Recombinant Human DNAM-1/CD226 Protein

COA

级别和纯度:

≥95%(SDS-PAGE)

表达系统: HEK293
Accession #: Q15762
蛋白标签: C-hFc & His
生物活性: Immobilized Recombinant Human CD226 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD155 with a linear range of 0.3-1.2 μg/mL.
内毒素水平: <0.1 EU/μg

有货

库存信息

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库存信息

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库存信息

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库存信息

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货号 (SKU)	包装规格	是否现货
rp143887-10μg	10μg	现货
rp143887-50μg	50μg	现货
rp143887-100μg	100μg	现货
rp143887-1mg	1mg	期货

查看相关系列

点击查看蛋白信息: CD226

Accession#:Q15762 (2) CD226 (7) DNAM-1 (11) Gene ID:10666 (2)

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基本描述

产品名称	Recombinant Human DNAM-1/CD226 Protein
别名	CD226分子(CD226)重组蛋白 \| 重组人类 DNAM-1/CD226 蛋白
英文别名	DNAM-1 \| DNAX accessory molecule 1 \| adhesion glycoprotein \| CD226 \| CD226 antigen \| CD226 molecule \| CD226_HUMAN \| DNAM 1 \| DNAM-1 \| DNAM1 \| DNAX accessory molecule 1 \| platelet and T cell activation antigen 1 \| PTA1 \| T lineage specific activation antig
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% (SDS-PAGE) Endotoxin level ＜0.1 EU/μg Function Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell.
生物活性	Immobilized Recombinant Human CD226 at 2 μg/mL (100 μL/well) can bind Recombinant Human CD155 with a linear range of 0.3-1.2 μg/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	19-247 aa
序列	EEVLWHTSVPFAENMSLECVYPSMGILTQVEWFKIGTQQDSIAIFSPTHGMVIRKPYAERVYFLNSTMASNNMTLFFRNASEDDVGYYSCSLYTYPQGTWQKVIQVVQSDSFEAAVPSNSHIVSEPGKNVTLTCQPQMTWPVQAVRWEKIQPRQIDLLTYCNLVHGRNFTSKFPRQIVSNCSHGRWSVIVIPDVTVSDSGLYRCYLQASAGENETFVMRLTVAEGKTDNENLYFQGMDPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-hFc & His
无载体	Yes
无动物源	Yes
Accession #	Q15762
来源	重组表达
预测分子量	54 kDa
SDS-PAGE	80-90 kDa, under reducing conditions

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	在 -20~-80℃ 下可保存 1 年以上。收货后建议分装。避免冷冻/解冻循环。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human DNAM-1/CD226 Protein (rp143887)

Recombinant Human DNAM-1/CD226 Protein (rp143887) - Protein Bioactivity
Immobilized Recombinant Human DNAM-1/CD226 Protein (rp143887) at 4.0 μg/mL can bind Biotinylated Human CD155 with a linear range of 0.02 - 0.625 μg/mL. The ED ₅₀ for this effect is 0.90 μg/mL.

SDS-PAGE: Recombinant Human DNAM-1/CD226 Protein (rp143887)

Recombinant Human DNAM-1/CD226 Protein (rp143887) - SDS-PAGE
3 μg/lane of Recombinant Human DNAM-1/CD226 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 80.0 kDa under reducing conditions and 80.0 & 160.0 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ24F0606788	分析证书	rp143887
ZJ24F0606786	分析证书	rp143887
ZJ24F0606787	分析证书	rp143887

技术文档和文章

引用文献

1. Zhu Y, Paniccia A, Schulick AC, Chen W, Koenig MR, Byers JT, Yao S, Bevers S, Edil BH. (2016) Identification of CD112R as a novel checkpoint for human T cells.. J Exp Med, 213 (2): (167-76). [PMID:26755705]
2. Shibuya, A A and 10 more authors.. (1996) DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes.. Immunity, [PMID:8673704]
3. Tahara-Hanaoka, Satoko S and 8 more authors.. (2004) Functional characterization of DNAM-1 (CD226) interaction with its ligands PVR (CD155) and nectin-2 (PRR-2/CD112).. International immunology, [PMID:15039383]
4. Pende, Daniela D and 13 more authors.. (2005) PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis.. Molecular immunology, [PMID:15607800]
5. Nusbaum, Chad C and 54 more authors.. (2005) DNA sequence and analysis of human chromosome 18.. Nature, (22): [PMID:16177791]
6. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087]

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