货号 (SKU)

包装规格

是否现货

价格

数量

D751553-1ml

1ml

现货

D751553-5ml

5ml

现货

D751553-60ml

60ml

期货

D751553-450ml

450ml

期货

基本描述

别名

NGS片段筛选磁珠

英文别名

DNA Fragselect XP Magnetic Beads

稳定性与储存

Store at 2-8℃ long term (12 months). Do not freeze.

英文名称

UltraBio™ DNA Size Selection Magnetic Beads

储存温度

2-8°C储存,禁止冷冻

运输条件

冰袋运输

产品介绍

UltraBio™ DNA片段分选磁珠是基于固相载体可逆化固定(Solid Phase Reverse Immobilization, SPRI)技术，使用新型核酸纯化介质包被的磁珠，配合优化的缓冲体系，在特定比例的磁珠悬液中分离纯化出特定长度范围核酸片段的产品。适用于二代测序建库过程中DNA片段的分选与纯化，可根据不同的加样条件，得到不同大小的DNA文库。

使用说明：

一、自备试剂和器材

1、80%乙醇

2、洗脱液：10mM Tris-HCl（pH8.0）或无菌水

3、设备：移液器、涡旋振荡器、磁力架、离心管

二、DNA片段分选操作步骤

1、一次结合：将样本加入1.5mL离心管中，参照下表1加入特定体积的磁珠悬液，涡旋混匀后室温静置10min。将离心管置于磁力架上至溶液完全澄清，将上清转移到一新的1.5mL离心管中，弃去磁珠。

注：磁珠悬液加入量=样本体积*第一次结合比率。

2、二次结合：参照下表1，在上述新的离心管中加入特定比率的磁珠悬液，涡旋混匀后室温静置 10min。将离心管置于磁力架上至溶液完全澄清，弃去上清。

注：磁珠悬液加入量=样本体积*第二次结合比率。

3、清洗1：保持离心管固定于磁力架上，加入200μL 80%乙醇，静置1min，无需重悬磁珠，弃去上清（保持离心管固定于磁力架上）。

4、清洗2：重复步骤3一次。

5、除醇：保持离心管置于磁力架上，静置通风2-5min除醇。

注：除醇过程中请勿过分干燥磁珠，否则会降低纯化效率。

6、洗脱：将离心管从磁力架上取下，加入30μL洗脱液洗脱，涡旋混匀后室温静置10min。

7、转移核酸：将离心管置于磁力架上，静置2-5min，待磁珠完全吸附后将上清转移至新样品管中备用。

分选片段大小	200-300bp	300-400bp	400-500bp	500-600bp	600-800bp
第一次结合比率	0.80×	0.70×	0.60×	0.55×	0.50×
第二次结合比率	0.20×	0.20×	0.20×	0.15×	0.15×

表1 分选DNA片段大小与磁珠悬液加入量比率

三、PCR产物纯化操作步骤

1、结合：向待纯化的PCR产物样本中加入同等样本体积的磁珠悬液，涡旋混匀后，室温静置5min。将离心管置于磁力架上至溶液完全澄清，弃去上清。

2、清洗1：保持离心管固定于磁力架上，加入200μL 80%乙醇，静置1min，无需重悬磁珠，弃去上清（保持离心管固定于磁力架上）。

3、清洗2：重复步骤2一次。

4、除醇：保持离心管置于磁力架上，静置通风2-5min除醇。

注：除醇过程中请勿过分干燥磁珠，否则会降低纯化效率。

5、洗脱：将离心管从磁力架上取下，加入20-100μL的洗脱液，涡旋混匀后，室温静置5min。

6、转移核酸：将离心管置于磁力架上，静置2-5min，待磁珠完全吸附后将上清转移至新的离心管中备用。

注意事项：

1、本试剂为磁珠悬液，严禁冷冻、离心，第一次使用前需充分涡旋混匀；

2、建议样本体积至少为100μL，从而降低加样误差；若样本体积不足100μL可用水补足；

3、磁珠使用前须提前半小时取出，平衡至室温；

4、磁珠请勿过分干燥，否则会降低洗脱效率。

UltraBio™ DNA Size Selection Magnetic Beads utilize Solid Phase Reverse Immobilization (SPRI) technology. These beads are coated with a novel nucleic acid purification medium and combined with an optimized buffer system. At specific bead-to-sample ratios, they enable the isolation and purification of nucleic acid fragments within defined size ranges. This product is designed for DNA fragment size selection and purification during next-generation sequencing (NGS) library preparation, allowing users to obtain DNA libraries of varying sizes by adjusting the sample loading conditions.

