货号 (SKU)

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是否现货

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数量

H293861-10μg

10μg

现货

H293861-50μg

50μg

现货

H293861-100μg

100μg

期货

H293861-1mg

1mg

期货

基本描述

产品名称	Recombinant Human beta 2 Microglobulin Protein
别名	重组人β2微球蛋白
英文别名	B2M \| B2MG_HUMAN \| Beta 2 microglobin \| Beta 2 microglobulin \| Beta 2 microglobulin precursor \| Beta chain of mhc class 1 proteins \| Beta chain of MHC class I molecules \| Beta-2-microglobulin form pI 5.3 \| CDABP0092 \| Hdcma22p \| IMD43
规格或纯度	ActiBioPure™, Bioactive, Animal Free, Carrier Free, Azide Free, ≥95%(SDS-PAGE)
产品介绍	Purity ≥95% SDS-PAGE. Endotoxin level <0.1 EU/µg Function Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
生物活性	Immobilized Recombinant Human B2M at 2 μg/mL (100 μL/well) can bind Recombinant Human CD8 alpha with a linear range of 30-350 ng/mL.
内毒素水平	<0.1 EU/μg
表达系统	HEK293
种属	人(Human)
氨基酸	21-119 aa
序列	IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDMHHHHHH
纯度	≥95% (SDS-PAGE)
蛋白标签	C-His
无载体	Yes
无动物源	Yes
Accession #	P61769
来源	重组表达
预测分子量	12 kDa
SDS-PAGE	13 kDa under reducing conditions.

储存与运输

物理形态	冻干(Lyophilized)
储存缓冲液	Lyophilized from 10mM PBS, 5% trehalose, 5% mannitol, pH7.4.
复溶	Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml.
储存温度	-20°C储存,避免反复冻融
运输条件	超低温冰袋运输
稳定性与储存	Store at -20~-80℃ for more than 1 year. Upon delivery aliquot. Avoid freeze/thaw cycle.
分子类型	蛋白质

图片

Protein Bioactivity: Recombinant Human beta 2 Microglobulin Protein (H293861)

Recombinant Human beta 2 Microglobulin Protein (H293861) - Protein Bioactivity
Immobilized Recombinant Human beta 2 Microglobulin Protein (H293861) at 2.0 μg/mL can bind Recombinant Human CD8 alpha with a linear range of 30 - 350 ng/mL. The ED₅₀ for this effect is 212.5 ng/mL.

SDS-PAGE: Recombinant Human beta 2 Microglobulin Protein (H293861)

Recombinant Human beta 2 Microglobulin Protein (H293861) - SDS-PAGE
3 μg/lane of Recombinant Human beta 2 Microglobulin Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.5 kDa.

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找到4个结果

批号(Lot Number)	证书类型	货号
ZJ24F0404713	分析证书	H293861
ZJ24F0404714	分析证书	H293861
ZJ24F0404881	分析证书	H293861
ZJ24F0404715	分析证书	H293861

