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Recombinant CD15/SSEA-1 Antibody (AF555)

    应用:
  • Flow Cytometry
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: P52C45
  • 偶联: AF555
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab223938-10T
10T 现货 Stock Image
Ab223938-25T
25T 现货 Stock Image
Ab223938-100T
100T 现货 Stock Image

基本信息

产品名称 Recombinant CD15/SSEA-1 Antibody (AF555)
别名 阶段特异性胚胎抗原-1 | CD15抗体 | FUT4抗体 | Anti-CD15/SSEA-1抗体 | 小鼠抗人CD15抗体
英文别名 3)-fucosyltransferase antibody | Alpha-(1 antibody | Alpha-(1,3)-fucosyltransferase antibody | Alpha-3-fucosyltransferase antibody | ELAM-1 ligand fucosyltransferase antibody | ELFT antibody | FCT3A antibody | Fuc-TIV antibody | Fucosyltransferase 4 (Alph
规格或纯度 ExactAb™, 已验证, 重组, 5 μL/test
生化机理 Isoform Short: Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins. Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins. Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (By similarity). In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE. Isoform Long: Does not generate Lewis X antigens.
宿主种属 小鼠(Mouse)
特异性 Human CD15
种属反应性 人(Human)
免疫原 WERI-RB-1 retinoblastoma cell line
阳性对照 Flow Cytometry: Human peripheral blood granulocytes
偶联 AF555
Excitation(nm) Green Laser(532nm)
Emission(nm) 568nm
Ex/Em(nm) Ex:553nm;Em:568nm

产品属性

克隆类型 重组抗体
Format Whole IgG
轻链亚型 kappa
纯化方法 Protein A purified
储存缓冲液 Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 5 μL/test
储存温度 2-8°C储存,避光,禁止冷冻
运输条件 冰袋运输
稳定性与储存 The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型 抗体

关联靶点(人)

FUT4 Tbio α-(1,3)-岩藻糖基转移酶 4(Alpha-(1,3)-fucosyltransferase 4) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Recombinant CD15/SSEA-1 Antibody (AF555) (Ab223938) - Flow Cytometry
Flow cytometry analysis of CD15/SSEA-1 expression by human peripheral blood granulocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD15/SSEA-1 Antibody (AF555) (Ab223938) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF555) (Ab190246). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称 稀释比例
Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

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找到3个结果

批号(Lot Number) 证书类型 货号
ZJ25F0927749 分析证书 Ab223938
ZJ25F0927748 分析证书 Ab223938
ZJ25F0927747 分析证书 Ab223938

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