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基本描述
| 产品名称 | 荧光素酶 来源于萤火虫 |
|---|---|
| 英文别名 | Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) | firefly luciferase | luciferase (firefly luciferin) | Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing) | firefly luciferin luciferase | Photinus pyralis luciferase |
| 规格或纯度 | EnzymoPure™, 重组, lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein |
| 产品介绍 |
分子量：mol wt 120 kDa (two subunits) Product introduction Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging. Application The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase. |
储存与运输
| 浓度 | lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein |
|---|---|
| 储存温度 | -80℃储存 |
| 运输条件 | 超低温冰袋运输 |
| CAS编号和信息 | 61970-00-1 |
| 酶学委员会编号 | 1.13.12.7 |
| 单位定义 | One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C. |
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 C2306046 |
分析证书 | L465180 |