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Hsp60 Mouse mAb (AF405)

    应用:
  • Flow Cytometry
  • IF/ICC
  • 宿主种属: 小鼠(Mouse)
  • 种属反应性: 人(Human)
  • 亚型: Mouse IgG1
  • 克隆号: EF02/2E10
  • 偶联: AF405
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货号 (SKU) 包装规格 是否现货 价格 数量
Ab219268-10μl
10μl 现货 Stock Image
Ab219268-50μl
50μL 现货 Stock Image
Ab219268-100μl
100μL 现货 Stock Image
Ab219268-1ml
1ml 期货 Stock Image

基本信息

产品名称 Hsp60 Mouse mAb (AF405)
别名 60 kD热休克蛋白 | 热休克蛋白60抗体 | 热激蛋白60 | Anti-Hsp60抗体 | Hsp60小鼠单克隆抗体 | Hsp60抗体
英文别名 60 kDa chaperonin antibody | 60 kDa heat shock protein, mitochondrial antibody | CH60_HUMAN antibody | Chaperonin 60 antibody | Chaperonin, 60-KD antibody | CPN60 antibody | fa04a05 antibody | GROEL antibody | heat shock 60kDa protein 1 (chaperonin) antib
规格或纯度 ExactAb™, 已验证, 0.5 mg/mL
宿主种属 小鼠(Mouse)
特异性 Hsp60
种属反应性 人(Human)
免疫原 Recombinant full length protein corresponding to Human Hsp60
阳性对照 IF/ICC: Hela cells Flow Cytometry: HEK293 cells
偶联 AF405
Excitation(nm) Violet Laser(405nm)
Emission(nm) 422nm
Ex/Em(nm) Ex:401nm;Em:422nm

产品属性

克隆类型 单克隆抗体
Format Whole IgG
轻链亚型 kappa
纯化方法 Protein A purified
储存缓冲液 Liquid in PBS pH 7.4, containing 50% glycerol, 1% BSA and 0.05% Proclin300.
防腐剂 0.05% Proclin300
浓度 0.5 mg/mL
储存温度 避光,-20°C储存,避免反复冻融
运输条件 超低温冰袋运输
稳定性与储存 Store at -20°C. Protect from light. Avoid freeze/thaw cycles.
分子类型 抗体

关联靶点(人)

HSPD1 Tbio 60 kDa 热休克蛋白,线粒体(60 kDa heat shock protein, mitochondrial) (0 活性数据)
活性类型 活性值-log(M) 作用机制 期刊 参考文献(PubMed IDs)

图片

Hsp60 Mouse mAb (AF405) (Ab219268) - IF/ICC
IF analysis of Hsp60 (blue) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp60 Mouse mAb (AF405) (Ab219268) at a dilution of 1/1000 in blocking buffer overnight at 4°C in the dark. Cells were counterstained with PI (red). Images were taken on the confocal laser scanning microscope.

Hsp60 Mouse mAb (AF405) (Ab219268) - Flow Cytometry
Flow cytometry analysis of Hsp60 expression by HEK293 cells (red) compared to an isotype control (blue). Cells were fixed and permeabilized with 100% methanol and then stained with Hsp60 Mouse mAb (AF405) (Ab219268) (red) at 1/500 dilution for 30 minutes at 4°C in the dark. Blue - Mouse IgG (AF405) (Ab190076). Black - Unlabelled control, cells without incubation with primary antibody.

应用

应用名称 稀释比例
IF/ICC

1/100-1/1000

Flow Cytometry

1/50 - 1/1000

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批号(Lot Number) 证书类型 货号
ZJ25F0623309 分析证书 Ab219268
ZJ25F0623308 分析证书 Ab219268
ZJ25F0623307 分析证书 Ab219268

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