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基本描述
| 产品名称 | UltraBio™ Plus PAGE预制胶（Hepes） | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| 产品介绍 |
阿拉丁的UltraBio™ Plus PAGE预制胶是一种使用安全、便捷、高品质的常规尺寸聚丙烯酰胺预制凝胶，仅需40-50分钟即可完成电泳并获得非常平整锐利的条带。本预制胶采用Hepes缓冲系统并有1.5厘米高的浓缩胶，具有非常优良的分离效果，电泳后蛋白条带平整、清晰、细腻、锐利，几乎没有边缘效应；同时本预制胶胶板为玻璃材质，减少了对蛋白的非特异性吸附，电泳效果更好，达到甚至超过了自配PAGE胶的电泳效果，常用于PAGE和Western检测。
本预制胶含有1.5厘米高的4%浓缩胶，可以有效确保获得非常锐利的条带。 本预制胶不能使用常用的Tris-Glycine缓冲系统的SDS-PAGE电泳液，必须使用Hepes缓冲系统的电泳液。
关于10孔和15孔预制胶的选择：需要检测的样品数量多或者需要定量时，推荐使用15孔预制胶，通量更大、更便于进行较多样品的定量统计分析；需要获得非常漂亮的代表性图片时，推荐使用10孔预制胶，10孔预制胶获得的条带更加平整和锐利。 本产品电泳时间短。本预制胶在150V电压下，仅需40-50分钟即可完成电泳并获得非常平整和锐利的电泳条带，而常规的Tris-Glycine电泳系统需要电泳90-120分钟。
注意事项： 本预制胶电泳后可以使用Tris-Glycine缓冲系统的转膜液进行转膜。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。 UltraBio™ Plus Precast PAGE Gel(Hepes) is a high-quality, ready-to-use gel in regular size. With a Hepes electrophoresis buffer, it only takes 40-50 minutes to complete electrophoresis, and very flat and sharp bands can be obtained. This precast gel has a 1.5cm high 4% stacking gel, with a very good protein separation effect. At the same time, this product uses glass plates to reduce non-specific adsorptions. This product has identical or even better performance than home-made PAGE gels and can be routinely used for PAGE and Western analysis. Aladdin provides a variety of UltraBio™ precast gradient/fixed gels of different concentrations with 10 or 15 loading wells. Gradient gels are available in concentrations of 4-15%, 4-20% and 8-20%. Fixed gels include 6%, 8%, 10%, 12% and 15% gels. The gradient gels of 4-20% are most commonly used. Please refer to the table below for the optimal protein separation range for each type of UltraBio™ Plus Precast PAGE Gels (Hepes).
The ratio of acrylamide to bisacrylamide is 29:1, and the gel thickness is 1.5mm. The maximum loading volume per well of 10-well and 15-well gels is 60μl and 30μl, respectively. The plate size is 98×84×4.1mm (width × height × thickness), with a gel size of 81×74×1.5mm (width × height × thickness). Polyacrylamide gel electrophoresis (PAGE) technology is widely used in protein separation, purification, detection, identification, molecular weight determination and other experiments. It is one of the most basic experimental techniques in life science research. Unlike traditional Tris-Glycine gels, UltraBio™ Plus PAGE precast gels for Hepes system are prepared with neutral pH Hepes buffer and do not contain SDS. They can be used for both denaturing and native protein electrophoresis. After electrophoresis, protein can be transferred from the gel to blot membrane with the Tris-Glycine buffer system.Selection of 10-well and 15-well precast gels: Protein bands obtained by 10-well precast gels are flatter and sharper, but when the number of samples to be examined is large or protein quantification is required, we recommend using the 15-well precast gel which has greater throughput and is more convenient for statistical quantitation analysis. This product is safe and convenient to use. There is no need for gel preparation and casting, thus avoiding contact with toxic and irritating reagents. After electrophoresis, the gel can be easily removed from the glass plates. The quality of this product is stable, with high consistency between batches. This product has good protein resolution performance. Proteins in the gel can be transferred efficiently as well. The electrophoresis time is short. The electrophoresis can be completed in 40-50 minutes at a 150V voltage. The run time varies depending on experimental requirements.
Precautions: Do NOT freeze this product at temperatures below 0℃. The electrophoresis buffer in the inner chamber of the electrophoresis tank and the transfer buffer should be freshly prepared. Low-quality buffers, recycled buffers, or buffers that have been kept for too long period of time will result in poor protein resolution and transfer. This product is for R&D only. Not for drug, household, or other uses. For your safety and health, please wear a lab coat and disposable gloves during the operation. |
储存与运输
| 储存温度 | 2-8°C储存 |
|---|---|
| 运输条件 | 冰袋运输 |
| 稳定性与储存 | 室温保存，一年有效。4℃保存，18个月有效。切勿置于0℃以下冷冻。 |
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