Instructions for Use:

I. User-Supplied Reagents and Equipment

1.80% Ethanol

2.Elution Buffer: 10 mM Tris-HCl (pH 8.0) or Nuclease-Free Water

3.Equipment: Pipettes, Vortex mixer, Magnetic rack, Microcentrifuge tubes (1.5 mL)

II. DNA Fragment Size Selection Procedure

1.First Binding:

Transfer the sample to a 1.5 mL microcentrifuge tube.

Add the specified volume of Bead Suspension according to Table 1 (Bead Suspension Volume = Sample Volume × First Binding Ratio).

Vortex-mix thoroughly. Incubate at room temperature for 10 minutes.

Place the tube on the magnetic rack until the solution clears completely.

Transfer the supernatant to a new 1.5 mL microcentrifuge tube. Discard the beads.

2.Second Binding:

Add the specified volume of Bead Suspension to the supernatant from Step 1 according to Table 1 (Bead Suspension Volume = Original Sample Volume × Second Binding Ratio).

Vortex-mix thoroughly. Incubate at room temperature for 10 minutes.

Place the tube on the magnetic rack until the solution clears completely. Discard the supernatant.

3.Wash 1:

With the tube on the magnetic rack, add 200 μL of 80% Ethanol. Do NOT resuspend the beads.

Incubate for 1 minute.

Carefully aspirate and discard the supernatant while keeping the tube on the magnetic rack.

4.Wash 2:

Repeat Step 3 once.

5.Ethanol Removal:

Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.

Note: Avoid over-drying the beads, as this reduces purification efficiency.

6.Elution:

Remove the tube from the magnetic rack.

Add 30 μL Elution Buffer. Vortex-mix thoroughly to resuspend the beads.

Incubate at room temperature for 10 minutes.

7.Nucleic Acid Recovery:

Place the tube back on the magnetic rack for 2-5 minutes until the solution clears completely.

Carefully transfer the supernatant (containing the purified DNA) to a new tube.

Table 1: DNA Size Selection Ranges and Bead Suspension Addition Ratios

Size Selection Range	200-300bp	300-400bp	400-500bp	500-600bp	600-800bp
First Binding Ratio	0.80×	0.70×	0.60×	0.55×	0.50×
Second Binding Ratio	0.20×	0.20×	0.20×	0.15×	0.15×

III. PCR Product Purification Procedure

1.Binding:

Add an equal volume of Bead Suspension to the PCR product sample.

Vortex-mix thoroughly. Incubate at room temperature for 5 minutes.

Place the tube on the magnetic rack until the solution clears completely. Discard the supernatant.

2.Wash 1:

With the tube on the magnetic rack, add 200 μL of 80% Ethanol. Do NOT resuspend the beads.

Incubate for 1 minute.

Carefully aspirate and discard the supernatant while keeping the tube on the magnetic rack.

3. Wash 2:

Repeat Step 2 once.

Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.

Note: Avoid over-drying the beads, as this reduces purification efficiency.

4.Ethanol Removal:

Keep the tube on the magnetic rack. Air-dry the beads at room temperature for 2-5 minutes.

Note: Avoid over-drying the beads, as this reduces purification efficiency.

5.Elution:

Remove the tube from the magnetic rack.

Add 20-100 μL Elution Buffer (user-defined volume). Vortex-mix thoroughly to resuspend the beads.

Incubate at room temperature for 5 minutes.

6.Nucleic Acid Recovery:

Place the tube back on the magnetic rack for 2-5 minutes until the solution clears completely.

Carefully transfer the supernatant (containing the purified PCR product) to a new tube.

Precautions:

1.Storage & Handling: This reagent is supplied as a Bead Suspension. DO NOT freeze or centrifuge the suspension. Vortex-mix thoroughly before initial use.

2.Sample Volume: Minimum recommended sample volume: ≥100 μL (to minimize pipetting error). For samples <100 μL, adjust to 100 μL using Nuclease-Free Water.

3.Pre-Use Equilibration: Equilibrate the Bead Suspension to room temperature (RT) for ≥30 minutes before use.

4.Bead Drying: Avoid over-drying the beads during the drying step, as this will significantly reduce elution efficiency.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ25F0926444	分析证书	D751553
ZJ25F0926443	分析证书	D751553

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基本描述

质检证书（CoA,COO,BSE/TSE 和分析图谱)

可替换产品

溶液计算器

摩尔浓度计算器

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