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| 货号 (SKU) | 包装规格 | 是否现货 | 价格 | 数量 |
|---|---|---|---|---|
| Ab219268-10μl |
10μl |
现货 ![]() |
| |
| Ab219268-50μl |
50μL |
现货 ![]() |
| |
| Ab219268-100μl |
100μL |
现货 ![]() |
| |
| Ab219268-1ml |
1ml |
期货 ![]() |
|
| 产品名称 | Hsp60 Mouse mAb (AF405) |
|---|---|
| 别名 | 60 kD热休克蛋白 | 热休克蛋白60抗体 | 热激蛋白60 | Anti-Hsp60抗体 | Hsp60小鼠单克隆抗体 | Hsp60抗体 |
| 英文别名 | 60 kDa chaperonin antibody | 60 kDa heat shock protein, mitochondrial antibody | CH60_HUMAN antibody | Chaperonin 60 antibody | Chaperonin, 60-KD antibody | CPN60 antibody | fa04a05 antibody | GROEL antibody | heat shock 60kDa protein 1 (chaperonin) antib |
| 规格或纯度 | ExactAb™, 已验证, 0.5 mg/mL |
| 宿主种属 | 小鼠(Mouse) |
| 特异性 | Hsp60 |
| 种属反应性 | 人(Human) |
| 免疫原 | Recombinant full length protein corresponding to Human Hsp60 |
| 阳性对照 | IF/ICC: Hela cells Flow Cytometry: HEK293 cells |
| 偶联 | AF405 |
| Excitation(nm) | Violet Laser(405nm) |
| Emission(nm) | 422nm |
| Ex/Em(nm) | Ex:401nm;Em:422nm |
| 克隆类型 | 单克隆抗体 |
|---|---|
| Format | Whole IgG |
| 轻链亚型 | kappa |
| 纯化方法 | Protein A purified |
| 储存缓冲液 | Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300. |
| 防腐剂 | 0.05% Proclin300 |
| 浓度 | 0.5 mg/mL |
| 储存温度 | 避光,-20°C储存,避免反复冻融 |
| 运输条件 | 超低温冰袋运输 |
| 稳定性与储存 | Store at -20°C. Protect from light. Avoid freeze/thaw cycles. |
| 分子类型 | 抗体 |
Hsp60 Mouse mAb (AF405) (Ab219268) - IF/ICC
IF analysis of Hsp60 (blue) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (AF405) (Ab219268) at a dilution of 1/1000 in blocking buffer overnight at 4°C in the dark. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.
Hsp60 Mouse mAb (AF405) (Ab219268) - Flow Cytometry
Flow cytometry analysis of Hsp60 expression by HEK293 cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Hsp60 Mouse mAb (AF405) (Ab219268) (red) at 1/500 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.
| 应用名称 | 稀释比例 |
|---|---|
| IF/ICC |
1/100-1/1000
|
| Flow Cytometry | 1/50 - 1/1000 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | Ab219268 | |
| 分析证书 | Ab219268 | |
| 分析证书 | Ab219268 |