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Recombinant HLA-DR Antibody (AF660)

Recombinant HLA-DR Antibody (AF660)

级别和纯度:

5 μL/test

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG2a
克隆号: P22B24
偶联: AF660

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货号 (SKU)	包装规格	是否现货
Ab223636-10T	10T	现货
Ab223636-25T	25T	现货
Ab223636-100T	100T	现货

查看相关系列

点击查看靶蛋白信息: HLA-DRA

Accession#:P01903 (24) AF660 (26) Gene ID:3122 (24) HLA-DR (20) HLA-DRA (24)

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基本信息

产品名称	Recombinant HLA-DR Antibody (AF660)
别名	抗人类白细胞抗原-DR \| HLA-DR抗体 \| Anti-HLA-DR抗体 \| 小鼠抗人HLA_DR抗体
英文别名	FLJ51114 \| histocompatibility antigen HLA-DR alpha \| HLA class II histocompatibility antigen \| DR alpha chain \| HLADR \| HLA-DR \| HLA-DRA \| HLA-DRA1 \| major histocompatibility complex \| class II \| DR alpha \| MHC cell surface glycoprotein \| MHC class II ant
规格或纯度	重组, ExactAb™, 已验证, 5 μL/test
生化机理	An alpha chain of antigen-presenting major histocompatibility complex class II (MHCII) molecule. In complex with the beta chain HLA-DRB, displays antigenic peptides on professional antigen presenting cells (APCs) for recognition by alpha-beta T cell receptor (TCR) on HLA-DR-restricted CD4-positive T cells. This guides antigen-specific T-helper effector functions, both antibody-mediated immune response and macrophage activation, to ultimately eliminate the infectious agents and transformed cells. Typically presents extracellular peptide antigens of 10 to 30 amino acids that arise from proteolysis of endocytosed antigens in lysosomes. In the tumor microenvironment, presents antigenic peptides that are primarily generated in tumor-resident APCs likely via phagocytosis of apoptotic tumor cells or macropinocytosis of secreted tumor proteins. Presents peptides derived from intracellular proteins that are trapped in autolysosomes after macroautophagy, a mechanism especially relevant for T cell selection in the thymus and central immune tolerance. The selection of the immunodominant epitopes follows two processing modes: 'bind first, cut/trim later' for pathogen-derived antigenic peptides and 'cut first, bind later' for autoantigens/self-peptides. The anchor residue at position 1 of the peptide N-terminus, usually a large hydrophobic residue, is essential for high affinity interaction with MHCII molecules.
宿主种属	小鼠(Mouse)
特异性	Human HLA-DR
种属反应性	人(Human)
免疫原	Human lymphoblastoid B-cell line RPMI 8866
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes; Raji cells
偶联	AF660
Excitation(nm)	Red Laser(633nm)
Emission(nm)	691nm
Ex/Em(nm)	Ex:663nm;Em:691nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冷冻
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

HLA-DRA Tbio HLA II 类组织相容性抗原，DR α 链(HLA class II histocompatibility antigen, DR alpha chain) (0 活性数据)

点击查看靶蛋白信息： HLA-DRA

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant HLA-DR Antibody (AF660) (Ab223636)

Recombinant HLA-DR Antibody (AF660) (Ab223636) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant HLA-DR Antibody (AF660) (Ab223636) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF660) (Ab225744).

Flow Cytometry: Recombinant HLA-DR Antibody (AF660) (Ab223636)

Recombinant HLA-DR Antibody (AF660) (Ab223636) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (AF660) (Ab223636) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF660) (Ab225744). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Recombinant HLA-DR Antibody (AF660) (Ab223636)

Recombinant HLA-DR Antibody (AF660) (Ab223636) - Flow Cytometry
Flow cytometry analysis of HLA-DR expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant HLA-DR Antibody (AF660) (Ab223636) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF660) (Ab225744). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0825867	分析证书	Ab223636
ZJ25F0825868	分析证书	Ab223636
ZJ25F0825869	分析证书	Ab223636

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