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基本描述
| 产品名称 | 荧光素酶 来源于大肠杆菌 |
|---|---|
| 英文别名 | Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) | firefly luciferase | luciferase (firefly luciferin) | Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing) | firefly luciferin luciferase | Photinus pyralis luciferase |
| 规格或纯度 | EnzymoPure™, 重组, 1 mg/ml，＞ 1.0 x 10^10 relative light units (RLU)/ml Specific activity: 1.9 x 10^11 RLU/mg purified protein |
| 产品介绍 |
分子量：ca. 70 kDa Product introduction Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging. Application The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase. |
储存与运输
| 浓度 | 1 mg/ml，＞ 1.0 x 10^10 relative light units (RLU)/ml Specific activity: 1.9 x 10^11 RLU/mg purified protein |
|---|---|
| 储存温度 | -20°C储存 |
| 运输条件 | 超低温冰袋运输 |
| CAS编号和信息 | 61970-00-1 |
| 酶学委员会编号 | 1.13.12.7 |
| 单位定义 | One relative light unit (RLU) is defined as the amount of enzyme which emits 1 count of light for 20 s at 30°C and pH 7.8. |
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