrecht, C C and 5 more authors.. (2000) Muscarinic acetylcholine receptors induce the expression of the immediate early growth regulatory gene CYR61.. The Journal of biological chemistry, (15): [PMID:10852911]
8. Grzeszkiewicz, T M TM, Kirschling, D J DJ, Chen, N N and Lau, L F LF.. (2001) CYR61 stimulates human skin fibroblast migration through Integrin alpha vbeta 5 and enhances mitogenesis through integrin alpha vbeta 3, independent of its carboxyl-terminal domain.. The Journal of biological chemistry, (15): [PMID:11287419]
9. Chen, C C CC, Mo, F E FE and Lau, L F LF.. (2001) The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts.. The Journal of biological chemistry, (14): [PMID:11584015]
10. Leng, Esther E, Malcolm, Tom T, Tai, Georgia G, Estable, Mario M and Sadowski, Ivan I.. Organization and expression of the Cyr61 gene in normal human fibroblasts.. Journal of biomedical science, [PMID:11810026]
11. Tagliabracci, Vincent S VS and 14 more authors.. (2015) A Single Kinase Generates the Majority of the Secreted Phosphoproteome.. Cell, (18): [PMID:26091039]

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