首页
抗体
一抗
偶联一抗
Recombinant HLA-DR Antibody (PE-Cy5.5)

Recombinant HLA-DR Antibody (PE-Cy5.5)

级别和纯度:

5 μL/test

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2a
克隆号: P22B24
偶联: PE-Cy5.5

有货

库存信息

关闭

库存信息

关闭

库存信息

关闭

货号 (SKU)	包装规格	是否现货
Ab223650-10T	10T	现货
Ab223650-25T	25T	现货
Ab223650-100T	100T	现货

查看相关系列

点击查看靶蛋白信息: HLA-DRA

Accession#:P01903 (24) Gene ID:3122 (24) HLA-DR (20) HLA-DRA (24) PE-Cy5.5 (10) 串联染料 (162)

Skip to the end of the images gallery

Skip to the beginning of the images gallery

基本信息

产品名称	Recombinant HLA-DR Antibody (PE-Cy5.5)
别名	抗人类白细胞抗原-DR \| HLA-DR抗体 \| Anti-HLA-DR抗体 \| 小鼠抗人HLA_DR抗体
英文别名	FLJ51114 \| histocompatibility antigen HLA-DR alpha \| HLA class II histocompatibility antigen, DR alpha chain \| HLADR \| HLA-DR \| HLA-DRA \| HLA-DRA1 \| Mouse anti-human HLA-DR antibody \| HLA-DR Monoclonal Antibody \| Anti-HLA-DR \| Human HLA-DR antibody \| HLA-
规格或纯度	ExactAb™, 已验证, 重组, 5 μL/test
生化机理	An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.
宿主种属	小鼠(Mouse)
特异性	Human HLA-DR
种属反应性	人(Human)
免疫原	Human lymphoblastoid B-cell line RPMI 8866
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes; Raji cells
偶联	PE-Cy5.5
Excitation(nm)	Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)
Emission(nm)	696nm
Ex/Em(nm)	Ex:565nm;Em:696nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

HLA-DRA Tbio HLA II 类组织相容性抗原，DR α 链(HLA class II histocompatibility antigen, DR alpha chain) (0 活性数据)

点击查看靶蛋白信息： HLA-DRA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650)

Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (PE-Cy5.5) (Ab216765).

Flow Cytometry: Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650)

Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5.5) (Ab216765). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650)

Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (PE-Cy5.5) (Ab223650) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (PE-Cy5.5) (Ab216765). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0825119	分析证书	Ab223650
ZJ25F0825120	分析证书	Ab223650
ZJ25F0825118	分析证书	Ab223650

溶液计算器

摩尔浓度计算器

计算溶液所需的质量、体积或浓度。

质量

浓度

体积

分子量

g/mol

稀释计算器

计算配制储备溶液所需的稀释度。

浓度1（C1）

体积 1 (V1)

浓度 2 (C2)

体积 2 (V2)

复溶计算器

复溶计算器允许您快速计算试剂的体积，以复溶小瓶。只需输入试剂的质量和目标浓度，计算器将确定其余部分。

体积（添加到小瓶中）

质量（小瓶）

所需的复溶浓度