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引用文献

1. Gerhard DS, Wagner L, Feingold EA, Shenmen CM, Grouse LH, Schuler G, Klein SL, Old S, Rasooly R, Good P et al.. (2004) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).. Genome Res, 14 (10B): (2121-7). [PMID:15489334]
2. Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K et al.. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs.. Nat Genet, 36 (1): (40-5). [PMID:14702039]
3. Thomas R Burkard,Melanie Planyavsky,Ines Kaupe,Florian P Breitwieser,Tilmann Bürckstümmer,Keiryn L Bennett,Giulio Superti-Furga,Jacques Colinge. (2011-01-29) Initial characterization of the human central proteome.. BMC systems biology, 5 (17-17). [PMID:21269460]
4. Argilés, A A, Derancourt, J J, Jauregui-Adell, J J, Mion, C C and Demaille, J G JG.. (1992) Biochemical characterization of serum and urinary beta 2 microglobulin in end-stage renal disease patients.. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, [PMID:1336137]
5. Okon, M M, Bray, P P and Vucelić, D D.. (1992) 1H NMR assignments and secondary structure of human beta 2-microglobulin in solution.. Biochemistry, (22): [PMID:1390678]
6. Saper, M A MA, Bjorkman, P J PJ and Wiley, D C DC.. (1991) Refined structure of the human histocompatibility antigen HLA-A2 at 2.6 A resolution.. Journal of molecular biology, (20): [PMID:2038058]
7. Bjorkman, P J PJ and 5 more authors.. (1987) Structure of the human class I histocompatibility antigen, HLA-A2.. Nature, [PMID:3309677]
8. Güssow, D D and 6 more authors.. (1987) The human beta 2-microglobulin gene. Primary structure and definition of the transcriptional unit.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:3312414]
9. Gorevic, P D PD and 8 more authors.. (1986) Polymerization of intact beta 2-microglobulin in tissue causes amyloidosis in patients on chronic hemodialysis.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:3532124]
10. Cunningham, B A BA, Wang, J L JL, Berggård, I I and Peterson, P A PA.. (1973) The complete amino acid sequence of beta 2-microglobulin.. Biochemistry, (20): [PMID:4586824]
11. Suggs, S V SV, Wallace, R B RB, Hirose, T T, Kawashima, E H EH and Itakura, K K.. (1981) Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.. Proceedings of the National Academy of Sciences of the United States of America, [PMID:6171820]
12. Momoi, T T and 5 more authors.. (1995) Amino acid sequence of a modified beta 2-microglobulin in renal failure patient urine and long-term dialysis patient blood.. Clinica chimica acta; international journal of clinical chemistry, (15): [PMID:7554280]
13. Collins, E J EJ, Garboczi, D N DN, Karpusas, M N MN and Wiley, D C DC.. (1995) The three-dimensional structure of a class I major histocompatibility complex molecule missing the alpha 3 domain of the heavy chain.. Proceedings of the National Academy of Sciences of the United States of America, (14): [PMID:7862664]
14. Miyata, T T and 7 more authors.. (1994) Glycation of human beta 2-microglobulin in patients with hemodialysis-associated amyloidosis: identification of the glycated sites.. Biochemistry, (11): [PMID:7918443]
15. Haag-Weber, M M, Mai, B B and Hörl, W H WH.. (1994) Isolation of a granulocyte inhibitory protein from uraemic patients with homology of beta 2-microglobulin.. Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, [PMID:8084451]
16. Smith, K J KJ and 6 more authors.. (1996) Bound water structure and polymorphic amino acids act together to allow the binding of different peptides to MHC class I HLA-B53.. Immunity, [PMID:8624812]
17. Braud, V M VM, Allan, D S DS, Wilson, D D and McMichael, A J AJ.. (1998) TAP- and tapasin-dependent HLA-E surface expression correlates with the binding of an MHC class I leader peptide.. Current biology : CB, (1): [PMID:9427624]
18. Wainwright, S D SD, Biro, P A PA and Holmes, C H CH.. (2000) HLA-F is a predominantly empty, intracellular, TAP-associated MHC class Ib protein with a restricted expression pattern.. Journal of immunology (Baltimore, Md. : 1950), (1): [PMID:10605026]
19. Rosa, F F and 5 more authors.. (1983) The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.. The EMBO journal, [PMID:11894933]
