货号 (SKU)

包装规格

是否现货

价格

数量

rp143784-10μg

10μg

现货

rp143784-20μg

20μg

现货

rp143784-100μg

100μg

现货

rp143784-1mg

1mg

期货

基本描述

产品名称	Recombinant Human gp130 Protein
别名	重组人糖蛋白130 \| 重组人 CD130 (gp130) 蛋白
英文别名	CD130 antigen \| CD130 \| CDw130 \| DKFZp564F053 \| gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form \| gp130 \| IL-6 receptor subunit beta \| IL-6R subunit beta \| IL-6RB \| IL-6R-beta \| IL6ST \| interleukin 6 signal transducer (gp130, onco
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, High performance, ≥95%(SDS-PAGE)
产品介绍	Function Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. Post-translational Phosphorylation of Ser-782 down-regulates cell surface expression. Heavily N-glycosylated.
生物活性	Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha.The ED₅₀ for this effect is 0.65-6.5 ng/mL.
内毒素水平	<0.01 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	23-618 aa
序列	ELLDPCGYISPESPVVQLHSNFTAVCVLKEKCMDYFHVNANYIVWKTNHFTIPKEQYTIINRTASSVTFTDIASLNIQLTCNILTFGQLEQNVYGITIISGLPPEKPKNLSCIVNEGKKMRCEWDGGRETHLETNFTLKSEWATHKFADCKAKRDTPTSCTVDYSTVYFVNIEVWVEAENALGKVTSDHINFDPVYKVKPNPPHNLSVINSEELSSILKLTWTNPSIKSVIILKYNIQYRTKDASTWSQIPPEDTASTRSSFTVQDLKPFTEYVFRIRCMKEDGKGYWSDWSEEASGITYEDRPSKAPSFWYKIDPSHTQGYRTVQLVWKTLPPFEANGKILDYEVTLTRWKSHLQNYTVNATKLTVNLTNDRYLATLTVRNLVGKSDAAVLTIPACDFQATHPVMDLKAFPKDNMLWVEWTTPRESVKKYILEWCVLSDKAPCITDWQQEDGTVHRTYLRGNLAESKCYLITVTPVYADGPGSPESIKAYLKQAPPSKGPTVRTKKVGKNEAVLEWDQLPVDVQNGFIRNYTIFYRTIIGNETAVNVDSSHTEYTLSSLTSDTLYMVRMAAYTDEGGKDGPEFTFTTPKFAQGEI
纯度	＞95%(SDS-PAGE)
蛋白标签	C-hFc & His
N-末端序列	Glu
无载体	Yes
无动物源	Yes
Accession #	P40189
来源	重组表达
预测分子量	95 kDa

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	PBS, containing 5% trehalose, 5% mannitol
复溶	Reconstitute in water to a concentration of 0.1-1.0 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	自收到之日起 12 个月，-20 至 -70 ℃。 1 个月，复溶后无菌条件下 2 至 8 ℃。 3 个月，复溶后无菌条件下 -20 至 -70 ℃。
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human gp130 Protein (rp143784)

Recombinant Human gp130 Protein (rp143784) - Protein Bioactivity
Measured by its ability to inhibit IL-6-dependent proliferation of the 7TD1 mouse hybridoma cells in the presence of Recombinant Human IL-6 R alpha. The ED₅₀ for this effect is 2.4 ng/mL.

SDS-PAGE: Recombinant Human gp130 Protein (rp143784)

Recombinant Human gp130 Protein (rp143784) - SDS-PAGE
4 μg/lane of Recombinant Human gp130 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 122.7 kDa under reducing conditions and 250.0 kDa under non-reducing conditions.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ23F1201896	分析证书	rp143784
ZJ23F1201895	分析证书	rp143784
ZJ23F1201894	分析证书	rp143784

技术文档和文章

阿拉丁®靶点蛋白

Categories: 技术文章

探索主题:

