L398135	Component	1 KU	5 KU	Storage
L398135A	NruI	50 μL	250 μL	-20°C. Avoid freeze/thaw cycle
L398135B	10×Cut Reaction Buffer	400 μL	2 mL	-20°C. Avoid freeze/thaw cycle

来源

E.coli

酶切位点

5’…TCG↓CGA…3’
3’…AGC↑GCT…5’

酶活定义

在 50 μl 总反应体系中，37℃条件下，1 h内酶切 1 μg λDNA 所需的酶量定义为 1 个活力单位（U）。

产品使用步骤

（1）按照下表所示配置混合溶液

Component	volume
RNase-free Water	To 50 μl
10×Cut Reaction Buffer	5 μl
DNA	1 μg
NruI	1 μl*

(2) 轻柔吸打混匀，切勿振荡涡旋，然后瞬时离心。
(3) 37°C孵育 30 min。
注：*Nrul 最后加入体系中，一般 50 μl 反应体系加入1μl 的Nrul，也可根据实验进行调整，建议酶的加入量不超过总体系的10%，避免产生星活性。

注意事项
(1) Nrul 的甲基化敏感性:
Dam 甲基化:甲基化与酶切位点可能重叠导致切割受损
Dcm 甲基化:不敏感
CpG 甲基化: 阻断切割
(2) 在梅切反应前，尽量确保DNA不含苯酚、氯仿、酒精、EDTA 等杂质，以免影响酶切效果。
(3) 本产品仅作科学研究使用，不得用于其它用途。

Storage buffer: 10mM Tris-HCl, 50mM KCl, 1mM DTT, 0.1mM EDTA, 200µg/ml Recombinant Albumin, 50% Glycerol, pH 7.4

NruI can recognize the TCG/CGA palindromic sequence, and form a flat terminal after enzyme digestion. The isozymes of NRUI include Bsp68I, BtuMI and RruI. Recombinant albumin was added to both NruI and 10×Cut Reaction Buffer to ensure the safety and stability of the product.

Product composition

L398135	Component	1 KU	5 KU	Storage
L398135A	NruI	50 μL	250 μL	-20°C. Avoid freeze/thaw cycle
L398135B	10×Cut Reaction Buffer	400 μL	2 mL	-20°C. Avoid freeze/thaw cycle

Source
E.coli

Restriction Enzyme CuttingSite
5’…TCG↓CGA…3’
3’…AGC↑GCT…5’

Enzyme Activity Definition

In a total reaction system of 50 μl, the amount of enzyme required for enzyme digestion of 1 μg λDNA within 1 h at 37℃ was defined as one active single site (U).
Protocol
(1) Configure the reaction system, as shown in the following table

Component	volume
RNase-free Water	To 50 μl
10×Cut Reaction Buffer	5 μl
DNA	1 μg
NruI	1 μl*

(2) Gently mix, do not oscillate the vortex, and then instantaneous centrifugation.
(3) Incubation at 37°C for 30 min.
Note: *Nrul is finally added to the system, generally 50 μl reaction system to add 1μl of Nrul, can also be adjusted according to the experiment, it is recommended that the amount of enzyme added does not exceed 10% of the total system to avoid star activity.
Cautions
(1) Methylation sensitivity of Nrul:
Dam methylation: Methylation and cleavage sites may overlap resulting in impaired cleavage
Dcm methylation: insensitive
CpG methylation: Blocking cleavage
(2) Before the Meche reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, so as not to affect the effect of enzyme digestion.
(3) This product is for scientific research only and shall not be used for other purposes.

储存与运输

物理形态	液体
浓度	20 U/μl
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20℃ long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
分子类型	酶

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

技术文档和文章

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度

NruI

库存信息

库存信息

库存信息

基本描述

储存与运输

质检证书（CoA,COO,BSE/TSE 和分析图谱)

技术文档和文章

可替换产品

溶液计算器

摩尔浓度计算器

稀释计算器

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