20. Trinh, Chi H CH, Smith, David P DP, Kalverda, Arnout P AP, Phillips, Simon E V SE and Radford, Sheena E SE.. (2002) Crystal structure of monomeric human beta-2-microglobulin reveals clues to its amyloidogenic properties.. Proceedings of the National Academy of Sciences of the United States of America, (23): [PMID:12119416]
21. Stewart-Jones, Guillaume B E GB, McMichael, Andrew J AJ, Bell, John I JI, Stuart, David I DI and Jones, E Yvonne EY.. (2003) A structural basis for immunodominant human T cell receptor recognition.. Nature immunology, [PMID:12796775]
22. He, Xian-Hui XH, Xu, Li-Hui LH, Liu, Yi Y and Zeng, Yao-Ying YY.. (2004) [Cloning of human beta-microglobulin gene and its high expression in Escherichia coli].. Sheng wu gong cheng xue bao = Chinese journal of biotechnology, [PMID:16108498]
23. Eakin, Catherine M CM, Berman, Andrea J AJ and Miranker, Andrew D AD.. (2006) A native to amyloidogenic transition regulated by a backbone trigger.. Nature structural & molecular biology, [PMID:16491088]
24. Deng, Nan-Jie NJ, Yan, Lisa L, Singh, Deepak D and Cieplak, Piotr P.. (2006) Molecular basis for the Cu2+ binding-induced destabilization of beta2-microglobulin revealed by molecular dynamics simulation.. Biophysical journal, (1): [PMID:16513784]
25. Wani, Manzoor A MA and 8 more authors.. (2006) Familial hypercatabolic hypoproteinemia caused by deficiency of the neonatal Fc receptor, FcRn, due to a mutant beta2-microglobulin gene.. Proceedings of the National Academy of Sciences of the United States of America, (28): [PMID:16549777]
26. Kihara, Miho M and 7 more authors.. (2006) Conformation of amyloid fibrils of beta2-microglobulin probed by tryptophan mutagenesis.. The Journal of biological chemistry, (13): [PMID:16901902]
27. Shiroishi, Mitsunori M and 8 more authors.. (2006) Structural basis for recognition of the nonclassical MHC molecule HLA-G by the leukocyte Ig-like receptor B2 (LILRB2/LIR2/ILT4/CD85d).. Proceedings of the National Academy of Sciences of the United States of America, (31): [PMID:17056715]
28. Iwata, Kentaro K, Matsuura, Takanori T, Sakurai, Kazumasa K, Nakagawa, Atsushi A and Goto, Yuji Y.. (2007) High-resolution crystal structure of beta2-microglobulin formed at pH 7.0.. Journal of biochemistry, [PMID:17646174]
29. Esposito, Gennaro G and 16 more authors.. (2008) The controlling roles of Trp60 and Trp95 in beta2-microglobulin function, folding and amyloid aggregation properties.. Journal of molecular biology, (9): [PMID:18395224]
30. Ricagno, Stefano S and 7 more authors.. (2008) DE loop mutations affect beta2-microglobulin stability and amyloid aggregation.. Biochemical and biophysical research communications, (5): [PMID:18835253]
31. Ricagno, Stefano S, Raimondi, Sara S, Giorgetti, Sofia S, Bellotti, Vittorio V and Bolognesi, Martino M.. (2009) Human beta-2 microglobulin W60V mutant structure: Implications for stability and amyloid aggregation.. Biochemical and biophysical research communications, (13): [PMID:19284997]
32. Giorgetti, Sofia S and 17 more authors.. (2011) Effect of tetracyclines on the dynamics of formation and destructuration of beta2-microglobulin amyloid fibrils.. The Journal of biological chemistry, (21): [PMID:21068391]
33. Valleix, Sophie S and 25 more authors.. (2012) Hereditary systemic amyloidosis due to Asp76Asn variant β2-microglobulin.. The New England journal of medicine, (14): [PMID:22693999]
34. Bian, Yangyang Y and 9 more authors.. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.. Journal of proteomics, (16): [PMID:24275569]
35. Sreejit, Gopalkrishna G and 6 more authors.. (2014) The ESAT-6 protein of Mycobacterium tuberculosis interacts with beta-2-microglobulin (β2M) affecting antigen presentation function of macrophage.. PLoS pathogens, [PMID:25356553]

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体积（添加到小瓶中）

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所需的复溶浓度

Recombinant Human beta 2 Microglobulin Protein

库存信息

库存信息

库存信息

库存信息

基本描述

储存与运输

图片

质检证书（CoA,COO,BSE/TSE 和分析图谱)

可替换产品

引用文献

溶液计算器

摩尔浓度计算器

稀释计算器

复溶计算器