药物开发信号通路细胞因子靶点蛋白药物靶点受体免疫检查点细胞表面标记物

可替换产品

引用文献

1. Boulanger MJ, Chow DC, Brevnova EE, Garcia KC. (2003) Hexameric structure and assembly of the interleukin-6/IL-6 alpha-receptor/gp130 complex.. Science, 300 (5628): (2101-4). [PMID:12829785]
2. Bravo J, Staunton D, Heath JK, Jones EY. (1998) Crystal structure of a cytokine-binding region of gp130.. EMBO J, 17 (6): (1665-74). [PMID:9501088]
3. Wilde MI, Faulds D. (1998) Oprelvekin: a review of its pharmacology and therapeutic potential in chemotherapy-induced thrombocytopenia.. BioDrugs, 10 (2): (159-71). [PMID:18020592]
4. Berl T, Schwertschlag U. (2000) Preclinical pharmacologic basis for clinical use of rhIL-11 as an effective platelet-support agent.. Oncology (Williston Park, NY), 14 (9 Suppl 8): (12-20). [PMID:11033834]
5. Van Roy M, Ververken C, Beirnaert E, Hoefman S, Kolkman J, Vierboom M, Breedveld E, 't Hart B, Poelmans S, Bontinck L et al.. (2015) The preclinical pharmacology of the high affinity anti-IL-6R Nanobody® ALX-0061 supports its clinical development in rheumatoid arthritis.. Arthritis Res Ther, 17 (135). [PMID:25994180]
6. Anna Wonnerth,Katharina M Katsaros,Konstantin A Krychtiuk,Walter S Speidl,Christoph Kaun,Kylie Thaler,Kurt Huber,Johann Wojta,Gerald Maurer,Ingebjorg Seljeflot,Harald Arnesen,Thomas W Weiss. (2014-03-19) Glycoprotein 130 polymorphism predicts soluble glycoprotein 130 levels.. Metabolism: clinical and experimental, 63 ((5)): (647-653). [PMID:24629561]
7. Li H, Xiao H, Lin L, Jou D, Kumari V, Lin J, Li C.. (2014) Drug design targeting protein-protein interactions (PPIs) using multiple ligand simultaneous docking (MLSD) and drug repositioning: discovery of raloxifene and bazedoxifene as novel inhibitors of IL-6/GP130 interface.. J Med Chem, 57 (3): (632-641). [PMID:24456369] [10.1021/jm401144z]
8. Song D,Yu W,Ren Y,Zhu J,Wan C,Cai G,Guo J,Zhang W,Kong L. (2020) Discovery of bazedoxifene analogues targeting glycoprotein 130.. Eur J Med Chem, 199 (112375-112375). [PMID:32388278] [10.1016/j.ejmech.2020.112375]
9. Hibi, M M and 5 more authors.. (1990) Molecular cloning and expression of an IL-6 signal transducer, gp130.. Cell, (21): [PMID:2261637]
10. Mosley, B B and 6 more authors.. (1996) Dual oncostatin M (OSM) receptors. Cloning and characterization of an alternative signaling subunit conferring OSM-specific receptor activation.. The Journal of biological chemistry, (20): [PMID:8999038]
11. Gibson, R M RM and 5 more authors.. (2000) Phosphorylation of human gp130 at Ser-782 adjacent to the Di-leucine internalization motif. Effects on expression and signaling.. The Journal of biological chemistry, (21): [PMID:10811661]
12. Tanaka, M M and 7 more authors.. (2000) Cloning of novel soluble gp130 and detection of its neutralizing autoantibodies in rheumatoid arthritis.. The Journal of clinical investigation, [PMID:10880057]
13. Moritz, R L RL, Hall, N E NE, Connolly, L M LM and Simpson, R J RJ.. (2001) Determination of the disulfide structure and N-glycosylation sites of the extracellular domain of the human signal transducer gp130.. The Journal of biological chemistry, (16): [PMID:11098061]
14. Jostock, T T and 8 more authors.. (2001) Soluble gp130 is the natural inhibitor of soluble interleukin-6 receptor transsignaling responses.. European journal of biochemistry, [PMID:11121117]
15. Li, H H, Wang, H H and Nicholas, J J.. (2001) Detection of direct binding of human herpesvirus 8-encoded interleukin-6 (vIL-6) to both gp130 and IL-6 receptor (IL-6R) and identification of amino acid residues of vIL-6 important for IL-6R-dependent and -independent signaling.. Journal of virology, [PMID:11238858]
