Recombinant Human Clusterin Protein

COA

级别和纯度:

≥90%(SDS-PAGE)

表达系统: HEK293
Accession #: P10909-1
蛋白标签: C-His
生物活性: Measured by its ability to induce clustering of Caki‑2 human clear cell carcinoma epithelial cells.
内毒素水平: <0.1 EU/μg

有货

库存信息

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货号 (SKU)	包装规格	是否现货
rp144473-10μg	10μg	现货
rp144473-50μg	50μg	现货
rp144473-100μg	100μg	现货
rp144473-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CLU

Accession#:P10909 (3) CLU (3) Clusterin (3) Gene ID:1191 (3)

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基本描述

产品名称	Recombinant Human Clusterin Protein
别名	重组人Clusterin alpha chain蛋白 \| 重组人类丛集蛋白
英文别名	40 \| AAG4 \| Aging-associated gene 4 protein \| aging-associated protein 4 \| Apo-J \| APOJ \| ApoJalpha \| ApoJbeta \| Apolipoprotein J \| CLI \| CLU \| CLUS_HUMAN \| Clusterin \| Clusterin alpha chain \| Complement cytolysis inhibitor \| Complement cytolysis inhibito
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥90%(SDS-PAGE)
产品介绍	Purity >90% SDS-PAGE. Function Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Post-translational Isoform 1 is proteolytically cleaved on its way through the secretory system, probably within the Golgi lumen. Polyubiquitinated, leading to proteasomal degradation. Heavily N-glycosylated. About 30% of the protein mass is comprised of complex N-linked carbohydrate.
生物活性	Measured by its ability to induce clustering of Caki‑2 human clear cell carcinoma epithelial cells.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	23-449 aa
序列	DQTVSDNELQEMSNQGSKYVNKEIQNAVNGVKQIKTLIEKTNEERKTLLSNLEEAKKKKEDALNETRESETKLKELPGVCNETMMALWEECKPCLKQTCMKFYARVCRSGSGLVGRQLEEFLNQSSPFYFWMNGDRIDSLLENDRQQTHMLDVMQDHFSRASSIIDELFQDRFFTREPQDTYHYLPFSLPHRRPHFFFPKSRIVRSLMPFSPYEPLNFHAMFQPFLEMIHEAQQAMDIHFHSPAFQHPPTEFIREGDDDRTVCREIRHNSTGCLRMKDQCDKCREILSVDCSTNNPSQAKLRRELDESLQVAERLTRKYNELLKSYQWKMLNTSSLLEQLNEQFNWVSRLANLTQGEDQYYLRVTTVASHTSDSDVPSGVTEVVVKLFDSDPITVTVPVEVSRKNPKFMETVAEKALQEYRKKHREEHHHHHH
纯度	＞90%(SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P10909-1
来源	重组表达
预测分子量	47 kDa
SDS-PAGE	36.2 kDa, under reducing conditions; 60.7 kDa, under non-reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from PBS, pH 7.4, 5% Trehalose, 5% Mannitol
复溶	Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human Clusterin Protein (rp144473)

Recombinant Human Clusterin Protein (rp144473) - Protein Bioactivity
Measured by its ability to induce clustering of Caki‑2 human clear cell carcinoma epithelial cells. The effective concentration is 10μg/mL.

SDS-PAGE: Recombinant Human Clusterin Protein (rp144473)

Recombinant Human Clusterin Protein (rp144473) - SDS-PAGE
3 μg/lane of Recombinant Human Clusterin Protein (rp144473) was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 36.2 kDa under reducing conditions and 60.7 kDa under non-reducing conditions.

ELISA: Recombinant Human Clusterin Protein (rp144473)

Recombinant Human Clusterin Protein (rp144473) - ELISA
Immobilized Recombinant Human Clusterin Protein (rp144473) at 1.0 μg/mL can bind AB-16B5 (anti-Clusterin) (Ab177846) with the EC50 of 33.58 ng/mL.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0102327	分析证书	rp144473
ZJ24F0102328	分析证书	rp144473
ZJ24F0102329	分析证书	rp144473

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