2-硝基苯基-β-D-吡喃半乳糖苷(ONPG)

β-半乳糖苷酶底物。

64篇引用文献

COA

级别和纯度:

无动物源

≥99%

CAS编号: 369-07-3
分子式: C₁₂H₁₅O₈N
分子量: 301.25
Beilstein号: 92207
EC号: 206-716-1
MDL号: MFCD00063255
PubChem编号: 96647
PubChem SID: 504756328

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货号 (SKU)	包装规格	是否现货
N109040-1g	1g	现货
N109040-5g	5g	现货
N109040-25g	25g	现货
N109040-100g	100g	现货

查看相关系列

Biochemical Assay Reagents (972) β-半乳糖苷酶底物 (1) 化学生物学 (1256) 半乳糖 (9) 半乳糖苷（酶底物） (47) 单糖 (362) 酶用糖底物 (4)

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基本描述

别名	2-硝基苯基-β-D-吡喃半乳糖苷(ONPG) \| 邻硝基苯-β-D-吡喃半乳糖苷 \| o-硝基苯-β-D-半乳糖苷
英文别名	ONPG \| 2-Nitrophenyl b-D-galactopyranoside \| o-Nitrophenyl beta-D-galactopyranoside \| 2-Nitrophenyl-beta-D-galactopyranoside \| 2-Nitrophenyl beta-D-galactoside \| 2-Nitrophenyl beta-D-galactopyranoside
规格或纯度	无动物源, ≥99%
英文名称	2-Nitrophenyl β-D-galactopyranoside
生化机理	β-半乳糖苷酶可将乳糖分解为半乳糖和葡萄糖。2-Nitrophenyl β-D-galactopyranoside 水解后会释放出半乳糖和一种黄色显色化合物。该检测底物不依赖于诱导型或组成型渗透酶进入细胞，因此反应迅速，可在 24 小时内发生。经 β-半乳糖苷酶水解后生成半乳糖和原硝基苯酚，呈黄色。
应用	用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。
储存温度	-20°C储存,充氩
运输条件	超低温冰袋运输
备注	如果有可能，您尽量在使用的当天配置溶液，并在当天使用完它。但是，如果您需要预先配制储备溶液，我们建议您将溶液等份保存在-20°C的密封小瓶中。通常，它们最多可以使用一个月。在使用前和打开样品瓶之前，我们建议您让您的产品在室温下平衡至少1小时。需要更多关于溶解度，用法和处理的建议吗？请访问我们的常见问题（FAQ）页面以获取更多详细信息。
产品介绍	溶于水、甲醇，水中溶解度可达15mg/ml。有吸湿性。用于检测β-半乳糖苷酶(β-galactosidase)活性，是β-半乳糖苷酶的显色底物。经β-半乳糖苷酶催化后可产生黄色产物，在410－420nm波长可以检测吸光度。 Chromogenic substrate for β-galactosidase 2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.
纯度	≥99%

关联靶点（人）

HepG2 (196354 活性数据)

点击查看此靶标相关信息

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

关联靶点（其它种属）

GCN5 Histone acetyltransferase GCN5 (89 活性数据)

点击查看靶蛋白信息： GCN5

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

NS1 Nonstructural protein 1 (33327 活性数据)

点击查看靶蛋白信息： NS1

活性类型	Relation	Activity value	Units	Action Type	期刊	PubMed Id	doi	Assay Aladdin ID

作用机制

作用机制	Action Type	target ID	Target Name	Target Type	Target Organism	Binding Site Name	参考文献

名称和识别符

PubChem SID	504756328
EC号	206-716-1
分子类型	未知
IIUPAC Name	(2R,3R,4S,5R,6S)-2-(hydroxymethyl)-6-(2-nitrophenoxy)oxane-3,4,5-triol
INCHI	1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChi Key	KUWPCJHYPSUOFW-YBXAARCKSA-N
Smiles	C1=CC=C(C(=C1)[N+](=O)[O-])OC2C(C(C(C(O2)CO)O)O)O
Isomeric SMILES	C1=CC=C(C(=C1)[N+](=O)[O-])O[C@H]2[C@@H]([C@H]([C@H]([C@H](O2)CO)O)O)O
分子量	301.25
Beilstein号	92207
Reaxy-Rn	15247465
Reaxys-RN link address	https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=15247465&ln=

化学和物理性质

溶解性	Soluble in water
密度	1.599
敏感性	对光敏感
比旋光度	-68.8 ° (C=1, H2O)
熔点	195°C
分子量	301.250 g/mol
XLogP3	-1.200
氢键供体数Hydrogen Bond Donor Count	4
氢键受体数Hydrogen Bond Acceptor Count	8
可旋转键计数Rotatable Bond Count	3
精确质量Exact Mass	301.08 Da
单同位素质量Monoisotopic Mass	301.08 Da
拓扑极表面积Topological Polar Surface Area	145.000 Å²
重原子数Heavy Atom Count	21
形式电荷Formal Charge	0
复杂度Complexity	362.000
同位素原子数Isotope Atom Count	0
定义的原子立体中心计数Defined Atom Stereocenter Count	5
未定义的原子立体中心计数Undefined Atom Stereocenter Count	0
定义的键立体中心计数Defined Bond Stereocenter Count	0
未定义的键立体中心计数Undefined Bond Stereocenter Count	0
所有立体化学键的总数The total count of all stereochemical bonds	0
共价键合单元计数Covalently-Bonded Unit Count	1

安全和危险性（GHS）

WGK Germany	3
个人防护装备	Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

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批号(Lot Number)	证书类型	货号
F2527562	分析证书	N109040
F2527563	分析证书	N109040
F2527564	分析证书	N109040
F2527565	分析证书	N109040
B2528492	分析证书	N109040
B2528493	分析证书	N109040
B2528500	分析证书	N109040
H2428393	分析证书	N109040
H2428401	分析证书	N109040
H2428402	分析证书	N109040
H2428403	分析证书	N109040
C2429655	分析证书	N109040
C2429668	分析证书	N109040
G2227414	分析证书	N109040
G2227416	分析证书	N109040
G2227430	分析证书	N109040
G2227431	分析证书	N109040
L2325017	分析证书	N109040
G2408085	分析证书	N109040
A2217074	分析证书	N109040
C1809059	分析证书	N109040
B2223478	分析证书	N109040
B2223479	分析证书	N109040
B2223512	分析证书	N109040
B2223513	分析证书	N109040
K2309063	分析证书	N109040

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