Recombinant CD3 Antibody (AF488)

COA

级别和纯度:

重组

已验证

ExactAb™

5 μL/test

应用:

Flow Cytometry

宿主种属: 小鼠(Mouse)
种属反应性: 人(Human)
亚型: Mouse IgG1
克隆号: P93B35
偶联: AF488

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货号 (SKU)	包装规格	是否现货
Ab223447-10T	10T	现货
Ab223447-25T	25T	现货
Ab223447-100T	100T	现货

查看相关系列

点击查看靶蛋白信息: CD3E

Accession#:P07766 (43) AF488 (107) CD3E (62) Gene ID:916 (41) T3E (33)

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基本信息

产品名称	Recombinant CD3 Antibody (AF488)
别名	白细胞分化抗原3 \| 分化簇3 \| T3复合物 \| T 细胞受体复合物的 CD3 epsilon 亚基 \| CD3e \| CD3 ε 抗体 \| Anti-CD3e抗体 \| 小鼠抗人CD3 epsilon抗体
英文别名	CD3 epsilon antibody \| CD3e antibody \| CD3e antigen antibody \| CD3e antigen epsilon polypeptide (TiT3 complex) antibody \| CD3E antigen epsilon polypeptide antibody \| CD3E antigen, epsilon subunit antibody \| CD3e molecule epsilon antibody \| CD3e molecule,
规格或纯度	ExactAb™, 已验证, 重组, 5 μL/test
生化机理	Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx.
宿主种属	小鼠(Mouse)
特异性	Human CD3ε
种属反应性	人(Human)
免疫原	Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
阳性对照	Flow Cytometry: Human peripheral blood lymphocytes
偶联	AF488
Excitation(nm)	Blue Laser(488nm)
Emission(nm)	525nm
Ex/Em(nm)	Ex:490nm;Em:525nm

产品属性

克隆类型	重组抗体
Format	Whole IgG
轻链亚型	kappa
纯化方法	Protein A purified
储存缓冲液	Liquid in PBS pH 7.4, containing 1% BSA and 0.05% Proclin300.
防腐剂	0.05% Proclin300
浓度	5 μL/test
储存温度	2-8°C储存,避光,禁止冷冻
运输条件	冰袋运输
稳定性与储存	The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
分子类型	抗体

关联靶点（人）

CD3E Tclin 细胞表面糖蛋白 CD3 ε 链(T-cell surface glycoprotein CD3 epsilon chain) (0 活性数据)

点击查看靶蛋白信息： CD3E

活性类型	活性值-log(M)	作用机制	期刊	参考文献(PubMed IDs)

图片

Flow Cytometry: Recombinant CD3 Antibody (AF488) (Ab223447)

Recombinant CD3 Antibody (AF488) (Ab223447) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (AF488) (Ab223447) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG (AF488) (Ab190245).

Flow Cytometry: Recombinant CD3 Antibody (AF488) (Ab223447)

Recombinant CD3 Antibody (AF488) (Ab223447) - Flow Cytometry
Flow cytometry analysis of CD3 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD3 Antibody (AF488) (Ab223447) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG (AF488) (Ab190245). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称	稀释比例
Flow Cytometry	5μl/test For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

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找到3个结果

批号(Lot Number)	证书类型	货号
ZJ25F0927772	分析证书	Ab223447
ZJ25F0927771	分析证书	Ab223447
ZJ25F0927773	分析证书	Ab223447

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