16. Chow , D D, He , X X, Snow, A L AL, Rose-John, S S and Garcia, K C KC.. (2001) Structure of an extracellular gp130 cytokine receptor signaling complex.. Science (New York, N.Y.), (16): [PMID:11251120]
17. Boulanger, Martin J MJ, Bankovich, Alexander J AJ, Kortemme, Tanja T, Baker, David D and Garcia, K Christopher KC.. (2003) Convergent mechanisms for recognition of divergent cytokines by the shared signaling receptor gp130.. Molecular cell, [PMID:14527405]
18. Schmutz, Jeremy J and 75 more authors.. (2004) The DNA sequence and comparative analysis of human chromosome 5.. Nature, (16): [PMID:15372022]
19. Liu, Tao T and 6 more authors.. Human plasma N-glycoproteome analysis by immunoaffinity subtraction, hydrazide chemistry, and mass spectrometry.. Journal of proteome research, [PMID:16335952]
20. Sjöblom, Tobias T and 28 more authors.. (2006) The consensus coding sequences of human breast and colorectal cancers.. Science (New York, N.Y.), (13): [PMID:16959974]
21. Zahedi, René P RP and 8 more authors.. (2008) Phosphoproteome of resting human platelets.. Journal of proteome research, [PMID:18088087]
22. Hashimoto, Yuichi Y, Kurita, Megumi M, Aiso, Sadakazu S, Nishimoto, Ikuo I and Matsuoka, Masaaki M.. (2009) Humanin inhibits neuronal cell death by interacting with a cytokine receptor complex or complexes involving CNTF receptor alpha/WSX-1/gp130.. Molecular biology of the cell, [PMID:19386761]
23. Waetzig, Georg H GH and 12 more authors.. (2010) N-linked glycosylation is essential for the stability but not the signaling function of the interleukin-6 signal transducer glycoprotein 130.. The Journal of biological chemistry, (15): [PMID:19915009]
24. Xu, Yibin Y and 9 more authors.. (2010) Crystal structure of the entire ectodomain of gp130: insights into the molecular assembly of the tall cytokine receptor complexes.. The Journal of biological chemistry, (9): [PMID:20489211]
25. Schütt, Antje A and 6 more authors.. (2013) gp130 activation is regulated by D2-D3 interdomain connectivity.. The Biochemical journal, (15): [PMID:23294003]
26. Sun, Luguo L and 8 more authors.. Low incidence of IL6ST (gp130) mutations in exon 6 in lung cancer of a Chinese cohort.. Cancer genetics, [PMID:25242236]
27. Taniguchi, Koji K and 17 more authors.. (2015) A gp130-Src-YAP module links inflammation to epithelial regeneration.. Nature, (5): [PMID:25731159]
28. Lokau, Juliane J and 13 more authors.. (2016) Proteolytic Cleavage Governs Interleukin-11 Trans-signaling.. Cell reports, (23): [PMID:26876177]
29. Schwerd, Tobias T and 24 more authors.. (2017) A biallelic mutation in IL6ST encoding the GP130 co-receptor causes immunodeficiency and craniosynostosis.. The Journal of experimental medicine, (4): [PMID:28747427]
30. Shahin, Tala T and 16 more authors.. (2019) Selective loss of function variants in IL6ST cause Hyper-IgE syndrome with distinct impairments of T-cell phenotype and function.. Haematologica, [PMID:30309848]
31. Chen, Yin-Huai and 26 more authors.. (2020) Absence of GP130 cytokine receptor signaling causes extended Stüve-Wiedemann syndrome.. The Journal of experimental medicine, (2): [PMID:31914175]
32. Béziat, Vivien and 59 more authors.. (2020) Dominant-negative mutations in human IL6ST underlie hyper-IgE syndrome.. The Journal of experimental medicine, (1): [PMID:32207811]
33. Materna-Kiryluk, Anna and 17 more authors.. (2021) Mosaic IL6ST variant inducing constitutive GP130 cytokine receptor signaling as a cause of neonatal onset immunodeficiency with autoinflammation and dysmorphy.. Human molecular genetics, (30): [PMID:33517393]

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

Recombinant Human gp130 Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器