Recombinant CD4 Antibody (AF750)

COA

级别和纯度:

重组

已验证

ExactAb™

0.5mg/mL

应用:

Flow Cytometry

宿主种属: 大鼠(Rat)
种属反应性: 小鼠(Mouse)
亚型: Rat IgG2b
克隆号: P48A42
偶联: AF750

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货号 (SKU)	包装规格	是否现货
Ab223919-10μg	10μg	现货
Ab223919-25μg	25μg	现货
Ab223919-100μg	100μg	现货

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点击查看靶蛋白信息: CD4

Accession#:P06332 (20) AF750 (36) Cd4 (49) Gene ID:12504 (20)

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基本信息

产品名称	Recombinant CD4 Antibody (AF750)
规格或纯度	ExactAb™, 已验证, 重组, 0.5mg/mL
生化机理	Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. Post-translational modification Palmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. Phosphorylated by PKC; phosphorylation plays an important role for CD4 internalization.
宿主种属	大鼠(Rat)
特异性	Mouse CD4
种属反应性	小鼠(Mouse)
免疫原	Mouse CTL clone V4
阳性对照	Flow Cytometry: C57BL/6 mouse splenocytes
偶联	AF750
Excitation(nm)	Red Laser(633nm)
Emission(nm)	776nm
Ex/Em(nm)	Ex:752nm;Em:776nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	0.5mg/mL
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

CD4 Tclin T 细胞表面糖蛋白 CD4(T-cell surface glycoprotein CD4) (0 活性数据)

点击查看靶蛋白信息： CD4

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD4 Antibody (AF750) (Ab223919)

Recombinant CD4 Antibody (AF750) (Ab223919) - Flow Cytometry
Flow cytometry analysis of CD4 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD4 Antibody (AF750) (Ab223919) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF750) (Ab190243).

Flow Cytometry: Recombinant CD4 Antibody (AF750) (Ab223919)

Recombinant CD4 Antibody (AF750) (Ab223919) - Flow Cytometry
Flow cytometry analysis of CD4 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD4 Antibody (AF750) (Ab223919) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF750) (Ab190243). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	≤1.0μg/ test For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.

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批号(Lot Number)	证书类型	货号
ZJ25F0926986	分析证书	Ab223919
ZJ25F0926985	分析证书	Ab223919
ZJ25F0926987	分析证书	Ab223919

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